• Title/Summary/Keyword: BL32

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Production of Soluble Recombinant Human Granulocyte Colony Stimulating Factor in E. coli by Control of Growth Rate. (대장균에서 증식속도 조절에 의한 수용성 재조합 인간 과립구 콜로니 촉진인자의 생산)

  • 박세철;고인영;강희일
    • Microbiology and Biotechnology Letters
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    • v.32 no.2
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    • pp.135-141
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    • 2004
  • Human granulocyte colony-stimulating factor (hG-CSF) is a hematopoiesis agent that principally affects the differentiation of neutrophils in the bone marrow. At present, recombinant hG-CSF is used successfully in the treatment of chemotheraphy-induced neutropenia and its indication has been expanded to bone marrow transplantation and aplastic anemia. In this study, we have constructed rhG-CSF secretion plasmid pYRC1 in which OmpA signal sequence/hG-CSF gene was expressed under the control of the T7 promoter. rhG-CSF produced in E. coli BL21 (pYRC1) grown at $37{\circ}C$ was found in aggregates. However, 15% of the periplasmic protein was soluble rhG-CSF when the E. coli BL21 (pYRC1) was cultured at $25^{\circ}C$ for 7 h in the modified MBL medium containing 10 g/$\ell$ glucose with 10 $\mu$M IPTG induction. The production of soluble rhG-CSF in E. coli BL21 (pYRC1) using fed batch culture was also studied. In the fed batch culture system, the final yield of rhG-CSF produced from E. coli BL21 (pYRC1) was increased from 4.4 mg/$\ell$to 24 mg/$\ell$by controlling the specific growth rate from $0.43 h^{-1}$ to $0.14 h^{-1}$, and optimizing the time of induction.

Inhibitory Effects of Constituents of Gastrodia elata Bl. on Glutamate-Induced Apoptosis in MIR-32 Human Neuroblastoma Cells

  • Lee, Yong-Soo;Ha, Jeoung-Hee;Yong, Chul-Soon;Lee, Dong-Ung;Huh, Keun;Kang, Young-Shin;Lee, Sun-Hee;Jung, Mi-Wha;Kim, Jung-Ae
    • Archives of Pharmacal Research
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    • v.22 no.4
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    • pp.404-409
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    • 1999
  • The inhibitory effects of the constituents of Gastrodia elata Bl. (GE) on glutamate-induced apoptosis in human neuronal cells were investigated using IMR32 human neuroblastoma cells. Glutamate (GLU) induced DNA fragmentation, a hallmark of apoptosis, in a dose-dependent manner. GLU also induced a slow and sustained increase in intracellular $Ca^{2+}$ concentration. Treatment with EGTA, an extracellular $Ca^{2+}$ chelator, in a nominal $Ca^{2+}$ -free buffer solution abolished the GLU-induced intracellular $Ca^{2+}$ increase, indicating that GLU stimulated Ca2+ influx pathway in the IMR32 cells. BAPTA, an intracellualr $Ca^{2+}$ chelator, significantly inhibited the GLU-induced apoptosis assessed by the flow cytometry measuring hypodiploid DNA content indicative of apoptosis, implying that intracellular $Ca^{2+}$ rise may mediate the apoptotic action of GLU. Vanillin (VAN) and p-hydroxybenzaldehyde(p-HB), known constituents of GE, significantly inhibited both intracellular $Ca^{2+}$ rise and apoptosis induced by GLU. These results suggest that the apoptosis-inhibitory actions of the constituents of GE may account, at least in part, for the basis of their antiepileptic activities. These results further suggest that intracelluarl $Ca^{2+}$ signaling pathway may be a molecular target of the constituents of GE.

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A Study on Digital Infrared Thermographic Imagic Characters of women suffering from Postpartum disease (DITI를 이용한 산후풍 환자의 체표 온도 특성 연구)

  • Park, Kyoung-Sun;Lee, Yoon-Jae;Hwang, Deok-Sang;Lee, Jin-Moo;Lee, Chang-Hoon;Cho, Jung-Hoon;Jang, Jun-Bock;Lee, Kyung-Sub
    • Journal of Oriental Medical Thermology
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    • v.6 no.1
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    • pp.49-55
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    • 2008
  • Purpose: During Postpartum period many women complain multiple pain, cold hypersensitivity, hot flush, sweating and so on. We call the postpartum disease as San Hu Pung. We studied characters of DITI Characters of women suffering from Postpartum disease. Methods: We studied 55 patients visiting OOhospital from February 2006 to November 2007. The subjects were categorized in two groups, symptom group(37) and no symptom group(18). We measured the temperatures of abdomen(CV17, CV12, CV4), upper and lower limb(PC8, LU4, LR3, ST32) and back(GB21, BL18, BL53). We studied the difference of DITI between two groups by Student T-test using SPSS for windows (version 12.0). Results: The general characteristics such as age, days of postpartum, primiparity, cesarean section, partum season, breast-feeding of two groups were not different statistically. Temperatures of abdomen(CV17, CV12, CV4) and back(GB21, BL18, BL53) of two groups were not different statistically. Temperatures of Lt. PC8 and both LR3 of symptom group were statistically higher than no symptom group. The difference between Lt. PC8 and LU4, both LR3 and ST32 of symptom group were statistically higher than no symptom group. Conclusion: The results suggest that women suffering from Postpartum disease shows high temperature on hand and foot. It seems that postpartum disease patients tend to have blood deficiency. DITI can be useful to diagnose San Hu Pung. The more studies to diagnose San Hu Pung would be needed.

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Antioxidant and Anti-Obesity Effect of SM17 in High-Fat Diet Induced C57BL/6 Mice (고지방식이로 비만을 유도한 C57BL/6 마우스에서 SM17의 항산화 및 항비만 효과)

  • Kim, Soo Hyun;Kim, Su Ji;Kim, Kyeong Jo;Lee, Ah Reum;Roh, Seong-Soo;Lee, Young Cheol
    • The Korea Journal of Herbology
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    • v.32 no.5
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    • pp.47-55
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    • 2017
  • Objectives : Obesity is caused by the excess accumulation of fat in the body due to energy imbalance, and it causes various diseases. The aim of this study was to investigate an anti-obesity efficacy and an antioxidant activity of water from herbal mixture extract (SM17). Methods : The antioxidant activities were evaluated through radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals. To evaluated anti-obesity effect of SM17, we used a high fat diet fed mouse model. The SM17 (150 mg/kg body weight/day, p.o.) was treated every day for 6 weeks to C57BL/6 mice. Body weight and food intake were measured every day. The changes of reactive oxygen species (ROS), alanine aminotransferanse (ALT), aspartate aminotransferase (AST), triglycerids (TG) and total cholesterol (TC) in serum were analyzed after experiment. Also, expression of lipid metabolism related proteins were investigated by western blot analysis. Results : It was effective in antioxidant measurements, SM17 administration inhibited the biomarkers of lipid metaboism in serum and tissues. The administration of SM17 showed a significant reduction of body and tissue weight. Morever, it decreased ROS, ALT, AST, TG and TC in serum, compared with those of the obese mice. Adipogenesis-related protein expressions increased in obese mice compared to normal mice. However, SM17 group exhibited the down-expression of these proteins. Conclusion : A SM17 aqueous extract has a great effect on the stimulation (AMPK) activation, and may have a benefit to reduce a fatty acid metabolism through inhibition of lipid accumulation.

Beneficial Effects of Fermented Cricket Powder as a Hair Growth Promoting Agent in a Mice Model (생쥐 모델에서 쌍별 귀뚜라미 발효 분말의 발모 촉진제로서의 유익한 효과)

  • Hwang, Jihye;Hwang, Ui Wook
    • Journal of Life Science
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    • v.32 no.3
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    • pp.196-201
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    • 2022
  • Insects have been proposed as new protein sources for human nutrition, and protein availability is affected by insect characteristics. Fermentation can be used to obtain a variety of insect-based ingredients and products with unique properties, but its effect on protein availability is unknown. Fermented cricket (Gryllus bimaculatus) powder consists mainly of protein, and its oral administration has been reported to improve hair growth in androgenetic alopecia. The purpose of this study was to evaluate the hair-promoting activity of fermented cricket powder in an animal model using male C57BL/6 mice (25-30 g). The abdominal hair of the mouse (2x2.5 cm) was gently removed, and the groups fed as follows: Intact controls (no cricket powder); cricket powder only; and fermented cricket powder only. Food was applied daily for 11 weeks. Observational and physical examinations were performed and the results of the different groups compared. The application of fermented cricket powder significantly (p<0.01) promoted hair growth compared to the intact controls. The C57BL/6 results confirmed increased growth after seven weeks when the proportion of anagen follicles had increased by about 125% and 120% in the control and cricket powder groups, respectively. In conclusion, fermented cricket powder can be seen as a promising alternative alopecia treatment because it promotes hair growth, and, given the powder's composition, trace elements such as amino acids may have contributed to these effects.

Design of eFuse OTP Memory with Wide Operating Voltage Range for PMICs (PMIC용 넓은 동작전압 영역을 갖는 eFuse OTP 설계)

  • Jeong, Woo-Young;Hao, Wen-Chao;Ha, Pan-Bong;Kim, Young-Hee
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.18 no.1
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    • pp.115-122
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    • 2014
  • In this paper, reliability is secured by sensing a post-program resistance of several tens of kilo ohms and restricting a read current flowing over an unblown eFuse within $100{\mu}A$ since RWL driver and BL pull-up load circuits using a regulated voltage of V2V ($=2V{\pm}10%$) are proposed to have a wide operating voltage range for eFuse OTP memory. Also, when a comparison of a cell array of 1 row ${\times}$ 32 columns with that of 4 rows ${\times}$ 8 columns is done, the layout size of 4 rows ${\times}$ 8 columns is smaller with $187.065{\mu}m{\times}94.525{\mu}m$ ($=0.01768mm^2$) than that of 1 row ${\times}$ 32 columns with $735.96{\mu}m{\times}61.605{\mu}m$ ($=0.04534mm^2$).

High-Level Expression of A Bacillus subtilis Mannanase Gene in Escherichia coli. (대장균에서 Bacillus subtilis의 Mannanase 유전자 과잉발현)

  • 권민아;손지영;윤기홍
    • Microbiology and Biotechnology Letters
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    • v.32 no.3
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    • pp.212-217
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    • 2004
  • The gene coding for mannanase from Bacillus subtilis WL-7, a number of glycosyl hydrolase family 26, was hyperexpressed in Escherichia coli. Two recombinant plasmids, pE7MAN and pENS7, were constructed by introducing the complete mannanase gene and the mature mannanase gene lacking N-terminal signal peptide region into a expression vector pET24a(+), respectively. The level of mannanase produced by E. coli BL21 (DE3) carrying pENS7, which included the mature mannanase gene, was considerably higher than that by E. coli BL21 (DE3)/pE7MAN. Almost mannanase produced by the recombinant E. coli carrying pENS7 at growth temperature of $37^{\circ}C$ existed as inactive enzyme of insoluble form. Growth at temperature below $31^{\circ}C$ increased the soluble fraction of mannanase having catalytic activity in the recombinant E. coli cells. The highest productivity of active mannanase was observed in cell-free extract of the recombinant E. coli grown at growth temperature ranging from $25^{\circ}C$ to $28^{\circ}C$, while mannanase activity per soluble protein of the cell-free extract was highest in the cells grown at $^31{\circ}C$.

Modifying Effect of Diallyl Sulfide on Colon Carcinogenesis in C57BL/6J-ApcMin/+ Mice

  • Kang, Jin-Seok;Kim, Tae-Myoung;Shim, Tae-Jin;Salim, Elsayed I.;Han, Beom-Seok;Kim, Dae-Joong
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.4
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    • pp.1115-1118
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    • 2012
  • Diallyl sulfide (DAS), a flavoring compound derived from garlic, is considered to have cancer chemopreventive potential in experimental animals and humans. This study was designated to examine possible chemopreventive effects of DAS on colon carcinogenesis using genetically engineered transgenic $Apc^{Min/+}$ mice, a well-established animal model for familial adenomatous polyposis (FAP) and sporadic colorectal cancer. Male C57BL/6J-$Apc^{Min/+}$ mice were divided into three groups. Animals of group 1 were placed on the basal diet (AIN-76A) as non-treated controls. Animals of groups 2 and 3 were given DAS-containing diets (in doses of 100 and 300 ppm, respectively). All mice were sacrificed at the end of week 10 of the experiment. Histopathological investigation revealed that the incidence of colonic polyps was decreased dose-dependently by 19% (13/16) in group 2 and by 32% (13/20) in group 3 compared to the 100% incidence (10/10) in group 1. The multiplicity of colonic polyps per mouse was also slightly decreased by DAS treatment ($1.88{\pm}0.35$ in group 2 and $1.63{\pm}0.36$ in group 3) compared to $2.00{\pm}0.39$ in group 1. On the other hand, there were no significant differences in the numbers of total polyps per mouse in the small intestine between the groups. Taken together, we suggest that DAS may exert promising inhibitory effects on colon carcinogenesis in the transgenic $Apc^{Min/+}$ mice.

A Philological Study on the Pathology, Acupuncture and Moxibustion of Alopecia (탈발(脫髮)의 병인병기(病因病機) 및 침구치료(鍼灸治療)에 관한 문헌고찰)

  • Lee, Sang Hyun;Cho, Myoung Rae
    • Journal of Acupuncture Research
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    • v.32 no.2
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    • pp.35-57
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    • 2015
  • Objectives : The purpose of this study is to analyze literature on oriental medicine to investigate the pathology, acupuncture, and moxibustion therapy applicable to alopecia Methods : We searched for the causes of alopecia in the bibliographic data from 41 sources of literature and the acupunctural treatments for alopecia in the bibliographic data from 49 sources of Chinese and Korean literature and from the journals of the acupunctural treatments for alopecia which have been published since 2000. Results : 1. The main causes of alopecia are blood deficiency, blood heat, static blood, depletion of kidney's water, wind excess, seven emotional damage, dietary damage, etc. 2. The acupuncture points frequently used are $GV_{20}$, $GB_{20}$, $ST_{36}$, $SP_6$, $SP_{10}$, $BL_{17}$, $BL_{18}$, $BL_{23}$, $GV_{23}$, $GV_{14}$, $CV_6$, $ST_8$, $KI_3$, $LR_3$, $HT_7$, $LI_{11}$, EX-$HN_1$, and ouch point. 3. New acupuncture therapies, including herbal acupuncture therapy, ear acupuncture therapy, dermal needle therapy, needle-embedding therapy and scalp acupuncture therapy are frequently used to treat alopecia. Conclusions : The acupuncture points frequently used for treating alopecia have characteristics such as tonifying blood, tonifying the liver and kidney, extinguishing wind, activating blood, clearing heat and draining dampness, so they are highly associated with the causes and the mechanisms of alopecia.

Effects of Natural Extracts on UVB-induced Pigmentation and Inflammation in C57BL/6 Mouse Skin (천연물 도포가 UVB 파로 손상된 C57BL/6 mouse 피부의 색소침착과 염증생성에 미치는 영향)

  • Choi, Wook-Hee;Ann, Hyoung-Soo;Choi, Tae-Youn;Jin, So-Young;Ahn, Ryoung-Me
    • Journal of Environmental Health Sciences
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    • v.32 no.5 s.92
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    • pp.492-498
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    • 2006
  • Ultraviolet(UV) radiation causes a variety of biological effects on the skin, including inflammation, pigmentation, photoaging and cancer. Free radicals are involved in inflammatory skin reactions induced by UVB radiation. In this study, we investigated the effects of antioxidants(Tea, Korean red ginseng, Ginkgo biloba extract) on UVB-induced skin damage. Tea, KRG and EGb 761 were topically treated to dorsal skin of ICR mouse. The mice were also treated soon after IMED ($1.4KJ/m^{2}$) of UVB irradiation. Skin pigmentation of irradiated mouse was observed by a chromameter after 2 weeks. Topical application of Tea, KRG and EGb 761 for 2 weeks decreased skin pigmentation compared to DVB control group(p<.05). Tea, KRG and EGb 761 also reduced UVB-induced infiltration of inflammatory cells. These results showed that Tea, KRG and EGb 761 as a topical application may have preventive effect against UVB-induced skin damage.