Browse > Article

Aseptic Germination of F1 Hybrid Seed by Inter-species Pollination of Calanthe discolor Lindl. and C. discolor for. Sieboldii (Decne.) Ohwi  

Kim, Kwang-Soo (Mokpo Experiment Station, National Institute of Crop Science, RDA)
Kim, Jong-Sun (Green 2000)
Park, Jong-Hwan (Green 2000)
Publication Information
Korean Journal of Plant Resources / v.21, no.4, 2008 , pp. 341-345 More about this Journal
Abstract
Two orchid species of Calanthe discolor Lindl. and C. discolor for. sieboldii (Decne.) Ohwi, which have different form flower color and size. They were crossed in mid April by artificial pollination, and the F1 hybrid seeds were collected mid October. Germination of seeds was investigated on pre-treatment of seeds and under the various environmental conditions. Germination was promoted by moisture absorption and ultrasonic treatment of seeds. Dark culture of F1 hybrid seeds enhanced germination and protocorm formation, and development into seedlings compared with light culture. Although, plant growth regulators such as NAA and BA had a slightly promotive effect on seed germination and protocorm growth, regenerated seeding were showed abnormal growth patterns. Regenerated F1 hybrid plantlets were successfully transferred to pot.
Keywords
Calanthe discolor Lindl.; C. discolor for. sieboldii (Decne.) ohwi; Pre-treatment; Moisture absorption; Plant growth regulator;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kim, Y.S. and S.H. Kim. 1989. A taxonomic study on Calanthe in Korea. Kor. J. Plant Tax. 19: 273-287(in Korean)   DOI
2 Mathews, V.H. and P.S. Rao. 1980. In vitro multiplications of Vanda hybrids through tissue culture technique. Plant Sci. Lett. 17: 383-389   DOI   ScienceOn
3 Miyoshi, K. and M. Mii. 1988. Ultrasonic treatment for enhancing seed germination of terrestrial orchid, Calanthe discolor, in asymbiotic culture. Scientia Horticulturae 35: 127-130   DOI   ScienceOn
4 Pierik, R.L.M., P.A. Spernkels, B. Van Der Harst and Q.N. Van Der Meys. 1988. Seed germination and further development of plantlets of Paphiopedilum Pfitz. in vitro. Scientia Horticulture 34: 139-153   DOI   ScienceOn
5 이창복. 1993. 대한식물도감. 향문사. 서울특별시. pp. 810
6 Stimart, D.P. and P.D. Ascher. 1981. In vitro germination of Paphiopedilum seed on a completely defined medium. Scientia Horticulturae 14: 165-170   DOI   ScienceOn
7 Hyun, M.R., J.Y. Choi, J.N. Suh, I.S. So and J.S. Lee. 1999b. Isozyme and Randomly Amplified Polymorphic DNA (RAPD) Analysis for Genetic Relationship among Calanthe discolor, C. sieboldii, and C. bicolor Native to Cheju Island. Kor. J. Hort. Sci. Technol.17: 141-143(in Korean)
8 Park, S.Y., H.N. Murthy and K.Y. Paek. 2000. In vitro Seed Germination of Calanthe sieboldii, an Endangered Orchid Species. J. Plant Biology 43: 158-161   DOI   ScienceOn
9 Miyoshi, K. and M. Mii. 1995. Phytohormone pre-treatment for the enhancement of seed germination and protocorm formation by the terrestrial orchid, Calanthe discolor (Orchidaceae), in asymbiotic culture. Scientia Horticulture 63: 263-267   DOI   ScienceOn
10 장길훈. 2006. 새우난. 월간 난과 생활. 서울특별시. pp. 238-255
11 Kim, B.C., M.H. Kim and M.Y. Oh. 1990. A taxonomic study on Calanthe in Cheju island - A comparative study on isozyme by electrophoresis. Kor. J. Plant Tax. 20: 53-64(in Korean)   DOI
12 Chung, M.Y., J.D. Chung and S.O. Jee. 1998. Effect of culture media on asymbiotic seed germination and those seedling growth of Calanthe discolor and Habenaria radiata. Korean J. Plant Tiss Cult. 25: 189-194
13 Kim, C.H. and J.D. Chung. 1997. Asymbiotic germination of Korean native Calanthe species. Agric. Res. Bull. Kyungpook Natl. Univ. 15: 47-52(in Korean)
14 Hyun, M.R., J.Y. Choi, J.N. Suh, I.S. So and J.S. Lee. 1999a. Studies on distributions and morphological characteristics of Calanthe discolor, C. sieboldii, and C. bicolor native to Cheju Province. Kor. J. Hort. Sci. Technol.17: 498-500(in Korean)