• 대한수의학회지
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• 제50권2호
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• pp.125-131
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• 2010

Bacterial contamination is an unavoidable finding of the semen collection process in boar and can lead in deleterious effects on semen quality and longevity if left uncontrolled. The purpose of this study is to identify the bacteria in extended boar semen and to select the effective antimicrobials to control of the contaminants. Of 116 extended boar semen samples submitted from eight AI centers in Korea, 39 (33.6%) samples were positive for bacterial contamination. Among 39 contaminated semen, most of them (84.6%) were contaminated with one or two bacterial species and there was no significant difference between two age groups $(\leq\;24\;and\;>\;24\;month\;old).$ Stenotrophomonas maltophilia (n = 18) was the most predominant bacterium followed by Elizabethkingia meningoseptica (n = 12), Sphingomonas paucimobilis (n = 12), Myroides spp. (n = 5), Ochrobactrum anthropi (n = 3), and so on. Enrofloxacin (72.9%), florfenicol (72.9%), bacitracin (49.2%) and tylosin (49.2%) showed higher sensitivity compared with penicillin (13.6%) or aminoglycosides (6.8%-18.6%). Brucella spp., Leptospira spp., Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, Mycobacterium tuberculosis complex were not detected in semen by PCR.

• 대한약침학회지
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• 제10권2호통권23호
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• pp.87-91
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• 2007

Objectives : This experimental study was performed to investigate the continuous anti-bacterial potency of Jinpi-san on cultivation of Staphylococcus species(S. aureus, S. epidermidis) that induce eye disease. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to 50${\mu}$l diluted Jinpi-san(100%, 10%, 1%, 0.1%) on S. aureus, S. epidermidis that were cultivated from 2 to 6 days. Anti-bacterial potency was measured by the size of inhibition zone with change of volume(20${\mu}$l, 30${\mu}$l, 40${\mu}$l, 50${\mu}$l). Results : 1. Anti-bacterial potency of Jinpi-san on S. aureus was appeared in 100% and increased on 6 days. Anti-bacterial potency with change of volume was increased in propotion to increase volume. Anti-bacterial potency of Jinpi-san on S. aureus was appeared continuous. 2. Anti-bacterial potency of Jinpi-san on S. epidermidis was appeared in 100%, 10%, 1% on 2 days and in 100%, 10% on 6 days. In 100% Jinpi-san, Anti-bacterial potency of 6 days was increased, in 10%, Anti-bacterial potency of 2 days was increased, in 1%, Anti-bacterial potency of 6 days was disappear. Anti-bacterial potency with change of volume was increased in propotion to increase volume except for 20${\mu}$l of 6days and increased on 6 days, but bacteria was increased. Anti-bacterial potency of Jinpi-san on S. epidermidis wasn't appeared continuous. Conclusions : Anti-bacterial potency of Jinpi-san on cultivation of S. aureus was showed continuous, but on cultivation of S. epidermidis was not showed continuous.

• 식물병연구
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• 제6권1호
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• pp.6-9
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• 2000

1998년 수원, 성남, 양재, 대전의 비닐하우스에서 재배되고 있는 인삼고무나무(Ficus retusa), 황금대만고무나무(Ficus retusa \`Golden leaf\`), 벤자민고무나무(Ficus benjamina)에 새로운 세균병이 관찰되었다. 인삼고무나무의 뿌리에 무름증상을 보이는 세균병을 \"P.cichorii\"에 의한 인삼고무나무의 세균성뿌리썩음병\", 황금대만고무나무와 벤자민고무나무에 마름증상을 보이는 세균병을 \"P.viridiflava에 의한 황금대만고무나무와 벤자민고무나무의 세균성잎마름병\"으로 명명할 것을 제안한다.마름병\"으로 명명할 것을 제안한다.제안한다.

• The Plant Pathology Journal
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• 제29권4호
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• pp.386-396
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• 2013

Reactive oxygen species (ROS) generation in tomato plants by Ralstonia solanacearum infection and the role of hydrogen peroxide ($H_2O_2$) and nitric oxide in tomato bacterial wilt control were demonstrated. During disease development of tomato bacterial wilt, accumulation of superoxide anion ($O_2{^-}$) and $H_2O_2$ was observed and lipid peroxidation also occurred in the tomato leaf tissues. High doses of $H_2O_2$ and sodium nitroprusside (SNP) nitric oxide donor showed phytotoxicity to detached tomato leaves 1 day after petiole feeding showing reduced fresh weight. Both $H_2O_2$ and SNP have in vitro antibacterial activities against R. solanacearum in a dose-dependent manner, as well as plant protection in detached tomato leaves against bacterial wilt by $10^6$ and $10^7$ cfu/ml of R. solanacearum. $H_2O_2$- and SNP-mediated protection was also evaluated in pots using soil-drench treatment with the bacterial inoculation, and relative 'area under the disease progressive curve (AUDPC)' was calculated to compare disease protection by $H_2O_2$ and/or SNP with untreated control. Neither $H_2O_2$ nor SNP protect the tomato seedlings from the bacterial wilt, but $H_2O_2$ + SNP mixture significantly decreased disease severity with reduced relative AUDPC. These results suggest that $H_2O_2$ and SNP could be used together to control bacterial wilt in tomato plants as bactericidal agents.

• 대한약침학회지
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• 제10권2호통권23호
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• pp.19-23
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• 2007

Objectives : This experimental study was performed to investigate the continuous anti-bacterial potency of Sean-tang on cultivation of Staphylococcus species(S. aureus, S. epidermidis) that induce eye disease. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to 50${\mu}$l diluted Sean-tang(100%, 10%,1%, 0.1%) on S. aureus, S. epidermidis that were cultivated from 2 to 6 days. 1. Anti-bacterial potency was measured by the size of inhibition zone with change of volume(20${\mu}$l, 30${\mu}$l, 40${\mu}$l, 50${\mu}$l). Results : 1. Anti-bacterial potency of Sean-tang on S. aureus was appeared in 100% and increased on 6 days. Anti-bacterial potency with change of volume was increased in propotion to increase volume. Anti-bacterial potency of Sean-tang on S. aureus was appeared continuous. 2. Anti-bacterial potency of S. epidermidis was appeared in 100%, 10%, 1% on 2 days and in100%, 10% on 6 days. In 100% Sean-tang, Anti-bacterial potency of 6 days was increased, in 10%, 1%, Anti-bacterial potency of 2 days was increased. Anti-bacterial potency with change of volume was increased inpropotion to increase volume and increased on 6 days, but bacteria was increased. Anti-bacterial potency Sean-tang on S. epidermidis wasn't appeared continuous. Conclusions : Anti-bacterial potency of Sean-tang on cultivation of S. aureus was showed continuous, but on cultivation of S. epidermidis was not showed continuous.

• Clinical and Experimental Pediatrics
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• 제60권4호
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• pp.112-117
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• 2017

Purpose: The aims of this study were to compare serum procalcitonin (PCT) levels between febrile children with Kawasaki disease (KD) and those with bacterial or viral infections, and assess the clinical usefulness of PCT level in predicting KD. Methods: Serum PCT levels were examined in febrile pediatric patients admitted between August 2013 and August 2014. The patients were divided into 3 groups as follows: 49 with KD, 111 with viral infections, and 24 with bacterial infections. Results: The mean PCT level in the KD group was significantly lower than that in the bacterial infection group ($0.82{\pm}1.73ng/mL$ vs. $3.11{\pm}6.10ng/mL$, P=0.002) and insignificantly different from that in the viral infection group ($0.23{\pm}0.34ng/mL$, P=0.457). The mean erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) level in the KD group were significantly higher than those in the viral and bacterial infection groups (P<0.001 and P<0.001 for ESR, P<0.001 and P=0.005 for CRP, respectively). The proportion of patients in the KD group with PCT levels of >1.0 ng/mL was significantly higher in the nonresponders to the initial intravenous immunoglobulin treatment than in the responders (36% vs. 8%, P=0.01). Conclusion: PCT levels may help to differentiate KD from bacterial infections. A combination of disease markers, including ESR, CRP, and PCT, may be useful for differentiating between KD and viral/bacterial infections.

• Journal of Applied Biological Chemistry
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• 제44권4호
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• pp.173-176
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• 2001

This report concerns the role of indole-3-acetic acid (IAA) in bacterial pustule disease of soybean. Pustule production in soybean leaves caused by Xanthomonas axonopodis pv. glycines was accompanied by a drastic increase in IAA content of host tissues. The phytopathogenic bacterium synthesized IAA in a tryptophan concentration-dependent manner when grown in a defined minimal medium. In complex media, however, the pathogen showed no response to tryptophan feeding, implying that the bacterial biosynthetic machinery of IAA is strictly regulated by nutrient availability of its growth environments. The results may suggest that IAA of bacterial origin and tryptophan of plant origin be involved in the process of pustule symptom development in soybean.

• 한국연초학회지
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• 제28권2호
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• pp.158-161
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• 2006

Main tobacco diseases were surveyed on major flue-cured leaf tobacco fields throughout South Korea in 2006. Mosaic caused by potato virus Y and bacterial wilt caused by Ralstonia solanacearum were most severe during harvest season. During last ten years, the damage by tobacco mosaic virus was reduced but the incidence of bacterial wilt increased. These changes of the disease incidences coincide with release time of the tobacco cultivar resistant to the tobacco mosaic virus but susceptible to bacterial wilt pathogen. Wild fire(Pseudomonas syringae pv. tabaci strain ungulate) occurred severely at Kangwon province, though the symptom of wild fire with yellow halo was not observed.

• 식물병연구
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• 제25권2호
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• pp.62-70
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• 2019

The global warming by increased $CO_2$ will effect of plant pathogenic microorganisms and resistance of host plants, and it is expected to affect host-pathogen interactions. This study used Capsicum annuum L. and Xanthomonas euvesicatoria, a pathogenic bacteria of pepper, to investigate interactions between hosts and pathogens in a complex environment with increasedcultivation temperature and drought stress. As a result, the bacterial spot disease of C. annuum L. caused by X. euvesicatoria was $35^{\circ}C$ higher than $25^{\circ}C$. In addition, the effect on water potential on bacterial spot disease was much greater water potential -150 kPa than -30 kPa. The disease progress and severity higher than water potential -30 kPa. This result will useful for understanding interaction with red pepper and X. euvesicatoria under the complex environment with increased cultivation temperature and in water potential -150 kPa drought stress in the future.

• 식물병과 농업
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• 제5권2호
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• pp.69-76
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• 1999

Acidovorax avenae subsp. avenae is the causal pathogen of several hosts including oats corn foxtail millet wheatgrass sugarcane and rice. The pathogen is a seedborne pathogen of rice and known to occur widely in rice growing countries. The pathogen cause inhibition of germination brown stripe on the leaf curling of the leaf sheath and abnormal elongation of the mesocotyl of irce. Bacterial colonies grow slowly and are convex circular and creamy with tan to brown center. The causal baterium is Gram-negative and rod shape with a single polar flagellum Nonfluorescence poly-$\beta$-hydroxybutyrate accumulation and precipitate formation around the colony on the medium are useful in the differentiation of this bacterium from other subspecies of A. avenae as well as nonfluorescent bacteria pathogenic to rice. This bacterium has belonged to the genus of Psdeudomonas but recently was transferred to the new genus Acidovorax on the basis of bacteriological and molecular biological data. However the difference of biochemical characteristics protein profile of the cell and host range among strains should be more clarified. To develop an effective control strategy for this disease understanding of detailed life cycle of the disease ritical environmental factors affecting disease development on each host and relationship to grain discoloration of rice are prerequisite. Although the affected area has been world-widely reported there is on recent progress on the understanding of the bacteriological and ecological characteristics of the causal bacterium and control means of the disease.