대한본초학회지 (The Korea Journal of Herbology)

  • 제37권1호
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  • Pages.19-29
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  • 2022
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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백출(白朮)의 항산화 효과가 DSS 유발 궤양성 대장염 모델에 미치는 영향

Antioxidant Effect of Atractylodes macrocephala Koidzumi in DSS-induced Ulcerative Colitis Model

  • 박석만 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이세희 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 정다운 (대구한의대학교 간질환한약융복합활용연구센터) ;
  • 조수정 (대구한의대학교 간질환한약융복합활용연구센터) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 박해진 (대구한의대학교 DHU 바이오융복합시험센터) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Park, Seok Man (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Se Hui (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Jeong, Da un (Research Center for Herbal Convergence on Liver Disease, Daegu Haany University) ;
  • Cho, Su-Jung (Research Center for Herbal Convergence on Liver Disease, Daegu Haany University) ;
  • Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Park, Hae-Jin (DHU Bio Convergence Testing Center, Daegu Haany University) ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 투고 : 2021.10.21
  • 심사 : 2022.01.25
  • 발행 : 2022.01.30
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초록

Objectives : Although the pharmacological effects of anti-inflammatory and antioxidant action of Atractylodes macrocephala Koidzumi water extract (AM) have been proven from many studies, reports on the antioxidant effect of AM on ulcerative colitis (UC) are scarce. Therefore, we aimed at evaluating the anti-oxidant effect of AM on the DSS-induced UC model. Methods : To induce ulcerative colitis, 8-week-old male Balb/c mice received 5% DSS in drinking water for 1 week. After 1 week of adaptation, mice were divided into four groups (n=8 each) for use as normal (Normal), DSS Control (Control), DSS + AM 100 mg/kg (AM100)-treatment, DSS + AM 200 mg/kg (AM200)-treatment. After 1 week of the experiment, the animals were sacrificed, and the extracted colon tissue was analyzed for protein through western blot. Results : As a result of confirming the macroscopic changes in colon tissues to confirm the therapeutic effects of AM, the decrease in colon length was suppressed in the AM treatment group compared to the control group. In addition, as a result of biochemical analysis, AM administration significantly reduced serum glutamic oxalacetic transaminase, glutamic pyruvate transaminase levels and tissue malondialdehyde levels. As a result of confirming the protein expression level through western blot, AM administration significantly decreased the expression of NADPH-related proteins such as NOX2, p22phox, and iNOS, but significantly increased the expression of SOD, catalase, and GPx-1/2. Conclusions : AM may improve DSS-induced UC in mice by modulating NADPH and antioxidant-related proteins. In conclusion, AM showed an antioxidant effect through the improvement of oxidative stress on UC.

키워드

과제정보

이 연구는 한국보건산업진흥원(KHIDI, HI15C00255)을 통해 보건복지부가 지원한 한약 연구개발 프로그램의 지원을 받았으며, 2021년도 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원(No. 2018R1A5A2025272)을 받아 수행된 연구이다.

참고문헌

  1. Snider AJ, Kawamori T, Bradshaw SG, Orr KA, Gilkeson GS, Hannun YA, Obeid LM. A role for sphingosine kinase 1 in dextran sulfate sodiuminduced colitis. FASEB J. 2009;23:143-152. https://doi.org/10.1096/fj.08-118109
  2. Salaritabar A, Darvishi B, Hadjiakhoondi F, Manayi A, Sureda A, Nabavi SF, Fitzpatrick LR, Nabavi SM, Bishayee A. Therapeutic potential of flavonoids in inflammatory bowel disease: A comprehensive review. World J Gastroenterol. 2017;23:5097-5114. https://doi.org/10.3748/wjg.v23.i28.5097
  3. Larussa T, Imeneo M, Luzza F. Potential role of nutraceuticalcompounds in inflammatory bowel disease. World J Gastroenterol. 2017;23:2483-2492. https://doi.org/10.3748/wjg.v23.i14.2483
  4. Stein RB, Hanauer SB. Medical therapy for inflammatory bowel disease. Gastroenterol Clin N Am. 1999;28:297-322. https://doi.org/10.1016/S0889-8553(05)70058-3
  5. Das KM, Eastwood MA, McManus JP, Sircus W. Adverse reactions during salicylazosulphapyridine therapy and the relation with drug metabolism and acetylator phenotype. N Engl J Med. 1973;289:491-495. https://doi.org/10.1056/NEJM197309062891001
  6. Kim MJ, Shin MR, Lee JA, Park SA, Park HJ, Lee JH, Roh SS. Improvement effect of Sprout of Coix lacryma-jobi var. mayuen Stapf water extract on dss-induced ulcerative colitis in mice. Kor. J. Herbol. 2020;35(6):21-28. https://doi.org/10.6116/KJH.2020.35.6.21.
  7. Shin MG. Clinical herbology. Seoul:Youngrimsa. 1992:172-173.
  8. Shou D, Dai S, Zhang J, Li H, Yu Z. [Simultaneous determination of atractylenolide III, atractylenolide I and atractylon in Artactylodis macrocephala using microbore liquid chromatography]. Se Pu. 2008;26:637-639.
  9. Li W, Wen HM, Cui XB, Zhang KW. [Process mechanismof Atractylodes macrocephala and conversion of sesquiterpenes]. Zhongguo Zhong Yao Za Zhi. 2006;31:1600-1603.
  10. Lu SY, Qian JQ, Zou XM. [Study on the extraction of the active component in Atractylode macrocephala with ultrasonic wave technology optimized by the response surface method]. Zhongguo Zhong Yao Za Zhi. 2006;31:549-552.
  11. Yosioka I, Tani T, Hirose M, Kitagawa I. Letter: Diacetylatractylodiol, a new acetylenic compound from Atractylodes japonica Koidzumi. Chemical & pharmaceutical bulletin. 1974;22:1943-1945. https://doi.org/10.1248/cpb.22.1943
  12. Ye Y, Chou GX, Wang H, Chu JH, Fong WF, Yu ZL. Effects of Sesquiterpenes Isolated From Largehead Atractylodes Rhizome on Growth, Migration, and Differentiation of B16 Melanoma Cells. Integr Cancer Ther. 2011;10(1):92-100. https://doi.org/10.1177/1534735410378660
  13. Han YK, Park YK. Effect of Atractylodis Rhizoma Alba water extract on streptozotocin-induced diabetes in rats. Kor. J. Herbol. 2011;26(4):23-30. https://doi.org/10.6116/KJH.2011.26.4.023
  14. Chun HJ, Choi EY, Yoon SC, Nam HW, Baek SH, Woo WH. Inhibitory effects of ethanol extract of Atractylodis Rhizoma alba on melanin biosynthesis. Yakhak hoeji. 2001;45(3):269-275.
  15. Jeong YH, Li W, Go Y, Oh YC. Atractylodis Rhizoma Alba attenuates Neuroinflammation in BV2 Microglia upon LPS stimulation by inducing HO-1 activity and inhibiting NF-κB and MAPK. Int J Mol Sci. 2019;20(16):4015. https://doi.org/10.3390/ijms20164015
  16. Zhou Y, Tao H, Wang A, Zhong Z, Wu X, Wang M, Bian Z, Wang S, Wang Y. Chinese herb pair Paeoniae Radix Alba and Atractylodis Macrocephalae Rhizoma suppresses LPS-induced inflammatory response through inhibiting MAPK and NF-κB pathway. Chin Med. 2019;14:2. https://doi.org/10.1186/s13020-019-0224-2
  17. Kim SH, Park YK. Effects of Atractylodis Rhizoma Alba extract on collagen-induced arthritis in mice. Kor. J. Herbol. 2012;27(3):1-6. https://doi.org/10.6116/KJH.2012.27.3.1
  18. Lee SO, Seo JH, Lee JW, Yoo MY, Kwon JW, Choi SU, Kang JS, Kwon DY, Kim YK, Kim YS, Yoo SY. Inhibitory effects of the rhizome extract of Atractylodes japonica on the proliferation of human tumor cell lines. Kor. J. Pharmacogn. 2005;36(3):201-204.
  19. Shim AR, Dong GZ, Lee HJ, Ryu JH. Atractylochromene is a repressor of Wnt/beta-Catenin signaling in colon cancer cells. Biomol Ther. 2015;23(1):26-30. https://doi.org/10.4062/biomolther.2014.095
  20. Choi EY, Oh HJ, Park NK, Chun HJ, Ahn JW, Jeon BH, Han DS, Lee HO, Baek SH. Screening of cytotoxicity and antimicrobial effects of extracts from Atractylodes macrocephala Koidz. Korean J. Oriental Physiology & Pathology. 2002;16(2):348-352.
  21. Cha, HY, Jeong AR, Cheon JH, Ahn SH, Park SY, Kim KB. The Anti-oxidative and Anti-inflammatory Effect of Lonicera Japonica on Ulcerative Colitis Induced by Dextran Sulfate Sodium in Mice. J Pediatr Korean Med. 2015;29(3):54-64. https://doi.org/10.7778/JPKM.2015.29.3.054
  22. Rama P, Vignesh A, Lakshmanan G. In vitro antioxidant activity of achyranthes aspera linn. Int J Med Pharm. 2013;(3):67-78.
  23. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature, 1958;181(4617): 1199-1200. https://doi.org/10.1038/1811199a0
  24. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free radical biology and medicine. 1999;26(9-10):1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  25. Mihara M, Uciyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Analytical biochemistry. 1978;86(1):271-278. https://doi.org/10.1016/0003-2697(78)90342-1
  26. Lerebours E, Savoye G, Guedon C. [Epidemiology and natural history of chronic inflammatory bowel disease] Gastroenterol Clin Biol. 2003;27:S76-S80.
  27. Ishiguro K, Ando T, Maeda O, Hasegawa M, KadomatsuK, Ohmiya N, Niwa Y, Xavier R, Goto H. Paeonolattenuates TNBS-induced colitis by inhibiting NF-Band STAT1 transactivation. Toxicol Appl Pharmacol. 2006;217(1):35-42. https://doi.org/10.1016/j.taap.2006.07.002
  28. Itzkowitz SH, Yio X. Inflammation and cancer IV. Colorectal cancer in inflammatory bowel disease: the roleof inflammation. Am J Physiol Gastrointest Liver Physiol. 2004;287(1):G7-17.19. https://doi.org/10.1152/ajpgi.00079.2004
  29. Fazio V. Current status of surgery for inflammatory bowel disease. Digestion. 1998;59(5):470-480. https://doi.org/10.1159/000007524
  30. Yun BR, Weon JB, Lee B, Lee J, Eom MR, Choong JM. Quantitative analysis of Atractylenolides I and III in Atractylodes japonica. Kor. J. Pharmacogn. 2013;44(1):53-59.
  31. Park MH, Kim CJ, Lee JY, Keum CY, Kim IS, Jin CH, Ji JG, Kim SK. Anti-arthritic effect of radiation mutant perilla frutescensvar. crispa and Atractylodes macrophala koidz. Journal of the Korean Applied Science and Technology. 2020;37(1):102-113. https://doi.org/10.12925/JKOCS.2020.37.1.102
  32. Han J H, Kim JH, Kim SG, Jung SH, Kim DH, Kim GE, Whang WK. Anti-oxidative compounds from the aerial parts of Atractylodes macrocephala Koidzumi. Yakhak hoeji. 2007;(51)2:88-95.
  33. Lee SO, Seo JH, Lee JH, Yoo MY. Inhibitory effects of the rhizome extract of Atractylodes japonica on the proliferation of human tumor cell lines. Korean Journal of Pharmacognosy. 2005;(36)3:201-204.
  34. Choi SH. Pharmacological and Molecular studies of Atracylodes japonica Koidzumi on anti-oxidant activity. Kyung Hee University Graduate School. Seoul. 2011.
  35. Lee BB, Park SR, Han CS, Han DY, Park E, Park HR, Lee SC. Antioxidant activity and inhibition activity against α-amylase and α-glucosidase of Viola mandshurica extracts. J Korean Soc Food Sci Nutr 2008;37(4):405-409. https://doi.org/10.3746/JKFN.2008.37.4.405
  36. Heidari R, Jamshidzadeh A, Niknahad H, Mardani E, Ommati MM, Azarpira N, Khodaei F, Zarei A, Ayarzadeh M, Mousavi S, Abdoli N, Yeganeh BS, Saeedi A, Najibi A. Effect of taurine on chronic and acute liver injury: focus on blood and brain ammonia. Toxicol Rep. 2016;3:870-879. https://doi.org/10.1016/j.toxrep.2016.04.002
  37. Ji LL, Oxidative stress during exercise implication of antioxidant nutrients. Free Radical Biological. & Medicine. 1995;18(6):1079-1086. https://doi.org/10.1016/0891-5849(94)00212-3
  38. Kim JH. Protective effect of purple sweet potato hot water extract added to Bacillus subtilis- fermented soymilk against oxidative stress and cognition ability. Pusan National University Graduate School. Pusan. 2011.
  39. Anggard E. Nitric oxide: mediator, murderer, andmedicine. Lancet. 1994;9:1199-1206. https://doi.org/10.1016/S0140-6736(94)92405-8
  40. Yeom Y, Kim Y. The sasa quelpaertensis leaf extract inhibits the dextran sulfate sodium-induced mouse colitis through modulation of antioxidant enzyme expression. J. Cancer Prev. 2015;20:136-146. https://doi.org/10.15430/JCP.2015.20.2.136