• Applied Microscopy
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• v.37 no.4
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• pp.209-217
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• 2007

The system of wound healing is very complex biological processing that includes inflammatory, reepithelialization, and matrix construction. For identification of the transitional pathway of the keratinocytes, we have employed immunohistochemical analysis using cytokeratin antibody after wounding. Epithelium in skin of the frog(Bombina orientalis) was examined with transmission electron microscopy. Cytokeratin was expressed in normal basal and gland cavity cells. At 3-hour basal layer cells were strong positive, however cells of the upper layer were negative reaction. Day1 and 2 after post-wounding, regenerating epithelial cell layer was positive reaction, especially basal layer cells were strong positive. At day 10 after wounding, the degree of positive reaction to basal cells of regenerating epithelial tissue was equal to day 7 wound tissue. At day of 19th, basal and spinous layer cells were strong positive reaction. Regenerating epithelial cells were positive but some basal cells were strong positive at day 27. From this result, we identified that the migration of the keratinocytes in amphibian skin wounds is initiated from basal layer fells and the keratinocytes migrate into basal and middle of the wound area.

• Biomedical Science Letters
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• v.10 no.4
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• pp.361-366
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• 2004

The purpose of this study was to determine the effect of high voltage pulsed current (HVPC) stimulation on proliferative activities of basal keratinocytes by measured nucleolar organizer region (NOR) expression and thickness of spinous layer in aged rat skin. Fifty-one weeks old twelve male Sprague-Dawley rats (300∼350g) were divided into control and HVPC stimulation groups. Each animal's hair on the back were removed. The HVPC stimulation group received an negative monophasic twin peak pulsed current stimulation with 50 V, while the control group was given the same treatment without electricity. The rats were sacrificed after 3 weeks. The biopsy specimens were fixed in formalin, embedded in paraffin and stained with hematoxyline-eosin and silver nitrate. The thickness of basal to granular layer of the epidennis were measured using a light microscope and computerized image analysis system. The number of argyrophilic nucleolar organizer region (AgNOR) were counted using a light microscope and computerized image analysis system and calculated as the mean number of AgNOR per nucleus in the basal keratinocyte. By using a Student's t-test, an increase in the thickness of basal-spinous layer (P<0.001) of epidermis can be observed in HVPC stimulation rats as compared with the control rats, whereas the thickness of the granular layer is not affected. A Student's t-test showed a significantly higher mean NOR number per nucleus of the basal keratinocyte in the HVPC stimulation rats than control rats (P<0.001). There was significantly positive correlation between the NOR number and the thickness of basal-spinous layer (r=0.80, P<0.05). These results suggest that the HVPC stimulation may increase the thickness of spinous layer in the epidennis due to increased proliferative activities of basal keratinocytes in epidennis in aged rat skin.

• Journal of the Korean Society of Fashion and Beauty
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• v.3 no.2 s.2
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• pp.12-17
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• 2005

Efficiency study of chamomile and Thyme essential oil about the basal layer & sebaceous gland in dry-skin induced by surfactant is as following : 1. Formal observation of outer skin layer and basal layer There was refreshing effects about Surfactant induction skin inflammation in Chamomile, Thyme essential oil processing group but effect in surface layer appeared by higher thing in Chamomile essential oil and it could know that thing which do not use Thyme essential oil in case Thyme essential oil is ultraviolet rays allergy at skin care because Melanocyte appear on the surface is bigger than Chamomile essential oil that it was usefully reaction in basal layer. 2. Formal observation result of sebaceous glands. There are few damages in all group but there is recuperative through excessive water damage by excessive a sebaceous gland made of mulberry bark secretion because Thyme essential oil application group cause chapped skin phenomenon after surface-active agent processing but is seen that difference does not exist greatly and Thyme essential oil that general skin is strong in permeation is effective, but Surfactant induction skin inflammation estimates that Chamomile essential oil is effective. Therefore, it was considered that use of Chamomile essential oil is effectiveness than Thyme essential oil by essential oil used for recovery after damage of inflammation etc..

• Applied Microscopy
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• v.20 no.2
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• pp.71-80
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• 1990

It was investigated the morphological changes of differentiating epidermal cells in chick embryos. Ectodermal cells at 3 days after incubation were cuboidal, their nuclei were large, and rough endoplasmic reticulum and mitochondria were distributed in the cytoplasm. At 5 days after incubation, there were periderm and one basal layer in epidermis. The cells of basal layer were columnar, their nuclei were round, and rough endoplasmic reticulum and free ribosomes were developed. Also, peridermal cells were flat, chromatins were partially condensed and glycogen particles were abundant. No periderm showed and cells of basal layer formed intermediate layer at 9 days after incubation. Basal cells of intermediate layer were cuboidal, neighboring cells were anchored by desmosomes and tonofibrils and free ribosomes were evenly scattered. At 15 days after incubation, stratum corneum and stratum germinativum were distinguished. In cells of stratum germinativum, tonofibrils, free ribosomes and desmosomes were well developed. And then, the shedding of stratum corneum were showed at 17 days after incubation and stratum corneum were well developed after hatching.

• Journal of Life Science
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• v.24 no.5
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• pp.532-542
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• 2014

This study describe seasonal patterns in the variation of phytoplankton frequency in the water surface and basal layers and their spatial distributions at seven stations in Hwadang-ri, Goseng-gun in 2013. The phytoplankton community at Hwadang-ri was very diverse, with 60 taxa identified, representing three classes. Diatoms (Bacillariophyceae) exhibited the greatest diversity, with 41 taxa identified. These were followed by the dinoflagellates Dinophyceae, Cryptophyceae, and Eugenophyceae, with 16 taxa, two taxa, and one taxon, respectively. Water surfaces were shown with the relative individual density or abundance across areas. Except in January, Shannon-Weaver indices of diversity of the water surface layer were lower than those of the basal layer. In addition, evenness indices of the basal layer were higher than those of the water surface layer, except in January. For the community as a whole, the values of ${\beta}$-diversity were low for the seven stations: 1.125 for the water surface layer and 1.481 for the basal layer. Seasonal values for ${\beta}$-diversity were similar at the seven stations: 1.725 for the water surface layer and 1.347 for the basal layer. The phytoplankton community showed high taxonomic homogeneity in all four seasons, in addition to similar trends in seasonal development at depths in the same stations. However, the size distribution of the abundance and biomass showed a statistically significant west-east difference.

• Korean Journal of Ichthyology
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• v.19 no.2
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• pp.120-127
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• 2007

To investigate the skin of the flat-headed goby, Luciogobius guttatus, it was used 8 body regions such as the head, the upper and lower jaw, the chin, the back, lateral region, abdomen, and the operculum. The epidermis consisted of three layers: the outermost layer, middle layer and stratum germinativum (basal layer). The outermost layer consisted of rather flattened cells arranged in 1 to 4 layers and mucous cells. The middle layer consisted of large epidermal cells occupying the height of the epidermis with 1 to 10 layers, causing a web-shaped structure. Due to the large epidermal cells of the middle layer, L. guttaus had a thick epidermis. The large epidermal cell contains tonofilaments, lucent contents, and desmosome. The basal layer was comprised of a single layer having cuboidal cells. A large number of fine blood capillaries were found just under the basal layer. The dermis consisted mostly of stratum compactum with numerous blood capillaries but had no scale.

• Geomechanics and Engineering
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• v.16 no.3
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• pp.233-245
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• 2018

Interest to basal reinforced piled embankments is increasing recently due to their rapid construction and reliability. A comprehensive parametric study is conducted to determine effects of pile properties, reinforcement stiffness, embankment properties and soft soil properties into settlements, pressures and excess pore water pressure development and dissipations. Results which are obtained by using one-layer reinforcement during construction are compared with the results obtained by using two-layer reinforcement during construction. Finite element method is used during the parametric study. Second layer of reinforcement is placed in five different positions in order to reveal effects of reinforcement position into behaviour. Traffic load is also taken into consideration during the study. Differences between the results without presence of traffic loading and with presence of traffic loading is stated in this the study.

• Applied Microscopy
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• v.1 no.1
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• pp.11-17
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• 1969

Conidio spores of Aspergillus niger (strain No. NRRL 330) cultured on potato dextrose agar media were studied by electron microscopy, using the thin sectioning techniques. Conidio spores to be sectioned were fixed by triple methods with $K_2Cr_2O_7$, Glutaraldehyde and $OsO_4$. After dehydrated with alcohol, the specimens were embedded in metacrylate and epon resin media, and thinly sectioned by Porter-Blum MT-2. After sectioned these specimens were negative-stained with uranyl acetate and observed. by Hitachi HS-6 electron microscope. The results of this experiment were summarized as follows. 1. The structures of spore ,wall system seem to be formed 4 layers; exosporium, basal layer, spore coat and unit cell membrane. The protuberance of spore surface that was looked like hair appears to be protrusived from the basal layer. 2. The 3 layers of unit cell membrane was constituted outer layer membrane, inner layer membrane and inter-mediate light layer. 3. The structures of intra cytoplasmic membrane appear as spiral form which was consisted of 3 layers membrane system; outer membrane, inner membrane, and intermediate layer, which has pits. 4. The cement substance of spore coat and cortex may be changed quantitatively by physiological state in cell. 5. In some cases, we observed that the ribosome was transformed into poly ribosome group, and the storage materials and the protein crystals were changed variously. It. has been suggested that the morphological change of some cytoplasmic materials may be caused by some specialized function of the physiological stage.

• Korean Journal of Veterinary Research
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• v.34 no.2
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• pp.237-242
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• 1994

The present study was focussed to assess the proliferating cells in the distal epiphyseal tissue of the chicken femur by immunohistochemical staining methods. Four chickens were administrated intraperitoneally by twice consecutive injections, 1 day interval with bromodeoxyuridine(Brdur, 0.05 mg/gm BW/time), and then were killed by exsanguination of jugular vein at 2 hours after last injection. Samples were taken from femur distal epiphyseas of chicken. Labeling indexes(LI) were calculated as the ratio of the number of anti-Brdur monoclonal antibody-labeled cells in the each tissue layers from basal layer of the integument to bone marrow. The overall LI were found to be $13.90{\pm}3.44%$, $30.03{\pm}7.52%$, $16.00{\pm}9.41%$, $0.00{\pm}0.00%$ and $60.03{\pm}13.39%$ at basal layer of integument, perichordrium, reseving zone in cartilage, hypertrophic zone in cartilage and bone marrow respectively. LI in proliferating zone of cartilage were found to be $36.99{\pm}7.59%$, $32.83{\pm}5.38%$ and $22.02{\pm}6.27%$ at reserving zone side region, middle region, and hypertrophic zone side region respectively. The tissue layers with higher LI were odered as bone marrow, reserving zone side region in proliferating zone, middle region in proliferating zone, perichondrium, hypertrophic zone side region in proliferating zone. reserving zone of cartilage and basal layer of integument. These data indicate that the overall LI in the each tissue layer of distal epiphyseas of the chicken femur were concluded to be higher than that in another tissue of adult birds but hypertrophic zone of cartlage were appeared to be not proliferating cells.

• Korean Journal of Bronchoesophagology
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• v.1 no.1
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• pp.64-68
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• 1995

Vocal folds injury from vocal abuse is important topics of phonosurgery. Recent advances in diagnostic equipment, phonosurgery and speech analysis equipment have provided a lot of Information about fine movement of the vocal folds. However, predicting the reaction of the vocal folds to phonatory trauma remains difficult. The vocal folds need to withstand great vibratory and shearing stress and anchoring fibers of basement membrane Bone play a role in maintaining structural integrity of histologically different epidermis and superficial layer of lamina propria(cover of vocal folds). The purpose of this study is to demonstrate the changes of anchoring fibers in vocal polyp using transmission electron microscope. Various defects were observed : a irregular thickening of basement membrane, a near absence of normal anchoring fiber, a lot of electron dense material in superficial layer of lamina propria, a destruction of hemidesmosome and many vesicles carrying electron dense material In basal keratinocyte. These observations were suggestive of a hyperactivity of basal keratinocyte of vocal folds epithelium in response to vibratory stress.