• 대한화장품학회지
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• 제44권3호
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• pp.277-284
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• 2018

콜라겐 펩타이드(collagen peptide)는 단백질의 가수 분해물로서 주름 완화, 보습력 증대, 탄력 개선 등의 특정 피부 효능을 나타낼 수 있어 화장품 혹은 피부 개선 기능식품으로서 활용되고 있다. ${\gamma}-aminobutyric$ acid (GABA)는 척추 동물의 뇌, 척수에 존재하는 신경전달 물질로서 수면의 질과 양을 개선해 준다고 알려져 있다. 본 연구에서는 콜라겐 펩타이드와 GABA의 복합물이 수면 장애가 있는 여성에게 8주 경구 섭취를 통해서 수면 및 피부 상태를 개선할 수 있는가에 관하여 확인하였다. 복합물(J85091900)은 8주간 연속적으로 섭취 시, 수면장애지수(PSQI)가 유의적으로 감소하였으며, 수면 시간을 7% 증가시켰다. 또한, 피부 거칠기, 눈가 주름 및 피부 수분량(capacitance)을 유의적으로 개선하였다. 이상의 결과에서 콜라겐과 GABA의 복합물은 복합 수면 장애에 따른 피부의 노화 현상으로부터 피부를 보호할 수 있음을 확인하여 먹는 화장품의 핵심 소재로 활용 가능함을 확인하였다.

• BMB Reports
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• 제53권10호
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• pp.539-544
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• 2020

Skin aging appears to be the result of overlapping intrinsic (including genetic and hormonal factors) and extrinsic (external environment including chronic light exposure, chemicals, and toxins) processes. These factors cause decreases in the synthesis of collagen type I and elastin in fibroblasts and increases in the melanin in melanocytes. Collagen Type I is the most abundant type of collagen and is a major structural protein in human body tissues. In previous studies, many products containing collagen derived from land and marine animals as well as other sources have been used for a wide range of purposes in cosmetics and food. However, to our knowledge, the effects of human collagen-derived peptides on improvements in skin condition have not been investigated. Here we isolate and identify the domain of a human COL1A2-derived protein which promotes fibroblast cell proliferation and collagen type I synthesis. This human COL 1A2-derived peptide enhances wound healing and elastin production. Finally, the human collagen alpha-2 type I-derived peptide (SMM) ameliorates collagen type I synthesis, cell proliferation, cell migration, and elastin synthesis, supporting a significant anti-wrinkle effect. Collectively, these results demonstrate that human collagen alpha-2 type I-derived peptides is practically accessible in both cosmetics and food, with the goal of improving skin condition.

• 대한화장품학회지
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• 제45권3호
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• pp.299-306
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• 2019

Ginseng berry(GB)는 진세노사이드Re를 함유하고 있으며, 항염, 피부 주름 완화의 기능을 가지고 있다. 본 연구에서는 Ginseng berry 아미노산 복합체의 TLC 분획 fraction 1, 2, 4를 확인하고 HPLC로 분석하였으며, fraction 1의 LC/MASS 분석을 통해 peptide (AP-1)를 동정하였다. AP-1에 의한 NO 생성 억제 효과를 조사하여 항염증 활성을 확인하였다. 또한 procollagen type I C-peptide (PIP) ELISA kit를 이용한 collagen 합성은 대조군 대비 50% 이상의 효과를 보였다. 이상의 결과로부터 진생베리 아미노산 복합체로부터 분리한 펩타이드는 항염과 주름개선 효능을 가진다고 사료되며, 향후 항염 및 항노화 화장품 원료로서의 이용 가능성을 보였다.

• Journal of Dairy Science and Biotechnology
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• 제37권2호
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• pp.136-153
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• 2019

The present study aimed to investigate the effects of skin care foods on the synthesis of pro-collagen type I C peptide and suppression of matrix metalloproteinase (MMP)-1 secretion through an in vitro study using fibroblasts (Hs68 cells) and keratinocytes (HaCaT cells). Among the three skin care foods developed in this study, three beans cookie and avocado yoghurt influenced the production of pro-collagen type I C peptide and suppressed MMP-1 secretion; however, tiger nut Galsu drink did not exhibit these effects. All skin care foods, including three beans cookie and plain yoghurt ($50{\mu}g/mL$, p<0.001) influenced the suppression of MMP-1 in addition to other commercially available breast milk production support foods examined, such as Heath Heather ($50{\mu}g/mL$, p<0.001), Happy Mama ($50{\mu}g/mL$, p<0.01), BioLys ($50{\mu}g/mL$, p<0.001), Enfamama ($25{\mu}g/mL$, p<0.0001), and Pregnagen ($25{\mu}g/mL$, p<0.001). Avocado fruit yoghurt ($25{\mu}g/mL$, p<0.05), avocado fruit jam yoghurt ($50{\mu}g/mL$, p<0.01), Enfamama ($100{\mu}g/mL$, p<0.05), and Pregnagen ($100{\mu}g/mL$, p<0.05) influenced the production of pro-collagen type I C peptide and suppressed MMP-1 secretion. This result indicates that only avocado jam yoghurt significantly influenced both the prevention of skin keratinization and acceleration of recovery of skin fibrous structure. Therefore, avocado is a favorable ingredient for nutrition-balanced dietary foods or an essential ingredient in products for revitalization of human skin.

• 한국환경과학회지
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• 제20권8호
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• pp.1021-1030
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• 2011

The objective of this study was to assess improvements caused by chitosan, sericin and collagen peptide extract complexes (1:1:1, w/w/w, CSC-F-005) in lipid concentrations in the sera of dyslipidemic rats (SD strain) fed on experimental diets for 5 weeks. Serum concentrations of total cholesterol, HDL-cholesterol, ratio of HDL-cholesterol concentration to total cholesterol, atherosclerotic index, LDL-cholesterol, free cholesterol, cholesteryl ester, triglyceride, phospholipid and blood glucose were effective on the metabolic regulation of dyslipidemic rats. The activities of alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase in serum were remarkably lower in the extract complexes (CSC-F-005) than in the dyslipidemic model. From the above results shows that CSC-F-005 extract complexes were effective on the improvement of the lipid metabolism in sera of dyslipidemic rats.

• Journal of Microbiology and Biotechnology
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• 제31권10호
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• pp.1401-1408
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• 2021

This study examined whether the oral administration of low-molecular-weight collagen peptide (LMCP) containing 3% Gly-Pro-Hyp with >15% tripeptide (Gly-X-Y) content could ameliorate osteoarthritis (OA) progression using a rabbit anterior cruciate ligament transection (ACLT) model of induced OA and chondrocytes isolated from a patient with OA. Oral LMCP administration (100 or 200 mg/kg/day) for 12 weeks ameliorated cartilage damage and reduced the loss of proteoglycan compared to the findings in the ACLT control group, resulting in dose-dependent (p < 0.05) improvements of the OARSI score in hematoxylin & eosin (H&E) and Safranin O staining. In micro-computed tomography analysis, LMCP also significantly (p < 0.05) suppressed the deterioration of the microstructure in tibial subchondral bone during OA progression. The elevation of IL-1β and IL-6 concentrations in synovial fluid following OA induction was dose-dependently (p < 0.05) reduced by LMCP treatment. Furthermore, immunohistochemistry illustrated that LMCP significantly (p < 0.05) upregulated type II collagen and downregulated matrix metalloproteinase-13 in cartilage tissue. Consistent with the in vivo results, LMCP significantly (p < 0.05) increased the mRNA expression of COL2A1 and ACAN in chondrocytes isolated from a patient with OA regardless of the conditions for IL-1β induction. These findings suggest that LMCP has potential as a therapeutic treatment for OA that stimulates cartilage regeneration.

• 공업화학
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• 제33권6호
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• pp.581-587
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• 2022

피혁 산업은 매년 여러 유형의 유해한 피혁 폐기물을 발생시킨다. 그중에서, 셰이빙 스크랩은 크롬이 함유되어 있어 재활용하기 어렵다. 최근에 셰이빙 스크랩을 이용한 흡착제, 충전제 및 가금류 사료와 같은 다양한 부가가치 제품생산에 활용될 수 있는 많은 연구 결과들이 발표되었다. 본 연구에서는 셰이빙 스크랩으로부터 콜라겐 펩타이드를 추출하고, 이들의 물성을 개선하여 새로운 소재로 사용될 수 있는 방법을 연구하였다. 먼저, 수산화나트륨을 이용하여 셰이빙 스크랩에 포함된 크롬을 제거하였다. 그리고 가수분해물을 제조하여 농축 및 저온 결정화를 통해 정제하였다. 정제된 콜라겐 펩타이드 농축물을 스프레이 드라이어를 이용하여 분말로 제조하였다. 추출 및 정제된 콜라겐 펩타이드와 methacrylic anhyride (MAA)를 반응시켜 이중결합을 도입하였으며, ¹H 핵자기공명분광법으로 확인하였다. 다음으로, 개질화 콜라겐 펩타이드에 2-ethylhexyl acrylate (2-EHA)와 레독스 중합하여 공중합체를 만들었으며, 적외선 분광분석으로 그라프트 여부를 정성적으로 확인하였다. 결론적으로, 본 연구를 통하여 피혁 폐기물인 셰이빙 스크랩으로부터 콜라겐 펩타이드를 추출한 후 일정한 처리를 통하여 새로운 친환경 재료로 전환할 수 있음을 확인하였다.

• Journal of Periodontal and Implant Science
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• 제38권4호
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• pp.621-628
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• 2008

Purpose: Osteopontin is one of the major non-collagenous protein of hard tissue. Use of peptide domain of biologically active protein has some advantages. The objective of this experimental study is evaluation of periodontal regenerative potency of synthetic peptide gel which containing collagen binding domain of osteopontin in the degree III periodontal defect of beagle dogs. Material and Methods: Experimental degree III furcation defect was made in the mandibular third and fourth premolar of beagles. Regenerative material was applied during flap operation. 8 weeks after regenerative surgery, all animals were sacrificed and histomorphometric measurement was performed to calculate the linear percentage of the new cementum formation and the volume percentage of new bone formation. Result: The linear percent of new cementum formation was 41.6% at control group and 67.1% at test group and there was statistically significant difference. The volume percent of new bone formation was 52.1% at control group and 58.9% at test group. Conclusion: As the results of present experiment, synthetic peptide gel containing collagen binding domain of osteopontin significantly increase new bone and cementum formation in the degree III furcation defect of canine mandible.

• Advances in environmental research
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• 제11권1호
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• pp.1-16
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• 2022

Amongst 27 isolates from deteriorated leather samples, Arthrobacter creatinolyticus KP015744 zzx28 was found to be an efficient collagenase producer. Collagenase production of 13.33 µmoles/min was shown at an optimum temperature at 37℃ after 72h and at pH 7.5 by using 2 ml/dL inoculum in 10 mg/ml collagen peptide type I as a substrate. In presence of Hg²⁺, EDTA and 𝛽-mercaptoethanol the collagenase production by the isolate was strongly inhibited however Fe²⁺, Ca²⁺and DMSO enhanced production of the enzyme. Specific activity was found to be 19.46×10³ U/mg and molecular weight 66 kD by SDS PAGE. Isolate also has potential to hydrolyze keratin which is another important protein found in leather. Experimental results propose that collagenase can be effectively used as a tool for collagen and keratin rich solid waste treatment.

• 한국산학기술학회논문지
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• 제17권3호
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• pp.611-621
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• 2016

본 연구에서는 Tilapia fish에서 얻은 콜라겐 펩타이드 (TFCP)의 섭취가 UVB를 조사한 무모생쥐에서 어떠한 효과를 나타내는지 확인하고자 하였다. 무모생쥐에 1주일에 3번 UVB를 조사하여 광노화를 유도하였고, TFCP 545, 1090 mg/kg을 매일 총 12주간 경구투여 후, 주름생성, 피부 두께, 홍반, 피부 수분량, 하이드록시프롤린, MMPs 및 filaggrin의 발현량을 측정하였다. UVB만 조사 한 군과 비교하여 UVB를 조사하고 콜라겐 펩타이드를 섭취시킨 군에서 주름생성, 피부 두께 및 홍반이 감소하였고 피부 수분량은 증가하였다. 또한, 콜라젠 양과 하이드록시프롤린의 양도 TFCP를 섭취한 군에서 UVB 조사군에 비해 유의하게 회복되는 것을 확인하였다. TFCP를 섭취한 군에서 자외선에 의해 활성화되는 MMP-2, MMP-9 뿐만 아니라 MMP-3, MMP-13의 mRNA 발현량이 억제됨을 확인하였고, filaggrin의 단백질 발현량도 증가하였다. 이러한 결과들을 통하여 TFCP의 섭취는 자외선에 의한 주름 생성을 억제하고 피부 손상을 회복시킨다는 것을 확인하였다. 따라서, TFCP는 피부미용식품 소재로서 활용 가능성이 있음을 알 수 있다.