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http://dx.doi.org/10.6116/kjh.2022.37.1.19.

Antioxidant Effect of Atractylodes macrocephala Koidzumi in DSS-induced Ulcerative Colitis Model  

Park, Seok Man (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Lee, Se Hui (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Jeong, Da un (Research Center for Herbal Convergence on Liver Disease, Daegu Haany University)
Cho, Su-Jung (Research Center for Herbal Convergence on Liver Disease, Daegu Haany University)
Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Park, Hae-Jin (DHU Bio Convergence Testing Center, Daegu Haany University)
Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.37, no.1, 2022 , pp. 19-29 More about this Journal
Abstract
Objectives : Although the pharmacological effects of anti-inflammatory and antioxidant action of Atractylodes macrocephala Koidzumi water extract (AM) have been proven from many studies, reports on the antioxidant effect of AM on ulcerative colitis (UC) are scarce. Therefore, we aimed at evaluating the anti-oxidant effect of AM on the DSS-induced UC model. Methods : To induce ulcerative colitis, 8-week-old male Balb/c mice received 5% DSS in drinking water for 1 week. After 1 week of adaptation, mice were divided into four groups (n=8 each) for use as normal (Normal), DSS Control (Control), DSS + AM 100 mg/kg (AM100)-treatment, DSS + AM 200 mg/kg (AM200)-treatment. After 1 week of the experiment, the animals were sacrificed, and the extracted colon tissue was analyzed for protein through western blot. Results : As a result of confirming the macroscopic changes in colon tissues to confirm the therapeutic effects of AM, the decrease in colon length was suppressed in the AM treatment group compared to the control group. In addition, as a result of biochemical analysis, AM administration significantly reduced serum glutamic oxalacetic transaminase, glutamic pyruvate transaminase levels and tissue malondialdehyde levels. As a result of confirming the protein expression level through western blot, AM administration significantly decreased the expression of NADPH-related proteins such as NOX2, p22phox, and iNOS, but significantly increased the expression of SOD, catalase, and GPx-1/2. Conclusions : AM may improve DSS-induced UC in mice by modulating NADPH and antioxidant-related proteins. In conclusion, AM showed an antioxidant effect through the improvement of oxidative stress on UC.
Keywords
oxidative stress; antioxidant; Atractylodes macrocephala Koidzumi; dextran sulfate sodium; ulcerative colitis;
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Times Cited By KSCI : 3  (Citation Analysis)
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