Differentiation of Colletotrichum spp. Causing Anthracnose on Capsicum annum L. by Electrophoretic Method

전기영동법을 이용한 고추탄저병균의 분류

  • Park Won Mok (Department of Plant Protection, College of Agriculture, Korea University) ;
  • Park Sang Ho (Department of Plant Protection, College of Agriculture, Korea University) ;
  • Lee Yong Se (Department of Plant Protection, College of Agriculture, Korea University) ;
  • Ko Young Hee (Department of Plant Protection, College of Agriculture, Korea University) ;
  • Cho Eui Kyoo (Department of Plant Pathology, Institute of Agricultural Sciences, Rural Development Administration)
  • 박원목 (고려대학교 농과대학 식물보호학과) ;
  • 박상호 (고려대학교 농과대학 식물보호학과) ;
  • 이용세 (고려대학교 농과대학 식물보호학과) ;
  • 고영희 (고려대학교 농과대학 식물보호학과) ;
  • 조의규 (농촌진흥청 농업기술연구소 병리과)
  • Published : 1987.05.01

Abstract

The present researches were carried out to differentiate the species of Colletotrichum by elecrophoretic method. C. gloeosporioides, C. dematium and Gloeosporium fructigenum could be differentiated by the isozyme patterns of esterase, leucine aminopeptidase, acid phosphatase and glutamic oxaloacetic transaminase. Especially. G-strain and R-strain of C. gloeosporioides were differentiated by the enzyme patterns. The G­strain damaged all stage fruits (green and red fruits) of Capsicum annum. The R-strain could not infect the unripe (green) fruits, but it could damage only ripe (red) fruit of Capsicum annum.

본 실험은 전기영동법에 의하여 탄저병균의 종분류를 하였다. 고추 탄저병균인 Colletotrichium gloeosporioides, C. dematium과 사과 탄저병균 Gloeosporium fructigenum은 esterase, leucine aminopeptidase, acid phosphatase와 glutamic oxaloacetic transaminase 동위효소에 의해 구분되었다. 특히 C. gloesoporioides의 G와 R-strain이 효소 pattern에 의해 구분되었다. G-strain은 고추의 모든 열매(푸른고추, 붉은고추)를 침해하나, R-strain은 단지 붉은 고추를 침해하고 푸른고추는 침해하지 않는다

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