Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Structure and Histochemistry of the Skin of a Flat-headed Goby, Luciogobius guttatus (Gobiidae, Pisces) from Korea

한국산 미끈망둑 Luciogobius guttatus (망둑어과) 피부의 구조 및 조직화학적 특성

  • Park, Jong-Young (Faculty of Biological Sciences and Institute for Biodiversity Research, Chonbuk National University) ;
  • Lee, Yong-Joo (Jeonju National University of Education) ;
  • Kim, Ik-Soo (Faculty of Biological Sciences and Institute for Biodiversity Research, Chonbuk National University)
  • 박종영 (전북대학교 생물과학부.생물다양성연구소) ;
  • 이용주 (전주교육대학교 과학교육학과) ;
  • 김익수 (전북대학교 생물과학부.생물다양성연구소)
  • Received : 2007.05.14
  • Accepted : 2007.06.04
  • Published : 2007.06.30
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Abstract

To investigate the skin of the flat-headed goby, Luciogobius guttatus, it was used 8 body regions such as the head, the upper and lower jaw, the chin, the back, lateral region, abdomen, and the operculum. The epidermis consisted of three layers: the outermost layer, middle layer and stratum germinativum (basal layer). The outermost layer consisted of rather flattened cells arranged in 1 to 4 layers and mucous cells. The middle layer consisted of large epidermal cells occupying the height of the epidermis with 1 to 10 layers, causing a web-shaped structure. Due to the large epidermal cells of the middle layer, L. guttaus had a thick epidermis. The large epidermal cell contains tonofilaments, lucent contents, and desmosome. The basal layer was comprised of a single layer having cuboidal cells. A large number of fine blood capillaries were found just under the basal layer. The dermis consisted mostly of stratum compactum with numerous blood capillaries but had no scale.

한국산 미끈망둑(Luciogobius guttatus)의 피부구조가 머리, 위턱, 아래턱, 뺨, 등, 체측, 배, 아가미뚜껑 등 모두 8개 부분에서 비교 조사되었다. 이들 모두 표피는 맨바깥층, 중간층, 기저층으로 구성되었다. 맨바깥층은 1~4층의 편평세포와 점액세포로 구성되었으며, 중간층은 1~10층의 대형 표피세포를 가지며 이러한 표피세포는 마치 거미줄 같은 형태를 보여주고 있다. 이러한 대형표피세포의 존재는 두꺼운 표피를 갖도록 하며, tonofiilament와 desmosome을 가진다. 한편 기저층은 한 층의 입방상피로 구성되어 있으며, 많은 모세혈관들이 기저층 바로 아래부분에 존재한다. 진피에는 비늘이 존재하지 않으며 거의 대부분 모세혈관을 가지는 치밀층으로 구성되어 있다.

Keywords

Acknowledgement

Supported by : Jeonju Nationational University of Education

References

  1. Al-Kadhomiy, N.K. and G.M. Hughes. 1988. Histological study of different regions of the skin and gills in the mudskipper, Boleophthalmus boddarti with respect to their respiratory function. J. Mar. Biol. Ass. U.K., 68 : 413-422 https://doi.org/10.1017/S0025315400043319
  2. Bridges, C.R. 1988. Respiratory adaptations in intertidal fish. Amer. Zool., 28 : 79-96 https://doi.org/10.1093/icb/28.1.79
  3. Graham, J.B. 1997. Air breathing fishes: Evolution, diversity, and adaptation. Academic Press, San Diego, 299pp
  4. Gurr, G.T. 1956. A practical manual of medical and biological staining techniques. Interscience, New York
  5. Horn, M.H., K.L.M. Martin and M.A. Chotkowski. 1999. Intertidal fishes. Academic Press, San Diego, 399pp
  6. Horn, M.H. and K.C. Riegle. 1981. Evaporative water loss and intertidal vertical distribution in relation to body size and morphology of stichaeoid fishes California. J. Exp. Mar. Biol. Ecol., 50 : 273-288 https://doi.org/10.1016/0022-0981(81)90054-X
  7. Jakubowski, M. 1958. The structure and vascularization of the skin of the pond-loach (Misgurnus fossilis L.). Acta Biol. Cracoviensia, 1 : 113-127
  8. Johansen, K. 1970. Air breathing in fishes. In: Hoar W.S. and D.J. Randall (eds), Fish Physiology IV. Academic Press, New York, pp. 361-411
  9. Lev, R. and S.S. Spicer. 1964. Specific staining of sulphated groups with alcian blue at low pH. J. Histochem. Cytochem., 12 : 309 https://doi.org/10.1177/12.4.309
  10. Liem, K.F. 1967. Functional morphology of the integumentary, respiratory, and digestive systems of the synbranchoid fish, Monopterus albus. Copeia, (1967) : 375-388
  11. Martin, K.L.M. 1995. Time and tide wait for no fish: Intertidal fishes out of water. Environ. Biol. Fish., 44 : 165-181 https://doi.org/10.1007/BF00005914
  12. Mittal, A.K. and J.S.D. Munshi. 1971. A comparative study of the structure of the skin of certain air-breathing fresh -water teleosts. J. Zool. Lond., 163 : 515-532 https://doi.org/10.1111/j.1469-7998.1971.tb04547.x
  13. Mittal, A.K. and T.K. Banerjee. 1974. Structure and keratinization of the skin of a fresh-water teleost Notopterus notopterus (Notopteridae, Pisces). J. Zool. Lond., 174 : 314-355
  14. Mittal, A.K., M.A. Whitear and S.K. Agarwal. 1980. Fine structure and histochemistry of the epidermis of the fish, Monopterus cuchia. J. Zool. Lond., 191 : 107-125 https://doi.org/10.1111/j.1469-7998.1980.tb01452.x
  15. Nelson, J.S. 1994. Fishes of the World. 3rd ed. John Wiley and Sons, New York, 600pp
  16. Park, J.Y. 2002a. Morphology and histochemistry of the skin of the spined cobitid fish, Iksookimia koreensis, in relation to respiration. Folia Zool., 51 : 241-247
  17. Park, J.Y. 2002b. Structure of the skin of an air-breathing mudskipper fish, Periophthalmus magnuspinnatus. J. Fish Biol., 60 : 1543-1550 https://doi.org/10.1111/j.1095-8649.2002.tb02446.x
  18. Park, J.Y. and I.S. Kim. 1999. Structure and histochemistry of skin of mud loach, Misgurnus anguillicaudatus (Pisces, Cobitidae), from Korea. Korean J. Ichthyol., 11 : 109-116
  19. Park, J.Y. and I.S. Kim. 2000. Structure and cytochemistry of skin in spined loach, Iksookimia longicorpus (Pisces, Cobitidae). Korean J. Ichthyol., 12 : 25-32
  20. Park, J.Y., I.S. Kim and S.Y. Kim. 2000. Histological study on skin of the amphibious fish, Periophthalmus modestus. Korean J. Biol. Sci., 4 : 315-318 https://doi.org/10.1080/12265071.2000.9647562
  21. Park, J.Y., Y.I. Lee, I.S. Kim and S.Y. Kim. 2003a. A comparative study of the regional epidermis of an amphibious mudskipper fish, Boleophthalmus pectinirostris (Gobiidae, Pisces). Folia Zool., 52 : 431-440
  22. Park, J.Y., I.S. Kim and S.Y. Kim. 2003b. Structure and histochemistry of the skin of a torrent catfish, Liobagrus mediadiposalis. Environ. Biol. Fish., 66 : 3-8 https://doi.org/10.1023/A:1023298520696
  23. Park, J.Y., I.S. Kim and Y.J. Lee. 2006. A Study on the vascularization and structure of the epidermis of the air-breathing mudskipper, Periophthalmus magnuspinnatus (Gobiidae, Teleostei), along different parts of the body. J. Applied Ichthyol., 22 : 62-67 https://doi.org/10.1111/j.1439-0426.2006.00696.x
  24. Spicer, S.S. 1965. Histochemistry manual. The university of Kansas Medical Center, Kansas City, 54pp
  25. Steedman, H.F. 1950. Alcian blue 8G: a new stain for mucin. Quart. J. Micr. Sci., 1 : 477-479
  26. Suzuki, N. 1992. Fine structure of the epidermis of the mudskipper, Periophthalmus modestus (Gobiidae). Japan. J. Ichthyol., 8 : 379-396
  27. Tock, E.P.C. and A.G.E. Pearse. 1965. Preservation of tissue mucins by freeze-drying and vapour fixation. J. R. microsc. Soc., 84 : 519-537 https://doi.org/10.1111/j.1365-2818.1965.tb02153.x
  28. Whitear, M. 1986. The skin of fishes including cyclostomes: epidermis. In: Bereiter-Hahn, J., A.G. Matoltsy and K.S. Richards (eds), Biology of the integument Vol. 2 Vertebrates. Springer Verlag, New York, pp. 9-64
  29. Yokoya, S. and O.S. Tamura. 1992. Fine structure of the skin of the amphibious fishes, Boleophthalmus pectinirostris and Periophthalmus cantonensis, with special reference to the location of blood vessels. J. Morphol., 214 : 287-297 https://doi.org/10.1002/jmor.1052140305
  30. Zhang, J., T. Taniguchi., T. Takita and A.B. Ali. 2000. On the epidermal structure of Boleophthalmus and Scartelaos mudskippers with reference to their adaptation to terrestrial life. Ichthyol. Res., 47 : 359-366 https://doi.org/10.1007/BF02674263