The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Diversity of Endophytic Fungi Associated with Roots of Calanthe discolor and Cephalanthera longibracteata in Korea

새우난초와 은대난초 뿌리에서 분리한 내생균의 다양성

  • Lee, Bong-Hyung (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 이봉형 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2018.11.01
  • Accepted : 2018.11.22
  • Published : 2018.12.01
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Abstract

In this study, endophytic fungi were isolated from the surface-sterilized roots of Calanthe discolor and Cephalanthera longibracteata collected from the Chungnam, Jeju, Kyungnam and Chungbuk provinces in Korea. The morphological characteristics of the obtained isolates were examined and their sequences of the internal transcribed spacer (ITS) rDNA region were analyzed using the ITS1F and ITS4 primers for species identification. Leptodontidium orchidcola showed the highest species abundance and frequency among the isolated endophytic fungi. Additionally, the community analysis revealed a high specificity between the host plants and the endophytic fungal species.

본 연구에서는 충남, 제주, 경남, 충북 등에서 새우난초(Calanthe discolor)와 은대난초(Cephalanthera longibracteata)의 뿌리를 채취하여 표면살균한 후 내생균을 순수분리 하였다. 분리된 내생균의 형태학 특징을 관찰하고 internaltranscribed spacer (ITS) rDNA영역의 염기서열을 ITS1F와 ITS4프라이머를 이용하여 분석하였다. 서로 다른 지역에서 채집한 새우난초와 은대난초이지만, 공통적으로 Leptodontidium orchidcola가 높은 상대수도와 빈도를 나타냈으며, 내생균류의 군집차이를 분석할 결과, 지역적 차이보다는 숙주에 의한 차이가 더 큰 영향을 미치는 것으로 나타났다.

Keywords

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Fig. 1. Neighbor-joining phylogenetic tree of orchid endophytic fungi isolated from Calanthe discolor roots. Exidia uvapsassa was used as an outgroup.

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Fig. 2. Neighbor-joining phylogenetic tree of orchid endophytic fungi isolated from Cephalanthera longibracteata roots. Exidia uvapsassa was used as an outgroup.

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Fig. 3. PCA analysis of endophytic fungi communities isolated roots of Calanthe discolor and Cephalanthera longibracteata. Triangles represent fungal communities associated with the roots of the two orchid species analyzed in this study. A, C. discolor ; B, C. longibracteata.

Table 1. Relative abundances and relative frequency for endophytic fungi isolated from roots of Calanthe discolor

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Table 2. Relative abundances and relative frequency for endophytic fungi isolated from roots of Cephalanthera longibracteata

GNHHDL_2018_v46n4_427_t0002.png 이미지

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