• The Plant Pathology Journal
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• 제24권3호
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• pp.269-278
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• 2008

Ripe rot was frequently observed on fruits, leaves and stems of grape growing in eight locations in Korea from 2004 to 2006. All 30 isolates of Colletotrichum sp. were obtained from lesions of the ripe rot on grape plants. Out of the isolates, 19 isolates were identified as Colletotrichum acutatum and the others as Colletotrichum gloeosporioides based on morphological and cultural characteristics. Inter and intra specific variations of the Colletotrichum spp. isolates were investigated using RAPD and sequences of rDNA ITS and $\beta$-tubulin-2. Isolates of C. acutatum and C. gloeosporioides were distinctly differentiated by molecular analyses. Phylogenetic trees of ITS and$\beta$-tubulin-2 showed that Korean isolates of C. acutatum were clustered into groups A2 and A3 among the eight global groups. A2 included non-chromogenic isolates and A3 chromogenic ones. Both C. acutatum and C. gloeosporioides isolates were tested for pathogenicity to grape leaves. All isolates tested induced lesions on the leaves of grape by artificial inoculation. There was no difference in pathogenicity between C. acutatum and C. gloeosporioides isolates. This is the first report that C. acutatum except C. gloeosporioides causes grape ripe rot in Korea.

• 한국균학회지
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• 제48권3호
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• pp.297-312
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• 2020

2014년부터 2016년까지 국내 콩 재배 포장에서 시들음 증상을 보이는 시료에서 진균을 분리한 결과, Fusarium spp., Colletotrichum spp., Rhizoctonia spp., Macrophomina sp., Phytophthora spp., Calonectria ilicicola가 분리되었고, Fusarium균이 79.1%로 가장 많이 분리되었다. 53개의 Fusarium균을 균학적 특성에 의해 동정한 결과, F. solani, F. oxysporum, F. graminearum, F. fujikuroi 등 4개의 종 복합체(species complex)로 동정되었다. 염기서열분석을 통한 종 동정을 위해 translation elongation factor 1 alpha (TEF) 유전자 부위를 계통분석한 결과, F. solani, F. oxysporum, F. commune, F. asiaticum, F. fujikuroi 등 5종으로 확인되었다. 44개 Fusarium균주의 병원성을 확인하기 위하여 콩 3개 품종을 대상으로 유묘 침지 접종한 결과, F. asiaticum은 병원성이 없거나 미미한 것으로 나타났다. 병원성이 있는 10개 균주를 선발하여 기주범위를 조사한 결과, F. solani, F. oxysporum, F. commune은 콩에만 병원성이 있었고, F. fujikuroi는 콩, 강낭콩, 황동부콩, 흑동부콩 및 팥에 병원성을 보였다. 또한 13개 콩 품종을 대상으로 저항성 검정을 수행한 결과, F. commune과 F. fujikuroi는 모든 품종에 병을 일으켰다. 장기콩, 풍산나물콩, 소청자콩은 Fusarium에 의한 시들음병에 비교적 저항성이 것으로 나타났고, 황금올콩, 참올콩은 감수성 품종으로 확인되었다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.37-37
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• 2014

Glomerella leaf spot pathogens can infect apple leaves, causing extensive necrosis and premature defoliation, as well as necrotic spots on fruit. In recent years, the disease has been reported with increasing frequency in China, and appears to be spreading rapidly in some apple-producing areas. In this study, fungal isolates from diseased apples leaves collected in Henan and Shaanxi provinces were analyzed based on morphology, cultural characters, pathogenicity and molecular phylogenetics. It was found that Glomerella leaf spot of apple was caused by two pathogens, Colletotrichum fructicola and C. aenigma. Pathogenicity tests showed that C. fructicola and C. aenigma could infect apple leaves of cultivar Golden Delicious, as well as Gala, Qinguan, Pink Lady, Pacific Rose, Golden Century and Honeycrisp, all of which include Golden Delicious in their parentage. In wound inoculation experiments, C. fructicola and C. aenigma were pathogenic to fruit of Gala, Qinguan, Golden Delicious, Pacific Rose, Starkrimson and Fuji. With non-wounded fruit, C. fructicola was pathogenic to Gala and Golden Delicious, and C. aenigma was pathogenic to Gala. It is concluded that the two pathogens could be differentiated according to pathogenicity to leaves and fruits of different apple cultivars.

• 한국식물병리학회지
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• 제3권2호
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• pp.85-92
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• 1987

본 실험은 전기영동법에 의하여 탄저병균의 종분류를 하였다. 고추 탄저병균인 Colletotrichium gloeosporioides, C. dematium과 사과 탄저병균 Gloeosporium fructigenum은 esterase, leucine aminopeptidase, acid phosphatase와 glutamic oxaloacetic transaminase 동위효소에 의해 구분되었다. 특히 C. gloesoporioides의 G와 R-strain이 효소 pattern에 의해 구분되었다. G-strain은 고추의 모든 열매(푸른고추, 붉은고추)를 침해하나, R-strain은 단지 붉은 고추를 침해하고 푸른고추는 침해하지 않는다

• The Plant Pathology Journal
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• 제23권3호
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• pp.187-192
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• 2007

The anthracnose disease on pepper fruits in Korea was caused by Colletotrichum acutatum as well as C. gloeosporioides. Since C. acutatum showed less sensitivity to benomyl, it was analyzed whether the less sensitivity was given by the same mechanism for the fungicide resistance of C. gloeosporioides. The isolates of C. acutatum were less sensitive to the three benzimidazole fungicides tested, benomyl, carbendazim, and thiophanate-methyl. However, the of C. acutatum isolates were different from the resistant isolates of C. gloeosporioides in their response to diethofencarb, one of N-phenyl-carbamates; the former was still less sensitive to diethofencarb than the latter. The differences in the resistance mechanisms in two species were conspicuous in sequence analysis of the tub2 genes. The genes from C. acutatum did not show any non-synonymous base substitutions at the regions known to be correlated with the benzimidazole-resistance. All of these data may indicate that the less sensitivity of C. acutatum to benomyl is based on different mechanism(s) from that of C. gloeosporioides.

• 식물병연구
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• 제27권2호
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• pp.70-75
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• 2021

The abnormal brown sunken lesions were observed on cv. RubyS apple fruits in an orchard located in Gunwi, Gyeongbuk province, Korea. The primary observed symptoms such as small round sunken lesions and small black dots on the symptomatic area were different from the reported apple diseases. The affected apple fruits were sampled and subjected to isolation of the causal agent. Cultural and morphological characteristics of isolated fungal strain, designated KNUF-20GWA4, were similar to that of Colletotrichum spp. Based on multilocus sequence analyses using internal transcribed spacer regions and partial sequences of β-tubulin, glyceraldehyde-3-phosphate dehydrogenase, chitin synthase, and actin genes, strain KNUF-20GWA4 showed 99.2-100% similarities with C. aenigma ICMP 18608 and the isolate clustered together with several other strains of this species in the phylogenetic tree. To our knowledge, this is the first report of bitter rot on apple fruits caused by C. aenigma.

• Mycobiology
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• 제29권3호
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• pp.142-144
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• 2001

The tetrahydropahnatine alkaloid was assayed against spore germination of some saprophytic and pathogenic fungi e.g., Alternaria solani, A. brassicicola, A. brassicae, A. alternata, Erysiphe pisi, Curvularia lunata, C. pallescens, C. maculans, Curvularia species, Colletotrichum species, C. musae, Helminthosporium echinoclova, H. pennisetti, H. spiciferum, and Heterosporium sp. It inhibite spore germination of all the fungi tested. Colletotrichum spp. Curvularia lunata, Helminthosporium spiciferum and Heterosporium sp. were most sensitive as complete inhibition of spore germination was observed at very low concentration(200 ppm).

• The Plant Pathology Journal
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• 제36권6호
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• pp.570-578
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• 2020

Rhizopus rot is a serious postharvest disease of various crops caused by Rhizopus spp. and controlled mainly by synthetic fungicides. We detected the antifungal activity of a culture extract of Setosphaeria rostrata F3736 against Rhizopus oryzae. The active ingredient was identified as moriniafungin, a known sordarin derivative, which showed minimum inhibitory concentrations of 1-8 ㎍/ml against Colletotrichum spp. and 0.03-0.13 ㎍/ml against Rhizopus spp. in vitro. Moriniafungin showed protective control efficacies against Rhizopus rot on apple and peach fruits. Treatment with 25 ㎍/ml moriniafungin delimited the lesion diameter significantly by 100% on R. oryzae-inoculated apple fruits compared with the non-treated control. Treatment with 0.04 ㎍/ml of moriniafungin reduced the lesion diameter significantly by 56.45%, and treatment with higher concentrations of 0.2-25 ㎍/ml reduced the lesion diameter by 70-90% on Rhizopus stolonifer var. stolonifer-inoculated peach fruit. These results suggest moriniafungin has potential as a control agent of postharvest diseases caused by Rhizopus spp.

• The Plant Pathology Journal
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• 제38권6호
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• pp.629-636
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• 2022

Colletotrichum species is known as the major causal pathogen of red pepper anthracnose in Korea and various groups of fungicides are registered for the management of the disease. However, the consistent use of fungicides has resulted in the development of resistance in many red pepper-growing areas of Korea. Effective management of the occurrence of fungicide resistance depends on constant monitoring and early detection. Thus, in this study, various methods such as agar dilution method (ADM), gene sequencing, allele-specific polymerase chain reaction (PCR), and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were applied for the detection of benzimidazole resistance among 24 isolates of Colletotrichum acutatum s. lat. and Colletotrichum gloeosporioides s. lat. The result of the ADM showed that C. gloeosporioides s. lat. was classified into sensitive and resistant isolates to benomyl while C. acutatum s. lat. was insensitive at ≥1 ㎍/ml of benomyl. The sequence analysis of the β-tubulin gene showed the presence of a single nucleotide mutation at the 198th amino acid position of five isolates (16CACY14, 16CAYY19, 15HN5, 15KJ1, and 16CAYY7) of C. gloeosporioides s. lat. Allele-specific PCR and PCR-RFLP were used to detect point mutation at 198th amino acid position and this was done within a day unlike ADM which usually takes more than one week and thus saving time and resources that are essential in the fungicide resistance management in the field. Therefore, the molecular techniques established in this study can warrant early detection of benzimidazole fungicide resistance for the adoption of management strategies that can prevent yield losses among farmers.

• 농업과학연구
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• 제14권1호
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• pp.16-25
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• 1987

고추 종자에서 Colletotrichum acutatum, C. coccodes, C.dematium 및 C. gloeosporioides 등 4종의 탄저병균(炭疽病菌)이 검출(檢出)되었다. 그중 C.dematium과 C.gloeosporioides가 높은 비율(比率)로 검출(檢出)되었으며, 감염율(感染率)이 높은 종자시료(種子試料)는 각각(各各) 최고 84% 및 28%의 감염율(感染率)을 나타내었다. 반면에 C.acutatum과 C.coccodes는 각각 1개의 종자 시료(試料)에서 1% 및 2%의 낮은 비율(比率)로 검출(檢出)되었다. 표준습지법(標準濕紙法)이 냉동습지법(冷凍濕紙法)이나 한천배지배양법(培地培養法)에 비하여 종자상의 탄저병균(炭疽病菌) 검출(檢出)에 효과적(效果的)이었다. C.dematium과 C. gloeosporioides의 종자내 감염부위(感染部位)를 조사(調査)하였던 바, 종피(種皮)뿐 아니라 종자내 깊숙이 배유(胚乳)와 자엽(子葉)에 까지 감염(感染)되어 있음이 확인되었으나 종피(種皮) 감염율(感染率)이 높았다. C.dematium과 C.gloeosporioides 감염종자(感染種子)를 한천배지와 살균토양에 옮겨 발아율과 유묘생육상황(幼苗生育狀況)을 조사한 결과, 감염종자(感染種子)는 종자부패(種子腐敗), 모잘록, 유묘고사(幼苗枯死) 및 자엽(子葉)과 하배축(下胚軸)의 변색(變色)을 초래하였다. 접종실험(接種實驗) 결과 C.acutatum은 고추의 적과(赤果)에 병원성(病原性)이 있었고 C.coccodes는 고추 적과(赤果)에 강한 병원성(病原性)이, 고추 잎에 약한 병원성(病原性)이 있었다. C.dematium은 고추 잎과 청과(靑果)에 강한 병원성(病原性)이 있었고, C.gloeosporioides 는 고추 잎과 청과(靑果) 및 적과(赤果)에 병원성이 있었다. 이들 4종의 종자전염성(種子傳染性) 탄저병균(炭疽病菌)의 고추 이외의 다른 작물(作物)에 대한 병원성도 조사하였다.