Browse > Article
http://dx.doi.org/10.5423/RPD.2021.27.2.70

Colletotrichum aenigma Associated with Apple Bitter Rot on Newly Bred cv. RubyS Apple  

Lee, Seung-Yeol (College of Agriculture and Life Sciences, Kyungpook National University)
Ten, Leonid N. (College of Agriculture and Life Sciences, Kyungpook National University)
Ryu, Jung-Joo (College of Agriculture and Life Sciences, Kyungpook National University)
Kang, In-Kyu (College of Agriculture and Life Sciences, Kyungpook National University)
Jung, Hee-Young (College of Agriculture and Life Sciences, Kyungpook National University)
Publication Information
Research in Plant Disease / v.27, no.2, 2021 , pp. 70-75 More about this Journal
Abstract
The abnormal brown sunken lesions were observed on cv. RubyS apple fruits in an orchard located in Gunwi, Gyeongbuk province, Korea. The primary observed symptoms such as small round sunken lesions and small black dots on the symptomatic area were different from the reported apple diseases. The affected apple fruits were sampled and subjected to isolation of the causal agent. Cultural and morphological characteristics of isolated fungal strain, designated KNUF-20GWA4, were similar to that of Colletotrichum spp. Based on multilocus sequence analyses using internal transcribed spacer regions and partial sequences of β-tubulin, glyceraldehyde-3-phosphate dehydrogenase, chitin synthase, and actin genes, strain KNUF-20GWA4 showed 99.2-100% similarities with C. aenigma ICMP 18608 and the isolate clustered together with several other strains of this species in the phylogenetic tree. To our knowledge, this is the first report of bitter rot on apple fruits caused by C. aenigma.
Keywords
Apple; Bitter rot; Colletotrichum aenigma; cv. RubyS;
Citations & Related Records
연도 인용수 순위
  • Reference
1 White, T. J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-322. Academic Press, San Diego, CA, USA.
2 Glass, N. L. and Donaldson, G. C. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl. Environ. Microbiol. 61: 1323-1330.   DOI
3 Guerber, J. C., Liu, B., Correll, J. C. and Johnston, P. R. 2003. Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility. Mycologia 95: 872-895.   DOI
4 Kumar, S., Stecher, G. and Tamura, K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33: 1870-1874.   DOI
5 Kwon, S.-I., Kim, J.-H., Kim, S.-A. and Kwon, Y.-S. 2019. 'RubyS', a small apple. HortScience 54: 2067-2069.   DOI
6 Oo, M. M. and Oh, S.-K. 2017. New record of anthracnose caused by Colletotrichum liriopes on broadleaf Liriope in Korea. Korean J. Mycol. 45: 68-73.   DOI
7 Sharma, G., Maymon, M. and Freeman, S. 2017. Epidemiology, pathology and identification of Colletotrichum including a novel species associated with avocado (Persea americana) anthracnose in Israel. Sci. Rep. 7: 15839.   DOI
8 Wang, Y.-C., Hao, X.-Y., Wang, L., Xiao, B., Wang, X.-C. and Yang, Y.- J. 2016. Diverse Colletotrichum species cause anthracnose of tea plants (Camellia sinensis (L.) O. Kuntze) in China. Sci. Rep. 6: 35287.   DOI
9 Choi, D.-W., Kim, D.-C. and Lim, C.-R. 2018. Analysis of factors influencing cultivation area of apple cultivars. J. Korean Soc. Rural Plan. 24: 25-31.   DOI
10 Wang, X., Liu, X., Wang, R., Fa, L., Chen, L., Xin, X. et al. 2021. First report of Colletotrichum aenigma causing walnut anthracnose in China. Plant Dis. 105: 225.
11 Choi, H.-W., Lee, Y. K. and Hong, S. K. 2017. First report of Colletotrichum aenigma causing anthracnose on Sedum kamtschaticum in Korea. Plant Dis. 101: 2150.   DOI
12 Meetum, P., Leksomboon, C. and Kanjanamaneesathian, M. 2015. First report of Colletotrichum aenigma and C. siamense, the causal agent of anthracnose disease of dragon fruit in Thailand. J. Plant Pathol. 97: 391-403.
13 FAOSTAT. 2019. Food and Agriculture Organization of the United Nations. URL http://www.fao.org/ [30 May 2021].
14 Fu, M., Crous, P. W., Bai, Q., Zhang, P. F., Xiang, J., Guo, Y. S. et al. 2019. Colletotrichum species associated with anthracnose of Pyrus spp. in China. Persoonia 42: 1-35.   DOI
15 Kim, J. S., Hassan, O. and Chang, T. 2021. First report of Colletotrichum aenigma causing anthracnose of grape in Korea. Plant. Dis. Advanced online publication. https://doi.org/10.1094/PDIS11-20-2458-PDN.
16 Oo, M. M., Yoon, H.-Y., Jang, H. A. and Oh, S.-K. 2018. Identification and characterization of Colletotrichum species associated with bitter rot disease of apple in South Korea. Plant Pathol. J. 34: 480-489.   DOI
17 Uhm, J. Y. 2010. Reduced Fungicide Spray Program for Major Apple Diseases Korea. Agriculture and Horticulture Press, Anyang, Korea. 251 pp.
18 Weir, B. S., Johnston, P. R. and Damm, U. 2012. The Colletotrichum gloeosporioides species complex. Stud. Mycol. 73: 115-180.   DOI
19 Carbone, I. and Kohn, L. M. 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91: 553-556.   DOI
20 Yan, J.-Y., Jayawardena, M. M. R. S., Goonasekara, I. D., Wang, Y., Zhang, W., Liu, M. et al. 2015. Diverse species of Colletotrichum associated with grapevine anthracnose in China. Fungal Divers. 71: 233-246.   DOI
21 Truong, H. H., Sato, T., Ishikawa, S., Minoshima, A., Nishimura, T. and Hirooka, Y. 2018. Three Colletotrichum species responsible for anthracnose on Synsepalum dulcificum (miracle fruit). Int. J. Phytopathol. 7: 89-101.   DOI