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The Temporal and Spatial Expression of the Cytokeratin in Keratinocytes during Cutaneous Wound Healing on the Amphibian(Bombina orientalis)  

Lim, Do-Seun (Department of Dental Hygiene, College of Health Science, Eulji University)
Jeong, Soon-Jeong (Department of Oral Histology, College of Dentistry, Chosun University)
Moon, Myung-Jin (Department of Biological Science & Basic Science, Dankook University)
Jeong, Moon-Jin (Department of Oral Histology, College of Dentistry, Chosun University)
Publication Information
Applied Microscopy / v.37, no.4, 2007 , pp. 209-217 More about this Journal
Abstract
The system of wound healing is very complex biological processing that includes inflammatory, reepithelialization, and matrix construction. For identification of the transitional pathway of the keratinocytes, we have employed immunohistochemical analysis using cytokeratin antibody after wounding. Epithelium in skin of the frog(Bombina orientalis) was examined with transmission electron microscopy. Cytokeratin was expressed in normal basal and gland cavity cells. At 3-hour basal layer cells were strong positive, however cells of the upper layer were negative reaction. Day1 and 2 after post-wounding, regenerating epithelial cell layer was positive reaction, especially basal layer cells were strong positive. At day 10 after wounding, the degree of positive reaction to basal cells of regenerating epithelial tissue was equal to day 7 wound tissue. At day of 19th, basal and spinous layer cells were strong positive reaction. Regenerating epithelial cells were positive but some basal cells were strong positive at day 27. From this result, we identified that the migration of the keratinocytes in amphibian skin wounds is initiated from basal layer fells and the keratinocytes migrate into basal and middle of the wound area.
Keywords
Amphibian; Bombina orientalis; Cutaneous; Cytokeratin; Keratinocyte; Wound healing;
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