• Korean Journal of Plant Taxonomy
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• v.39 no.4
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• pp.292-295
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• 2009

Here, we report a previously unrecorded species, Sin-an-sae-u-nan-cho (Calanthe aristulifera Rchb. f.). It was discovered on Heuksan Island in Jeollanam-do. Its natural habitat was a subtropical forest. The species can easily be characterized by the simple trichomes on the back of its leaves and spur. This species is important because new horticultural races of Calanthe can be developed by cross-breeding with other species of Calanthe.

• Korean Journal of Plant Taxonomy
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• v.40 no.3
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• pp.183-185
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• 2010

We report a unrecorded species, Da-do-sae-u-nan-cho (Calanthe ${\times}$ kibanakirishima F. Maek). It was discovered on Heuksan Island in Jeollanam Province. The species differs from C. aristulifera in the corolla color and the short, wide spur. It is also easily distinguished from the other species of Calanthe by the simple trichomes on the abaxial surface of its leaves and spur.

• Korean Journal of Plant Resources
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• v.23 no.6
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• pp.541-549
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• 2010

The present study researched morphological characteristics and analyzed the genetic diversity by using RAPD in Calanthe species, native plant in Jeju, Korea. Twenty-six samples were selected by flower color, and 19 horticultural traits were investigated to study morphological characteristics. The C. discolor had the smallest leaf, the length and width of dorsal sepal, lateral sepal, petal, central lip, lateral lip, and flower stalk length were shortest and/or smallest except the spur and ovary length in Calanthe species, but those of Calanthe discolor for. sieboldii (Dence.) Ohwi (Calanthe discolor for. sieboldii) were the largest and/or biggest, and those of variants were the intermediate between C. discolor and C. discolor for. sieboldii, but spur length was the longest in C. discolor, the shortest in C. discolor for. sieboldii, and intermediate in the variants. Ovary length in C. discolor was shortest and C. discolor for. sieboldii and variants were similar with each other. The flower colors of C. discolor were brownish red, the value of CIE Lab was between 40 and 50. The flower color of C. discolor for. sieboldii was yellowish; the value of CIE Lab was between 110 and 130. And variants had various colors between 50 and 70 in the value of CIE Lab. After analyzing multiple band patterns of PCR products, 154 bands were selected as polymorphic RAPD markers. The analysis of Genetic distance of Calanthe species using RAPD showed that C. discolor and C. discolor for. sieboldii are more distant from each other than variants, and demonstrated the fact that genetic position of variants is between the other two species.

• Horticultural Science & Technology
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• v.17 no.4
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• pp.497-499
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• 1999

Distribution analysis on natural habitats for the genus Calanthe in Mt. Halla of Cheju province revealed that C. discolor and C. sieboldii were found mostly in the range of 120~400 m and 300~600 m above the sea level, respectively. C. bicolor was found in 300~400 m where both C. discolor and C. sieboldii were overlapped. The present morphological assessments showed that C. discolor had the smallest sizes of leaves and floral parts, C. had the largest, while C. bicolor had the intermediate between the above mentioned two species. Flower colors of C. discolor were brownish red and simple white, and that of C. sieboldii was merely golden yellow, whereas C. bicolor had a diverse mixed colors originated from the other two species. The specific distribution of natural habitats and morphological characteristics of three Calanthe species led the suggestion that C. bicolor can be regarded as new species derived originally from natural hybridization between C. discolor and C. sieboldii.

• Korean Journal of Plant Taxonomy
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• v.42 no.3
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• pp.215-221
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• 2012

Five Korean Calanthe species, C. discolor, C. bicolor, C. sieboldii, C. reflexa, and C. aristulifera, were studied using amplified fragment length polymorphism (AFLP) to assess their taxonomic and genetic relationships. Sixteen accessions belonging to five native Calanthe spp. and mutants with yellow tepal and white lip (YW mutants) were studied. We identified 50 putative markers using AFLP analysis. The results of AMOVA showed that genetic variance was higher between species than within species. Genetic dissimilarity when compared with the rest of the species was the lowest for individuals of the YW mutants and the highest for individuals of C. reflexa. The mutants clustered outside the major group. Calanthe bicolor clustered with C. discolor, suggesting that its genetic composition is closer to that of C. discolor. Though it is suggested to have originated as a result of natural hybridization between C. sieboldii and C. discolor, introgression is likely to have occurred in the direction of C. discolor based on the data of molecular marker, clustering and genetic dissimilarity. Calanthe reflexa and C. aristulifera were genetically the most diverse of the species studied. In conclusion, the results showed that there is genetic diversity in Korean Calanthe species, that C. bicolor introgressed in the direction of C. discolor and that the YW mutants are genetically closer to C. sieboldii.

• Horticultural Science & Technology
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• v.17 no.4
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• pp.494-496
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• 1999

Natural habitats for Calanthe species were generally established in forests of deciduous broad-leaved trees. The habitat areas had lower summer temperatures, more precipitation, and harrow humidity ranging 75~90% throughout the year than adjacent non-habitat areas. The most influential factor on the habitat establishment was light intensity. The light levels in the habitats are cut down by tree canopies, which also reduce temperature before the defoliation in autumn. The most frequent distribution of the habitat was in the areas with light intensity of 400~1,500 lux under tree canopies and of 30,000~50,000 lux in winter after defoliation. Calanthe was characteristically distributed in the broad-leaved forests where high levels of light are reduced in summer, and cold temperatures in winter are insulated by mulched layers of fallen leaves.

• Current Research on Agriculture and Life Sciences
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• v.15
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• pp.47-52
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• 1997

The experiment was carried out to find the optimal condition for seed germination in vitro of Calanthe species. Seeds of Calanthe spp. after 90 days self pollination were well germinated in the MS medium. Germination condition of cross seeds between C. discolor and C. striata was much improved by pretreatment of micro sonic waves for 10 min. The days of germination was more shortened. and protocorm growth was more promoted in microsonic wave treatment than in non-treatment.

• The Korean Journal of Mycology
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• v.46 no.4
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• pp.427-435
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• 2018

In this study, endophytic fungi were isolated from the surface-sterilized roots of Calanthe discolor and Cephalanthera longibracteata collected from the Chungnam, Jeju, Kyungnam and Chungbuk provinces in Korea. The morphological characteristics of the obtained isolates were examined and their sequences of the internal transcribed spacer (ITS) rDNA region were analyzed using the ITS1F and ITS4 primers for species identification. Leptodontidium orchidcola showed the highest species abundance and frequency among the isolated endophytic fungi. Additionally, the community analysis revealed a high specificity between the host plants and the endophytic fungal species.

• Current Research on Agriculture and Life Sciences
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• v.15
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• pp.17-24
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• 1997

According to the morphological characteristics of leaves, and flowers, including leaf and flower color, 24 horticultural cultivars of Calanthe discolor and C. striata which were collected from Jaeju island of Korea and cultivated at similar environmental conditions in Taegu from 1995 to 1996 were classified newly. Variation ranges were relatively narrow flower shape and leaf. but wide in other characteristics among cultivars.

• Korean Journal of Plant Resources
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• v.21 no.4
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• pp.341-345
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• 2008

Two orchid species of Calanthe discolor Lindl. and C. discolor for. sieboldii (Decne.) Ohwi, which have different form flower color and size. They were crossed in mid April by artificial pollination, and the F1 hybrid seeds were collected mid October. Germination of seeds was investigated on pre-treatment of seeds and under the various environmental conditions. Germination was promoted by moisture absorption and ultrasonic treatment of seeds. Dark culture of F1 hybrid seeds enhanced germination and protocorm formation, and development into seedlings compared with light culture. Although, plant growth regulators such as NAA and BA had a slightly promotive effect on seed germination and protocorm growth, regenerated seeding were showed abnormal growth patterns. Regenerated F1 hybrid plantlets were successfully transferred to pot.