Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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The Temporal and Spatial Expression of the Cytokeratin in Keratinocytes during Cutaneous Wound Healing on the Amphibian(Bombina orientalis)

양서류 피부 상처회복과정 중 각질화세포 cytokeratin의 분포

  • Lim, Do-Seun (Department of Dental Hygiene, College of Health Science, Eulji University) ;
  • Jeong, Soon-Jeong (Department of Oral Histology, College of Dentistry, Chosun University) ;
  • Moon, Myung-Jin (Department of Biological Science & Basic Science, Dankook University) ;
  • Jeong, Moon-Jin (Department of Oral Histology, College of Dentistry, Chosun University)
  • 임도선 (을지대학교 보건과학대학 치위생학과) ;
  • 정순정 (조선대학교 치과대학 구강조직학교실) ;
  • 문명진 (단국대학교 첨단과학대학 생물학과) ;
  • 정문진 (조선대학교 치과대학 구강조직학교실)
  • Published : 2007.12.31
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Abstract

The system of wound healing is very complex biological processing that includes inflammatory, reepithelialization, and matrix construction. For identification of the transitional pathway of the keratinocytes, we have employed immunohistochemical analysis using cytokeratin antibody after wounding. Epithelium in skin of the frog(Bombina orientalis) was examined with transmission electron microscopy. Cytokeratin was expressed in normal basal and gland cavity cells. At 3-hour basal layer cells were strong positive, however cells of the upper layer were negative reaction. Day1 and 2 after post-wounding, regenerating epithelial cell layer was positive reaction, especially basal layer cells were strong positive. At day 10 after wounding, the degree of positive reaction to basal cells of regenerating epithelial tissue was equal to day 7 wound tissue. At day of 19th, basal and spinous layer cells were strong positive reaction. Regenerating epithelial cells were positive but some basal cells were strong positive at day 27. From this result, we identified that the migration of the keratinocytes in amphibian skin wounds is initiated from basal layer fells and the keratinocytes migrate into basal and middle of the wound area.

상처회복은 염증, 재상피화 그리고 기질의 재형성등이 관여하는 복잡한 과정이다. 이중 재상피화에 관여하는 각질화세포의 이동경로를 분석하기 위하여 무당개구리 피부 상처유도 후 투과전자현미경과 cytokeratin에 대한 조직면역화학법을 이용하였다. 정상조직의 cytokeratin발현은 기저층의 세포들과 선상피에서 확인되었다. 상처 유도후 3시간 조직에서, 기저세포층에서 강한 반응이 관찰되었고, 1일과 2일 사이에서는 재생되는 각질화세포에서 강한 면역반응이 확인되었다. 상처반응 중기인 7일부터 10일 사이에서도 재생된 세포의 기저층세포에서 강한 반응이 일어났다. 19일경과의 조직에서는 기저층과 유극층의 세포들에서 cytokeratin의 발현이 증가하였다. 따라서, 재상피화에 관여하는 각질화세포는 기저층의 세포로부터 시작하여 상처부위로 이동하여 과립층과 유극층으로 분화됨으로서 재상피가 진행되었다.

Keywords

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