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http://dx.doi.org/10.5656/KSAE.2022.02.1.063

Improvement of Silkworm Egg Microinjection Using 3D Printing Technology  

Jeong, Chan Young (Department of Agricultural Biology, National Institute of Agricultural Science)
Lee, Chang Hoon (Department of Agricultural Biology, National Institute of Agricultural Science)
Seok, Young-Seek (Gangwon-do Agricultural Product Registered Seed Station)
Yong, Sang Yeop (Gangwon-do Agricultural Product Registered Seed Station)
Kim, Seong-Wan (Department of Agricultural Biology, National Institute of Agricultural Science)
Kim, Kee Young (Department of Agricultural Biology, National Institute of Agricultural Science)
Park, Jong Woo (Department of Agricultural Biology, National Institute of Agricultural Science)
Publication Information
Korean journal of applied entomology / v.61, no.1, 2022 , pp. 249-254 More about this Journal
Abstract
Silkworms, which have for long been used as an insect resource for industrialization, have recently attracted attention as potential bio-factories for the production of novel biomaterials. In this regard, material production is typically achieved based on transformation technology, mediated via microinjection, in which a target gene is inserted into eggs containing an embryo. However, an essential step in the microinjection procedure is egg fixation, which can be a time-consuming and laborious task. Therefore, in this study, using the 3DCADian program, we adopted a 3D printing approach to model egg liners and glue drawers, which can contribute to facilitating egg alignment and fixation, thereby enhancing transformation efficiency by reducing time consumption and fatigue. After rendering using Fusion 360, the two supplementary tools were produced by printing with nylon resin (PA12) and Sinterit Lisa Pro. Subsequent analysis of the time required to fix eggs on glass slides using the two manufactured tools, revealed that the processing time was reduced by approximately 18.6% when the two tools were used compared with when these tools were not used. These innovations not only reduced fatigue but also contributed to more effective use of the microscope and manipulator for microinjection. Consequently, we believe that with additional research and refinement, the egg liner and glue drawer developed in this study could be used to enhance silkworm transformation efficiency and study similar transformation systems in other industrial insects.
Keywords
Silkworm egg; Microinjection; Supplementary tool; 3D printing;
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1 Melgar-Lalanne, G., Hernandez-Alvarez, A.J., Salinas-Castro, A., 2019. Edible insects processing: traditional and innovative technologies. Compr. Rev. Food Sci. Food Saf. 18, 1166-1191.   DOI
2 Tamura, T., Kuwabara, N., Uchino, K., Kobayashi, I., Kanda, T., 2007. An improved DNA injection method for silkworm eggs drastically increases the efficiency of producing transgenic silkworms. J. Insect Biotechnol. Sericol. 76, 155-159.
3 Tamura, T., Thibert, C., Royer, C., Kanda, T., Abragam, E., Kamba, M., Komoto, N., Thomas, J.L., Mauchamp, B., Chavancy, G., Shirk, P., Fraser, M., Prudhomme, J.C., Couble, P., 2000. Germline transformation of the silkworm Bombyx mori L. using a piggyback transposon-derived vector. Nat. Biotechnol. 18, 81-84.   DOI
4 Coates, C.J., Jasinskiene, N., Miyashiro, L., James, A.A., 1998. Mariner transposition and transformation of the yellow fever mosquito, Aedes aegypti. Proc. Natl. Acad. Sci. U. S. A. 95, 3748-3751.   DOI
5 Kang, P.D., Lee, S.U., Jung, I.Y., Shon, B.H., Kim, Y.S., Kim, K.Y., Kim, M.J., Hong, I.P., Lee, K.G., Park, K.Y., 2007. Breeding of new silkworm variety golden silk, a yellow cocoon color for spring rearing season. Korean J. Seric. Sci. 49, 14-17.
6 Kumari, S.S., Subbarao, S.V., Misra, S., Murty, U.S., 2011. Screening strains of the mulberry silkworm, Bombyx mori, for thermotolerance. J. Insect Sci. 11, 1-14.
7 Okano, F., Satoh, M., Ido, T., Okamoto, N., Yamada, K., 2000. Production of canine IFN-gamma in silkworm by recombinant baculovirus and characterization of the product. J. Interferon Cytokine Res. 20, 1015-1022.   DOI
8 Rubin, G.M., Spradling, A.C., 1982. Genetic transformation of Drosophila with transposable element vectors. Science 218, 348-353.   DOI
9 Kim, T.K., Yong, H.I., Kim, Y.B., Kim, H.W., Choi, Y.S., 2019. Edible insects as a protein source: a review of public perception, processing technology, and research trends. Food Sci. Anim. Resour. 39, 521-540.   DOI
10 Ueda, Y., Sakurai, T., Yanai, A., 1993. Homogeneous production of feline interferon in silkworm by replacing single amino acid code in signal peptide region in recombinant baculovirus and characterization of the product. J. Vet. Med. Sci. 55, 251-258.   DOI