• Journal of Practical Agriculture & Fisheries Research
• /
• v.23 no.2
• /
• pp.5-14
• /
• 2021

It was investigated how the contents of four active ingredients, nodakenin, decursinol, decursin, and decursinol angerate, which are active ingredients of Angelica gigas Nakai, cause material changes depending on the type of bacteria. Fermentation experiments were conducted using 9 types of bacteria: 5 types of Bacillus EMD17, 9-3, HCD2, #8, 191 and 4 types of Lactobacillus KCTC 3320, WCP02, S65, P1201. 1. The contents of decursin and decursinol angerate, which are indicator substances, rapidly decreased after 2 days of fermentation by inoculating Bacillus bacteria in the extract of Angelica gigas Nakai. Even after 4 days of fermentation, the contents of decursin and decursinol angerate were the same as on the 2nd day. On the other hand, the content of nodakenin and decursinol increased after 4 days of fermentation. In addition, the content of decursin increased significantly after 6 days of fermentation. 2. Substance changes of nodakenin and decursinol after inoculation of Bacillus bacteria into the extract of Angelica gigas Nakai were almost non-existent regardless of the type of bacteria. The change in effective content of decursin and decursinol angerate was large in Bacillus EMD17 and 9-3. Changes in the contents of decursin and decursinol angerate were almost non-existent in Bacillus HCD2, #8, and 191 strains. 3. As a result of finding out the change in active ingredient after 8 days of fermentation using 4 types of Lactobacillus KCTC 3320, WCP02, S65, and P1201 extracts of Angelica gigas Nakai, there was almost no change in the contents of nodakenin and decursinol regardless of the type of bacteria. However, in the case of fermentation with Lactobacillus S65 and P1201, the contents of decursin and decursinol angerate were changed.

• Journal of Naturopathy
• /
• v.11 no.1
• /
• pp.1-8
• /
• 2022

Background: A validation study is needed to make domestic applications of German Kneipp therapy. Purpose: The study aims to test the effect of a Kneipp therapeutic program in a domestic forest environment on the autonomic nervous system. Methods: The program was made considering the 4 key elements ('exercise', 'regulative therapy', 'nutrition', and 'phytotherapy') of the Kneipp therapy. Total 3 sessions (once a week for 5 hours in each session) were performed to 40 domestic adults divided into four groups (10 in each group). HRV was measured on the subjects before and after the intervention, and its 6 characteristic parameters (TP, VLF, LF, HF, LF/HF, CSI: Cumulative Stress Index) were compared for statistical analysis. Results: For 33 subjects excluding 7 who were disturbed by interrupt factors during measurements, significant improvements after the intervention, were observed in TP(8.64%, p < ..001), VLF(6.96%, p < .05), LF(15.86%, p < .001), HF(8.46%, p < .01), LF/HF(5.77%, p < .05) and CSI(-16.06%, p < ..001). Conclusions: The Kneipp therapy performed in the forest environment was shown to activate the autonomic nervous system and in particular the sympathetic and parasympatheric nervous to promote heart activity, and was also shown to most significantly reduce cumulative stress. The present observations would be the first preliminary evidence that the Kneipp therapeutic interventation in a domestic forest environment results in positive responses of the autonomic nervous system including stress relief.

• Journal of Appropriate Technology
• /
• v.7 no.1
• /
• pp.59-71
• /
• 2021

Hybrid hydrothermal carbonization (Hybrid HTC) technology is a proprietary thermochemical process for two or more organic wastes.The reaction time is less than two hours with temperature range 180~250℃ and pressure range 20~40bar. Thanks to accumulation of the carbon of the waste during Hybrid HTC process, the energy value of the solid fuel increases significantly with comparatively low energy consumption. It has also a great volume reduction with odor removal effect so that it is evaluated as the best solid fuel conversion technology for various organic wastes. In this study of the hybrid hydrothermal carbonization, the effect on the calorific value and yield of Cambodian mango waste were evaluated according to changes in temperature and reaction time. Through the study, parameter optimization has been sought with improving energy efficiency of the whole plant. It is decomposed in the Hydro-Carbonation Technology to Generate Gas. At this time, it is possible to develop manufacturing and production technologies such as hydrogen (H₂) and methane (CH₄). Based on the results of the study, a pilot plant (2t/day) has been proposed for future commercialization purpose along cost analysis, mass balance and energy balance calculations.

• Journal of The Korean Association For Science Education
• /
• v.42 no.6
• /
• pp.567-578
• /
• 2022

In this study, we examine whether the description of the elementary science curriculum guide, the concept of evolution, and the beliefs of teachers affect the teaching practice in classes related to adaptation of elementary school teachers. First, we examined the alternative concept among the sentences described in the bio-adaptation-related unit of the 2009 Elementary Science Curriculum Teacher's Guide and identified the effects of this description on the teaching practice of elementary school teachers. Next, six elementary school teachers were classified according to the results of the evolutionary concept test paper and the evolutionary belief test paper, and based on the class recording data and interview data, whether there is a difference in teaching execution. As a result, it was confirmed that there were a total of 18 descriptions corresponding to the concept of evolutionary alternatives in the analysis of instruction descriptions, and that these descriptions influenced elementary school teachers' adaptation concepts and teaching practice. Next, the group with high and low levels of evolution differ in the areas of "recognition of importance in the unit, distinction between adaptation concepts in the general sense and adaptation concepts in the biological sense, errors in the class," and "recognition of evolutionary education needs in the elementary curriculum." This study is meaningful in that it qualitatively confirms the research on the evolution concept of elementary school teachers, which has been approached quantitatively, and in-depth, confirms how the description, evolution concepts, and evolutionary beliefs affect elementary school teachers' biological adaptation concepts.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.64 no.6
• /
• pp.87-99
• /
• 2022

The meat consumption per person has continuously increased in recent years. However, the labor force in the domestic livestock industry has decreased due to the declining and ageing population. In order to increase productivity, the government have developed and distributed design standard of livestock houses. Presently, report showed that the adaptation rate of the developed livestock house design standard on the real farm was still low. Thus, this paper aimed to find ways to improve the utilization of the design standard through surveys. The survey was conducted on 650 farms across the country. Analysis of the result showed that in the poultry house, the unawareness of farmers to the design standard was found to be the biggest reason for not using the design standards. On the other hand, in the swine house, the previously built swine houses do not fit with the design standard. From these result, the following recommendations were suggested: 1) promotion and education are needed to enhance usage of design standard; 2) since it is impossible to make a design standard considering all the farm sites, it is important to consider the conditions of various farm site prior to enhancement of the design standard; 3) improvement factors such as reinforcing the ventilation design, reflecting animal welfare, preventing livestock diseases, and enhancing ICT devices can also be promoted.

• Journal of Mushroom
• /
• v.20 no.4
• /
• pp.208-217
• /
• 2022

Mushrooms in Naejangsan National Park between May and September of 2021 have been surveyed. In this period, a total of 4 divisions, 9 classes, 25 orders, 72 families, 171 genera, and 381 species, including 3 climate-sensitive biological indicator species were found. The order in which the most diverse array of species was observed is Agaricales, which includes 24 families, 64 genera, and 170 species. Among these, the genus Russula was dominant, with 30 species, followed by the genus Amanita with 27 species. Among the 12 grids we investigated, species diversity was greatest in grid F5, in which 56 species of mushrooms were found. In particular, a large number of ectomycorrhizal mushrooms, including Russula spp. and Lactarius spp. were recognized. We presume that the gentle slopes and the low occurrence of Sasa borealis in this area may create a favorable environment for wild mushrooms. In corroboration, some grids (e.g. F6, F8, and F10) covering steep slopes and harboring large numbers of Sasa borealis contained only 19 species. Based on DNA sequence analysis, the NJ21064 was identified as Chlorophyllum hortense, which is newly recorded in Korea.

• The Korea Journal of Herbology
• /
• v.37 no.1
• /
• pp.19-29
• /
• 2022

Objectives : Although the pharmacological effects of anti-inflammatory and antioxidant action of Atractylodes macrocephala Koidzumi water extract (AM) have been proven from many studies, reports on the antioxidant effect of AM on ulcerative colitis (UC) are scarce. Therefore, we aimed at evaluating the anti-oxidant effect of AM on the DSS-induced UC model. Methods : To induce ulcerative colitis, 8-week-old male Balb/c mice received 5% DSS in drinking water for 1 week. After 1 week of adaptation, mice were divided into four groups (n=8 each) for use as normal (Normal), DSS Control (Control), DSS + AM 100 mg/kg (AM100)-treatment, DSS + AM 200 mg/kg (AM200)-treatment. After 1 week of the experiment, the animals were sacrificed, and the extracted colon tissue was analyzed for protein through western blot. Results : As a result of confirming the macroscopic changes in colon tissues to confirm the therapeutic effects of AM, the decrease in colon length was suppressed in the AM treatment group compared to the control group. In addition, as a result of biochemical analysis, AM administration significantly reduced serum glutamic oxalacetic transaminase, glutamic pyruvate transaminase levels and tissue malondialdehyde levels. As a result of confirming the protein expression level through western blot, AM administration significantly decreased the expression of NADPH-related proteins such as NOX2, p22^phox, and iNOS, but significantly increased the expression of SOD, catalase, and GPx-1/2. Conclusions : AM may improve DSS-induced UC in mice by modulating NADPH and antioxidant-related proteins. In conclusion, AM showed an antioxidant effect through the improvement of oxidative stress on UC.

• Korean journal of applied entomology
• /
• v.61 no.1
• /
• pp.249-254
• /
• 2022

Silkworms, which have for long been used as an insect resource for industrialization, have recently attracted attention as potential bio-factories for the production of novel biomaterials. In this regard, material production is typically achieved based on transformation technology, mediated via microinjection, in which a target gene is inserted into eggs containing an embryo. However, an essential step in the microinjection procedure is egg fixation, which can be a time-consuming and laborious task. Therefore, in this study, using the 3DCADian program, we adopted a 3D printing approach to model egg liners and glue drawers, which can contribute to facilitating egg alignment and fixation, thereby enhancing transformation efficiency by reducing time consumption and fatigue. After rendering using Fusion 360, the two supplementary tools were produced by printing with nylon resin (PA12) and Sinterit Lisa Pro. Subsequent analysis of the time required to fix eggs on glass slides using the two manufactured tools, revealed that the processing time was reduced by approximately 18.6% when the two tools were used compared with when these tools were not used. These innovations not only reduced fatigue but also contributed to more effective use of the microscope and manipulator for microinjection. Consequently, we believe that with additional research and refinement, the egg liner and glue drawer developed in this study could be used to enhance silkworm transformation efficiency and study similar transformation systems in other industrial insects.

• Nutrition Research and Practice
• /
• v.16 no.sup1
• /
• pp.134-146
• /
• 2022

BACKGROUND/OBJECTIVES: Accumulating evidence has shown the beneficial effects of isoflavone on health. There is limited information on the usual isoflavone intake for Koreans. This study examined the usual intake of total isoflavone and its major food sources in Koreans according to age and gender. SUBJECTS/METHODS: The dietary intake data of 21,271 participants aged 1 yrs and older from the Korea National Health and Nutrition Examination Survey (KNHANES) VII 2016-2018 were analyzed. The average isoflavone intake was estimated based on the 24-h dietary recall data in KNHANES and the isoflavone database from the Korea Rural Development Administration (RDA) and literatures. The usual isoflavone intake was estimated by applying the ratio of within- and between-participant variance estimated from the 2009 KNHANES data to the 7th KNHANES (2016-2018) data. The variance of the isoflavone intake was calculated using MIXTRAN macro with intake data for two days in the 2009 KNHANES. Complex sample analysis with stratified variables and integrated weights was conducted. RESULTS: The mean total isoflavone intake in the Korean population aged 1 yrs and older (n = 21,271) was 139.27 mg/d, which was higher than the usual intake of 47.44mg/d. Legumes were a major contributing food group (91%), with arrowroot being a major individual contributor to the isoflavone intake (67.2%), followed by 21.3% of soybean, 5.4% of bean sprouts, and 2.1% of tofu. The usual isoflavone intake was highest in the participants aged 50 to 64 yrs old and increased with age until 50 to 64 yrs and then decreased with further increases in age. The usual isoflavone intake of participants aged 65 yrs and older was higher for men than for women, showing gender differences. CONCLUSIONS: The usual dietary intake of isoflavone varied according to age and gender in the Korean population. This study showed that the usual isoflavone intake was lower than the average isoflavone intake. The difference between percentiles of the usual isoflavone intake was similarly smaller than the average intake. An estimation of average intake can be hindered by the occasional consumption of foods high in isoflavones, suggesting that the usual intake estimation method can be more appropriate. Further research will be needed to establish isoflavone dietary guidelines regarding the effects of isoflavone intake on health outcomes.

• The Journal of the Convergence on Culture Technology
• /
• v.8 no.6
• /
• pp.921-926
• /
• 2022

Sugar solutions (5%, 10%, 15% and 20%) were tested by seven sensors of Astree E-Tongue for selecting a sensor for sweetness. NMS sensor was chosen as a sensor for sweetness among two sensors (PKS and NMS sensors selected in first stage) by considering precision, linearity and accuracy. Sugar, fructose, glucose and xylitol (5%, 10% and 15%) were tested by E-tongue. The principal component analysis (PCA) result by E-Tongue with seven sensors at 5% concentration level of four sweetners was not satisfactory (Discrimination index was -0.1). On the other hand, the relative NMS sensor response values were derived as 1.08 (fructose), 0.99 (glucose) and 1.00 (xylitol) comparing to sugar. Only the E-Tongue relative glucose response 0.99 was different from 0.5~0.75 of the relative sweetness range reported as the human sensory test results. Considering the excellent precision (%RSD, 1.53~3.64%) of E-Tongue using NMS single sensor for three types of sweeteners compared to sugar in the concentration range of 5% to 15%, replacing sensory test of sweetened beverages by E-Tongue might be possible for new product development and quality control.