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http://dx.doi.org/10.5352/JLS.2020.30.10.905

Adjuvant Effect of PAMAM Dendrimer on the Antigenicity of Keyhole Limpet Hemocyanin in Balb/c Mice  

Lee, Ga-Young (Department of Microbiology, College of Medicine, Konyang University)
Kim, Min Jee (Department of Microbiology, College of Medicine, Konyang University)
Kim, So Yeon (Department of Microbiology, College of Medicine, Konyang University)
Lee, Kyung Bok (Department of Biochemistry, College of Medicine, Konyang University)
Oh, Dong Hyun (Department of Radiology, College of Medicine, Konyang University)
Cho, Young Ho (Department of Pharmaceutics & Biotechnology, College of Medical Engineering, Konyang University)
Yoo, Yung Choon (Department of Microbiology, College of Medicine, Konyang University)
Publication Information
Journal of Life Science / v.30, no.10, 2020 , pp. 905-911 More about this Journal
Abstract
The adjuvant effect of PAMAM dendrimer G4 (PAMAM) on the induction of humoral and cellular immune responses against keyhole limpet hemocyanin (KLH) was examined. Mice were immunized subcutaneously twice at two-week intervals with KLH, with or without PAMAM dendrimer (100 ㎍/mouse), and the mice immunized with KLH+PAMAM showed significantly higher antibody titers against KLH than those immunized with KLH alone. The assay for determining the isotypes of the antibodies showed that PAMAM augmented the KLH-specific antibody titers of IgG1, IgG2a, IgG2b, IgG3, and IgM. In addition, mice immunized twice with KLH+PAMAM followed by a subcutaneous injection of KLH (20 ㎍/site) 7 weeks after the primary immunization exhibited a higher delayed-type hypersensitivity (DTH) reaction than those treated with KLH alone. In an in vitro analysis of T lymphocyte proliferation in response to KLH in week 8, the splenocytes of mice treated with KLH+PAMAM showed significantly higher proliferating activity than those treated with KLH alone, and the culture supernatants of cell cultures from mice immunized with added PAMAM dendrimer showed higher levels of KLH-specific cytokine (IL-4 and IFN-r) production. These results suggest that PAMAM dendrimer G4 possesses a potent immune-adjuvant activity for enhancing both humoral and cell-mediated immunity specific to foreign antigens.
Keywords
Adjuvant; antibody production; cellular immunity; PAMAM dendrimer; humoral immunity;
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