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http://dx.doi.org/10.11110/kjpt.2012.42.3.215

Taxonomy of Korean Calanthe species and few of its mutants based on AFLP data  

Srikanth, Krishnamoorthy (Division of Science Education, Chonbuk National University)
Koo, Ja Choon (The Institute of Science Education, Chonbuk National University)
Ku, Jajung (National Arboretum)
Choi, Kyung (National Arboretum)
Park, Kwang-Woo (National Arboretum)
So, Soonku (Department of Biological Sciences, Chonbuk National University)
Choi, Yong-Gook (Division of Science Education, Chonbuk National University)
Whang, Sung Soo (The Institute of Science Education, Chonbuk National University)
Publication Information
Korean Journal of Plant Taxonomy / v.42, no.3, 2012 , pp. 215-221 More about this Journal
Abstract
Five Korean Calanthe species, C. discolor, C. bicolor, C. sieboldii, C. reflexa, and C. aristulifera, were studied using amplified fragment length polymorphism (AFLP) to assess their taxonomic and genetic relationships. Sixteen accessions belonging to five native Calanthe spp. and mutants with yellow tepal and white lip (YW mutants) were studied. We identified 50 putative markers using AFLP analysis. The results of AMOVA showed that genetic variance was higher between species than within species. Genetic dissimilarity when compared with the rest of the species was the lowest for individuals of the YW mutants and the highest for individuals of C. reflexa. The mutants clustered outside the major group. Calanthe bicolor clustered with C. discolor, suggesting that its genetic composition is closer to that of C. discolor. Though it is suggested to have originated as a result of natural hybridization between C. sieboldii and C. discolor, introgression is likely to have occurred in the direction of C. discolor based on the data of molecular marker, clustering and genetic dissimilarity. Calanthe reflexa and C. aristulifera were genetically the most diverse of the species studied. In conclusion, the results showed that there is genetic diversity in Korean Calanthe species, that C. bicolor introgressed in the direction of C. discolor and that the YW mutants are genetically closer to C. sieboldii.
Keywords
Korean Calanthe; molecular marker; genetic distance; UPGMA;
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