Korean Journal of Veterinary Research (대한수의학회지)
- Volume 34 Issue 2
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- Pages.237-242
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- 1994
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Immunohistochemical observations of proliferating cells in distal epiphyseal tissue of chicken femurs
닭의 대퇴부 골단조직의 세포증식에 대한 면역조직화학적 관찰
- Kwak, Soo-dong (College of Veterinary Medicine, Gyeongsang National University) ;
- Kim, Chong-sup (College of Veterinary Medicine, Gyeongsang National University) ;
- Kang, Chung-boo (College of Veterinary Medicine, Gyeongsang National University)
- Received : 1994.03.18
- Published : 1994.04.30
Abstract
The present study was focussed to assess the proliferating cells in the distal epiphyseal tissue of the chicken femur by immunohistochemical staining methods. Four chickens were administrated intraperitoneally by twice consecutive injections, 1 day interval with bromodeoxyuridine(Brdur, 0.05 mg/gm BW/time), and then were killed by exsanguination of jugular vein at 2 hours after last injection. Samples were taken from femur distal epiphyseas of chicken. Labeling indexes(LI) were calculated as the ratio of the number of anti-Brdur monoclonal antibody-labeled cells in the each tissue layers from basal layer of the integument to bone marrow. The overall LI were found to be