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http://dx.doi.org/10.4489/KJM.20200029

Diversity and Pathogenic Characteristics of Fusarium Species isolated from Wilted Soybeans in Korea  

Choi, Hyo-Won (Crop Protection Division, National Institute of Agricultural Sciences)
Kim, Seungnoh (Taean Agricultural Technology Center)
Hong, Sung Kee (Crop Protection Division, National Institute of Agricultural Sciences)
Publication Information
The Korean Journal of Mycology / v.48, no.3, 2020 , pp. 297-312 More about this Journal
Abstract
Wilted soybean plants were collected from soybeans cultivation fields in Korea from 2014 to 2016. Fusarium spp., Colletotrichum spp., Rhizoctonia spp., Macrophomina sp., Phytophthora spp., and Calonectria ilicicola were obtained from the infected samples. Out of these, Fusarium spp. were the dominant species (79.1%). In total, 53 isolates were identified as F. solani species complex, F. oxysporum species complex, F. graminearum species complex, and F. fujikuroi species complex based on mycological characteristics. Sequence typing analysis was conducted using translation elongation factor 1 alpha (TEF) to confirm the identification of isolates. All isolates were identified as F. solani, F. oxysporum, F. commune, F. asiaticum, and F. fujikuroi based on phylogenetic analysis of TEF sequences. Pathogenicity of 44 isolates was tested on three cultivars of soybean using the root dip inoculation method. Out of 5 Fusarium species, only F. asiaticum could not cause the symptoms or be weak. Ten isolates were selected based on pathogenic characters and species identification to investigate the host range and screen soybean cultivars for resistance. Fusarium solani, F. oxysporum, and F. commune were aggressive only to soybean, and F. fujikuroi was aggressive to kidney bean, yellow cowpea, black cowpea, adzuki bean as well as soybean. All 13 Korean soybean cultivars were susceptible to F. commune and F. fujikuroi. Out of 13 cultivars, cv. Janggi, cv. Poongsannamul, and cv. Socheongja were resistant to Fusarium wilt, while cv. Hwanggeumol and Chamol were susceptible to Fusarium wilt.
Keywords
Fusarium wilt; soybean; Fusarium species complex;
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1 Diaz Arias MM, Leandro LF, Munkvold GP. Aggressiveness of Fusarium species and impact of root infection on growth and yield of soybeans. Phytopathology 2013;103:822-32.   DOI
2 Chang X, Dai H, Wang D, Zhou H, He W, Fu Y, Ibrahim F, Zhou Y, Gong G, Shang J, et al. Identification of Fusarium species associated with soybean root rot in Sichuan province, China. Eur J Plant Pathol 2018;151:563-77.   DOI
3 Zhang JX, Xue AG, Cober ER, Morrison MJ, Zhang HJ, Zhang SZ, Gregorich E. Prevalence, pathogenicity and cultivar resistance of Fusarium and Rhizoctonia species causing soybean root rot. Can J Plant Sci 2013;93:221-36.   DOI
4 Korean Statistical Information Service. Statistical Database [Internet]. Daejeon: Korean Statistical Information Service; 2020 [cited 2020 Jun. 26]. Available from http://kosis.kr/index/index.do.
5 Kang IJ, Shim HK, Heu S, Kim KS. First report of soybean root and stem rot caused by Fusarium graminearum in South Korea. Plant Dis 2020;104:568.   DOI
6 Gerlach W, Nirenberg H. The genus Fusarium: A pictorial atlas. Berlin: Kommissionsverlag P. Parey; 1982.
7 Leslie JF, Summerell BA. The Fusarium laboratory manual. New York: John Wiley & Sons; 2006.
8 Choi HW, Kim JM, Hong SK, Kim WG, Chun SC, Yu SH. Mating types and optimum culture conditions for sexual state formation of Fusarium fujikuroi isolates. Mycobiology 2009;37:247-50.   DOI
9 Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-9.   DOI
10 O'Donnell K, Kistler HC, Cigelnik E, Ploetz RC. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc Natl Acad Sci USA 1998;95:2044-9.   DOI
11 Ye W, Zeng D, Xu M, Yang J, Ma J, Wang Y, Zheng X. A LAMP-assay-based specific microbiota analysis reveals community dynamics and potential interactions of 13 major soybean root pathogens. J Integr Agric 2020;19:2056-63.   DOI
12 Okello PN, Petrovic K, Singh AK, Kontz B, Mathew FM. Characterization of species of Fusarium causing root rot of soybean (Glycine max L.) in South Dakota, USA. Can J Plant Pathol 2020. https://doi.org/10.1080/07060661.2020.1746695.
13 Choi HW, Hong KS, Kim JS, Lee YK. First report of Fusarium fujikuroi causing Fusarium wilt on Glycine max in Korea. Plant Dis 2019;103:2469.
14 Farias GM, Griffin GJ. Roles of Fusarium oxysporum and Fusarium solani in Essex disease of soybean in Virginia. Plant Dis 1989;73:38-42.   DOI
15 Allen TW, Bradley CA, Sisson AJ, Byamukama E, Chilvers MI, Coker CM, Collins AA, Damicone JP, Dorrance AE, Dufault NS, et al. Soybean yield loss estimates due to diseases in the United States and Ontario, Canada, from 2010 to 2014. Plant Health Prog 2017:18:19-27.   DOI
16 Kolander TM, Bienapfl JC, Kurle JE, Malvick DK. Symptomatic and asymptomatic host range of Fusarium virguliforme, the causal agent of soybean sudden death syndrome. Plant Dis 2012;96:1148-53.   DOI
17 Bandara AY, Weerasooriya DK, Bradley CA, Allen TW, Esker PD. Dissecting the economic impact of soybean diseases in the United States over two decades. PLoS One 2020;15:e0231141. Doi:10.1371/journal.pone.0231141.   DOI
18 Moon YG, Lee JH, Choi JK, Kang AS, Han SS. Yield loss assessment and determination of economic thresholds limits against soybean anthracnose. Korean J Pestic Sci 2010;14:133-7.
19 Shim H, Lee JH, Lee YH, Myung IS, Choi HW. Establishment of economic threshold by evaluation of yield component and yield damages caused by leaf spot disease of soybean. Res Plant Dis 2013;19:196-200.   DOI
20 Hong SJ, Kim YK, Jee HJ, Shim CK, Kim MJ, Park JH, Han EJ, Lee BC. Influence of disease severity of bacterial pustule caused by Xanthomonas axonopodis pv. glycines on soybean yield. Res Plant Dis 2011;17:317-25.   DOI
21 Ko YM, Choi J, Lee YH, Kim HT. First report of charcoal rot caused by Macorphomina phaseolina on Glycine max in Korea. Res Plant Dis 2020;26:29-37.   DOI