Hong, Yong Hee;Ha, Sun Mi;Jeon, You Hoon;Yang, Hyeon Jong;Pyun, Bok Yang
Clinical and Experimental Pediatrics
/
v.51
no.5
/
pp.506-511
/
2008
Purpose : The results of pulmonary function test (PFT) in children are variable according to the patient's cooperation and comprehensiveness. This study has intended to figure out the effectiveness of pre-education and training with balloons on PFT in children. Methods : One hundred six children mean aged $9.35{\pm}2.92\;years$ were tested. All participants performed PFT twice in 30 minutes intervals. First PFT were performed after usual instruction and second PFT were performed according to randomly classified grouping; Group 1 : repeat PFT after training with balloons, Group 2 : repeat PFT after training and education, both, Group 3 : repeat PFT after education about objects and necessities of pulmonary function test, Group 4 : repeat PFT without any education and training. Results : There were no difference statistically on the results of percent of predicted $FEV_1$, $FEV_1/FVC$, $FEF_{25-75%}$ and PEFR in Group 1, 2 and 4 patients. In some cases, the average is decreased with repeated PFT. At the case of repeated PFT after education, the average of percent of predicted $FEV_1$, $FEV_1/FVC$, $FEF_{25-75%}$ and PEFR is increased. There was statistically significant difference on the value of $FEV_1$, $FEF_{25-75%}$ and PEFR between first and second PFT in Group 3. Conclusion : Training with balloons before PFT causes fatigueness and lowers concentration. Understanding of PFT makes results better than training. Therefore, enough explanation and education about PFT before examination is most effective for appropriate PFT in children.
Phonsuwan, Malinee;Lee, Min Ho;Moon, Byeong Eun;Kim, Young Bok;Kaewjampa, Naruemol;Yoon, Yong Cheol;Kim, Hyeon Tae
Journal of Bio-Environment Control
/
v.25
no.4
/
pp.343-350
/
2016
The aim of this study was to evaluate effects of immature compost on the amount of nutrient content, heavy metal concentration, and application rate that were used for lettuce cultivation. The characteristics of the two composts (Compost A (CA) was immature compost and Compost B (CB) was mature compost) were evaluated upon mixing with commercial soil at 0%, 25%, 50%, and 75% (w/w). The poor chemical characteristics were appeared by use of immature compost as soil amendment; the 50% and 75% rates were weakly acidic at pH 5.39 and 5.50, respectively. The total carbon content at using of 75% of the immature compost and mature compost increased the most to 14.5 and 6.5% and it significantly increased concentrations of the total nitrogen and phosphorus compared to control. As for 75% mature compost rate increased significantly the concentrations of Cu ($128mg\;kg^{-1}$), Zn ($260mg\;kg^{-1}$), Pb ($0.32mg\;kg^{-1}$) and, Cd ($0.48mg\;kg^{-1}$) compared to control, and the highest As concentration increased significantly at 75% and 50% (6.69 and $6.28mg\;kg^{-1}$) including in 25% immature compost as $6.48mg\;kg^{-1}$. However, all of the high compost rates significantly decreased the shoot biomass of lettuce. The immature compost was potentially amended at an application rate of 25% due to a slight salinity and low risk to heavy metal uptake on lettuce growth. This use may be available if the rate is lower than that used in this trial.
Lee, Eun Mo;Park, Sang Kyu;Kim, Gyoung Je;Lee, Bong Chun;Lee, Hee Chul;Yun, Yeo Uk;Park, Soo Bok;Choi, Jong Myoung
Journal of Bio-Environment Control
/
v.26
no.4
/
pp.361-367
/
2017
Production cost as well as environmental contamination can be reduced by reuse of drained nutrient solution in hydroponic. This research was conducted to obtain the information in changes in inorganic elements concentration of supplied and drained nutrient solution as well as of plant leaves. To achieve the objective, the samples of supplied and drained solution and cherry tomato leaf tissues were periodically collected and analyzed during the hydroponic cultivation. The electrical conductivity (EC) of supplied and drained nutrient solution in early growth stage of cherry tomato were measured as around $2.0dS{\cdot}m^{-1}$, but those values move up with the passage of time reaching to $2.0dS{\cdot}m^{-1}$ at flowering stage of 9th fruiting node. The pHs of drained solution in early growth stage were 6.4 to 6.7, however those showed a tendency to get lowered to 5.9 to 6.1 as time passed during the crop cultivation. The concentration differences of $NO_3-N$, P, K, Ca, and Mg between supplied and drained solution were not distinctive until flowering stages of 4th fruiting nodes, while those in drained solution moved up after the stage. The tissue N contents of leaves decrease gradually and those of K and Ca increased as crops grew. However, Tissue P and Mg contents were maintained similarly from transplant to end-crop. The above results would be used in correction of drained nutrient solution when element compositions are varied compared to supplied solution in hydroponic cultivation of tomatoes.
Kim, Won Hyo;Kim, In Soo;Kong, Chang gi;Han, Jae Bok
Journal of the Korean Society of Radiology
/
v.14
no.3
/
pp.319-336
/
2020
The purpose of this study was to compare the outcomes of two interventional methods, overlapping drug-eluting stents (DES) and long DES, for long-term clinical outcomes in patients with acute myocardial infarction (AMI). A total of 438 patients with AMI (65.9±11.0 years, 306 males) from June 2008 to March 2019 who had diffuse long coronary artery lesion, more than 30mm were divided into two groups; group I (overlapped DES group; n=140) and group II (long DES group; n=298). We compared the incidences of major adverse cardiac events [MACEs; cardiac death, myocardial infaction (MI), target lesion revascularization (TLR) and stent thrombosis (ST)] during 12 months between the two groups. Everolimus-eluting stent was more commonly used in group II than in group I (28.1% vs. 51.8% p<0.001). Mean lesion diameter was slightly longer in group II (3.1±0.3mm vs. 3.2±0.3mm, p=0.042), and prevalence of ACC/AHA lesion type C was higher in group I (41.7% vs. 25.4%, p<0.001). Incidences of MACEs during 12 months were higher in group I than group II (18.5% vs. 14.4%, p=0.034). The rates of cardiac death (2.1% vs. 4.4%, p=0.667), MI (5.0% vs. 2.7%, p=0.260) and stent thrombosis rate (0.7% vs. 1.7%, p=0.669) were similar between the two groups. However, TLR rate was higher in group I (10.7% vs. 5.6%, p=0.041). In multivariate logistic regression analysis, presence of diabetes mellitus [Hazard ratio (HR) 2.383, 95% confidence interval (CI) 1.332-4.260, p=0.003] and use of paclitaxel-eluting stent (HR) 2.367, 95% CI 1.371-4.086, p=0.002) were independent predictors of 12-month MACEs, without significant differences in prevalence between the two groups. In AMI patients with diffuse long lesion, TLR rate was higher in the overlapped DES group during 12-month follow-up. Presence of diabetes and use of paclitaxel-eluting stent were independent predictors of MACEs.
Difractose anhydrides (DFAs) is studied as a sweetener for diabetics because of its structural property. DFAs have four types: DFA I, III, IV (degradation of levan) and V (degradation of inulin). Especially, DFA IV has been shown to enhance the absorption of calcium in experiments using rats. Levan fructotransferase is an enzyme for producing di-d-fructose-2,6':6,2-dianhydride (DFA IV). To identify structural characterization, we purified wild-type and mutants (D63A, D195N and N85S) of levan fructotransferase (LFTase) from Microbacterium sp. AL-210. These proteins were purified to apparent homogeneity by Ni-NTA affinity column, Q-sepharose ion exchange and gel filtration chromatography and detected by SDS-PAGE. They were also analyzed by circular dichroism (CD) measurements, JNET secondary structure prediction, activity measurements at various temperatures, and pH analysis. The optimum pH for the enzyme-catalyzed reaction was pH 7.5 and optimum temperature was observed at $55^{\circ}C$. Along with wild-type LFTase, mutants were analyzed by CD measurement, fluorescence analysis and differential scanning calorimetry (DSC). N85S showed less $\alpha$-helix and more $\beta$ strand than others. Also, N85S showed almost the same curve as wild-type in their steady-state fluorescence spectra, whereas mutant D63A and D195N showed higher intensity than wild-type. The amino acid sequence of wild-type LFTase was compared to the sequences of exo-inulinase from Aspergillus awamori, a plant fructan 1-exohydrolase from Cichorium intybus, and Thermotogo maritime (Tm) invertase and showed a high identity with Exo-inulinase from Aspergillus awamori.
Park, Hee-Bok;Han, Sang-Hyun;Kang, Yong-Jun;Shin, Moon-Cheol;Lee, Jae-Bong;Cho, In-Cheol
Journal of Life Science
/
v.27
no.3
/
pp.295-300
/
2017
This study examined the association between genotypes of the isocitrate dehydrogenase 3, beta subunit (IDH3B) gene and economic traits in an $F_2$ population of Duroc and Jeju (South Korea) native black pigs (JBPs). The genotypes was determined the presence/absence of a 304-bp insertion/deletion fragment in the promoter region of the IDH3B gene for JBP, Duroc, and their $F_1$ and $F_2$ progeny. Three genotypes (AA, AB and BB) were found in the $F_1$ and $F_2$ populations, but there was no AA genotype found in JBP and no BB in Duroc. Association analysis results showed the significant differences with carcass weights (CW), backfat thicknesses (BFT) and eye muscle area (EMA) (p<0.05), but not with growth traits including body weights and average daily gains at different stages, reproductive traits including teat numbers, and crude fat contents (CFAT) measured in longissimus dorsi (p>0.05). The $F_2$ pigs possessing the IDH3B BB homozygote had heavier CW ($72.92{\pm}11.133kg$), thicker BFT ($25.75{\pm}6.06mm$), and larger EMA ($23.82{\pm}4.825cm^2$) than those from the other genotypes (p<0.05). These results were estimated that there are biological roles related with IDH3B genotypes resulting development of EMA, BFT, and CW but not with intramuscular fat deposition during late period of pig production. Our findings suggest that the 304-bp insertion allele of porcine IDH3B may be a genetic marker for marker assistant selection for improving meat productivity of the Jeju Black pig and Duroc-related molecular breeding systems.
Lee, Ga-Young;Kim, Min Jee;Kim, So Yeon;Lee, Kyung Bok;Oh, Dong Hyun;Cho, Young Ho;Yoo, Yung Choon
Journal of Life Science
/
v.30
no.10
/
pp.905-911
/
2020
The adjuvant effect of PAMAM dendrimer G4 (PAMAM) on the induction of humoral and cellular immune responses against keyhole limpet hemocyanin (KLH) was examined. Mice were immunized subcutaneously twice at two-week intervals with KLH, with or without PAMAM dendrimer (100 ㎍/mouse), and the mice immunized with KLH+PAMAM showed significantly higher antibody titers against KLH than those immunized with KLH alone. The assay for determining the isotypes of the antibodies showed that PAMAM augmented the KLH-specific antibody titers of IgG1, IgG2a, IgG2b, IgG3, and IgM. In addition, mice immunized twice with KLH+PAMAM followed by a subcutaneous injection of KLH (20 ㎍/site) 7 weeks after the primary immunization exhibited a higher delayed-type hypersensitivity (DTH) reaction than those treated with KLH alone. In an in vitro analysis of T lymphocyte proliferation in response to KLH in week 8, the splenocytes of mice treated with KLH+PAMAM showed significantly higher proliferating activity than those treated with KLH alone, and the culture supernatants of cell cultures from mice immunized with added PAMAM dendrimer showed higher levels of KLH-specific cytokine (IL-4 and IFN-r) production. These results suggest that PAMAM dendrimer G4 possesses a potent immune-adjuvant activity for enhancing both humoral and cell-mediated immunity specific to foreign antigens.
Kim, Chang Hwi;Pyun, Bok Yang;Hong, Young Jin;Kang, Jin Han
Pediatric Infection and Vaccine
/
v.7
no.1
/
pp.120-128
/
2000
Purpose : Active immunization against hepatitis A with an inactivated vaccine reveals excellent immunogenicity, tolerability and protective efficacy. Inactivated hepatitis A vaccines have been selectively used since 1996 in Korea to prevent hepatitis A. This study was performed to assess the immunogenicity and reactogenicity after two doses of HM175 strain hepatitis A vaccine in healthy Korean children. Methods : 128 healthy children(M/F; 65/63) aged 1 to 15 years, who were seronegative for hepaitatis A, participated in this study. A alum-adsorbed vaccine containing 720 EL.U of antigen form HM175 hepatitis A strain per 0.5 mL dose was injected intramuscularly on the deltoid area. The second dose was given 6 months later, Anti-HAV antibodies were measured by ELISA before and 1 month after each vaccination to assess the immunogenicity. Any local and general adverse events were reported by patients parents with the prepared questionnaire after each vaccination. Results : 120 volunteers(M/F; 60/60) completed the whole series of the study. Seroconversion occurred in all cases after primary and booster vaccination. The mean anti-HAV antibody titer after primary vaccination was 389.2mIU/mL, and 3,609mIU/mL after booster vaccination. And levels of anti-HAV antibodies after booster immunization were significantly higher in female children. The most common local adverse event was soreness on the injection site, but it was mild and resolves within 3 days. Fever was not reported after booster vaccination. Conclusion : Based on these data, we conclude that the inactivated HM175 strain hepatitis A vaccine is highly immunogenic and tolerable in healthy Korean children.
Kim, Young Sin;Kim, Jae-Hwan;Suh, Sang Won;Kim, Hyun;Byun, Mi-Jeong;Kim, Myung-Jick;Kim, Ji Sung;Lee, Ji Woong;Choi, Seong-Bok
Korean Journal of Poultry Science
/
v.39
no.4
/
pp.283-290
/
2012
The objective of this study was to compare the growth performance between Korean native layer chickens and imported layer chickens at early rearing stage. Total number of chicks analyzed in this study was 276 and feeding period was conducted from July 24, 2012 for 10 weeks. Five strains including 2 Korean native strains: A=Korean Native Black (Chungcheongbuk-do) and B=Korean Native Yellowish Brown (Gyeongsangbuk-do) and 3 imported layer strains: C=White Leghorn (Gyeongsangnam-do), D=White Leghorn (Seoul), and E=Ameraucanas (Gyeongsangbuk-do) were used to analyze the following traits such as fertility, hatchability, body weight at a different growing stage, average body weight gain, and feed conversion ratio. The fertilities and hatchabilities of strains were 93.88% and 95.65% in strain A, 81.75% and 86.24% in strain B, 82.25% and 88.15% in strain C, 79.25% and 90.85% in strain D, and 71.50% and 88.11% in strain E, respectively. A viability was excellent in strains A and E to be more than 98% and was low in strain D to be 86.67% at a whole week. The strain A had greater body weight during growing stages (p<0.05) than the other strains. The shank length of strain D of $56.69{\pm}3.27mm$ was the highest value at 10 weeks of age among strains (p<0.05). The phenotypic correlation coefficients of strains A and D between an average body weight gain and a shank length were 0.63 and 0.73 during 0~2 wk, 0.70 and 0.55 during 2~4 wk, 0.55 and 0.54 during 4~6 wk, 0.50 and 0.24 during 6~8 wk, and 0.46 and 0.29 during 8~10 wk, respectively. The Korean native hens may have potential abilities to be used as an excellent seed stock for poultry industry.
The purpose of this survey was to collect basic data on breeding systems of animal welfare-approved laying hen farms in Korea. Questionnaires were mailed to 64 animal welfare-approved farms, and 20 questionnaires (31.3%) were returned. The housing systems were fabric coverlet (4 farms, representing 20%), naturally farmed (Yamagisi, 7 farms, 35%), and steel panel-framed housing (9 farms, 45%). The 20 farms had stocking densities of $2{\sim}3birds/m^2$ (2 farms; 10%), $4{\sim}5birds/m^2$ (10 farms; 50%), and $6{\sim}7birds/m^2$ (8 farms; 40%). Breeding methods were floor-housed (14 farms; 70%), free-range (3 farms; 15%), and floor plus free-range (3 farms; 15%). Stocking density was $4{\sim}6birds/m^2$ at most of the farms with fabric coverlet and naturally farmed housing and $6{\sim}7birds/m^2$ at seven farms (of 9 farms) with a steel panel-framed housing. The daily feed intake of 11 farms (55%) was between 120 and 130 g, which included 3 farms (15%) with fabric coverlet, 3 farms (15%) with naturally farmed housing, and 5 farms (25%) with steel panel-framed housing. The age of peak production was 24~28 weeks overall 20 farms. Over 80% of production on fabric coverlet, naturally farmed, and steel panel-framed house farms was on 3, 4 and 6 farms, respectively. Respiratory disease on the 20 farms represented 55% of total disease incidence, and of each housing type represented 75% (fabric coverlet), 70% (naturally farmed) and 33% (steel panel-framed). E. coli disease was only found in the steel panel-framed housing. Most of the animal welfare-approved eggs were sold at large markets or a real sale markets. Egg price was 200~250 won per egg. These results indicate the current situation of animal welfare-approved farms and could be caused that windowless poultry house was applied to animal welfare approved farms.
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