Bacterial contaminants in extended boar semen and selection of effective antimicrobials

Bacterial contamination is an unavoidable finding of the semen collection process in boar and can lead in deleterious effects on semen quality and longevity if left uncontrolled. The purpose of this study is to identify the bacteria in extended boar semen and to select the effective antimicrobials to control of the contaminants. Of 116 extended boar semen samples submitted from eight AI centers in Korea, 39 (33.6%) samples were positive for bacterial contamination. Among 39 contaminated semen, most of them (84.6%) were contaminated with one or two bacterial species and there was no significant difference between two age groups $(\leq\;24\;and\;>\;24\;month\;old).$ Stenotrophomonas maltophilia (n = 18) was the most predominant bacterium followed by Elizabethkingia meningoseptica (n = 12), Sphingomonas paucimobilis (n = 12), Myroides spp. (n = 5), Ochrobactrum anthropi (n = 3), and so on. Enrofloxacin (72.9%), florfenicol (72.9%), bacitracin (49.2%) and tylosin (49.2%) showed higher sensitivity compared with penicillin (13.6%) or aminoglycosides (6.8%-18.6%). Brucella spp., Leptospira spp., Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, Mycobacterium tuberculosis complex were not detected in semen by PCR.

돼지 희석정액의 세균오염도 및 유효 항생제 선발