The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Antioxidant and Anti-Obesity Effect of SM17 in High-Fat Diet Induced C57BL/6 Mice

고지방식이로 비만을 유도한 C57BL/6 마우스에서 SM17의 항산화 및 항비만 효과

  • Kim, Soo Hyun (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Su Ji (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Kim, Kyeong Jo (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Ah Reum (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Young Cheol (Department of Herbology, College of Korean Medicine, Sangji University)
  • 김수현 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김수지 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김경조 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이아름 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 이영철 (상지대학교 한의과대학 본초학교실)
  • Received : 2017.08.12
  • Accepted : 2017.09.15
  • Published : 2017.09.30
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Abstract

Objectives : Obesity is caused by the excess accumulation of fat in the body due to energy imbalance, and it causes various diseases. The aim of this study was to investigate an anti-obesity efficacy and an antioxidant activity of water from herbal mixture extract (SM17). Methods : The antioxidant activities were evaluated through radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals. To evaluated anti-obesity effect of SM17, we used a high fat diet fed mouse model. The SM17 (150 mg/kg body weight/day, p.o.) was treated every day for 6 weeks to C57BL/6 mice. Body weight and food intake were measured every day. The changes of reactive oxygen species (ROS), alanine aminotransferanse (ALT), aspartate aminotransferase (AST), triglycerids (TG) and total cholesterol (TC) in serum were analyzed after experiment. Also, expression of lipid metabolism related proteins were investigated by western blot analysis. Results : It was effective in antioxidant measurements, SM17 administration inhibited the biomarkers of lipid metaboism in serum and tissues. The administration of SM17 showed a significant reduction of body and tissue weight. Morever, it decreased ROS, ALT, AST, TG and TC in serum, compared with those of the obese mice. Adipogenesis-related protein expressions increased in obese mice compared to normal mice. However, SM17 group exhibited the down-expression of these proteins. Conclusion : A SM17 aqueous extract has a great effect on the stimulation (AMPK) activation, and may have a benefit to reduce a fatty acid metabolism through inhibition of lipid accumulation.

Keywords

References

  1. Chua SC, Leilbel RL. Obesity genes: Molecular and metabolic mechanisms. Diabetes Reviews. 1997 ; 5(1) : 2-7.
  2. Kim SW, Kim NS, Oh MJ, Kim HR, Kim MS, Lee DY, Yoon SH, Jung MY, Kim HJ , Lee CH, Oh CH. Anti-Obesity Effects of Fermented Soybean Oils in 3T3-L1 Pre-Adipocytes and High Fat Diet-Fed C57BL/6J Mice. J Korean Soc Food Sci Nutr. 2017 ; 46(3) : 279-88. https://doi.org/10.3746/jkfn.2017.46.3.279
  3. Lee DS, Lee MH, Kim HS, Jeong TR, Yang HP, Hyun HS, Lee JM. Anti-Obesity Effect of Pine Cone (Pinus koraiensis) Supercritical Extract in High-Fat Diet-Induced Obese Mice. J Korean Soc Food Sci Nutr. 2016 ; 45(12) : 1701-7. https://doi.org/10.3746/jkfn.2016.45.12.1701
  4. Baek SJ, Kim DH. The Study on anti-obesity of Myrrh ethanol extract. Kor. J. Herbol. 2016 ; 31(4) : 11-8. https://doi.org/10.6116/KJH.2016.31.4.11.
  5. Jung YM, Ku SK, Lee DS, Kwon Ks. Lactobacillus plantarum-fermented Opuntia humifusa Extracts (fOH) Increases the Anti-obesity Activity in Mice Fed a 45% Kcal High Fat Diet. Journal of Life Science. 2017 ; 27(6) : 680-7. https://doi.org/10.5352/JLS.2017.27.6.680
  6. Kim JY, Shin MS, Heo YR. Effects of Stabilized Rice Bran on Obesity and Antioxidative Enzyme Activity in High Fat Diet-induced Obese C57BL/6 Mice. J Korean Soc Food Sci Nutr. 2014 ; 43(8) : 1148-57. https://doi.org/10.3746/jkfn.2014.43.8.1148
  7. Halliwill B. Antioxidant in human health and disease. Annu Rev Nutr. 1996 ; 16(1) : 33-50. https://doi.org/10.1146/annurev.nu.16.070196.000341
  8. Bae GJ, Ha BJ. Antioxidative Effect of Fermented Rhynchosia nulubilis in Obese Rats. J. Food Hyg. Saf. 2015 ; 30(4) : 383-9. https://doi.org/10.13103/JFHS.2015.30.4.383
  9. Park JE, Shin EJ, Ha BG, So MS, Shon YH. Effect of Deep Sea Water containing Chitosan Oligosaccharides on Fatty Acids Synthesis, ${\beta}$-oxidation, and AMPK Activation. J. Chitin Chitosan. 2013 ; 18(2) : 93-8.
  10. Shon MS, Kim RH, Song JH, Kwon OJ, Lee AR, Kim HO, Roh SS, Kim GN. Potential of Fisetin as a Nutri-cosmetics Material through Evaluating Anti-oxidant and Anti-adipogenic Activities. Asian J Beauty Cosmetol. 2016 ; 14(1) : 6-17. https://doi.org/10.20402/ajbc.2016.0003
  11. Yoon YS. Effects of Cheongshimyeonja-tang on Body Weight and Serum Lipid Level in High Fat Diet Induced Obese Mice. Wonkwang University. 2011 : 1-67.
  12. Park JA, Jin KS, Lee JY, Kwon HJ, Kim BW. Anti-Oxidative and Anti-Obesity Activities of Tetrapanax papyriferus and Siegesbeckia pubescens Extracts and their Synergistic Anti-Obesity Effects. Korean J. Microbiol. Biotechnol. 2013 ; 41(3) : 341-9. https://doi.org/10.4014/kjmb.1306.06002
  13. Hwang CR, Kang MJ, Shim HJ, Suh HJ, Kwon OO, Shin JH. Antioxidant and Antiobesity Activities of Various Color Resources Extracted from Natural Plants. J Korean Soc Food Sci Nutr. 2015 ; 44(2) : 165-72. https://doi.org/10.3746/jkfn.2015.44.2.165
  14. Seo CS, Kim JH, Shin HK, Kim BS. Quantitative Analysis of (+)-Catechin, Paeoniflorin, and Paeonol in Moutan Radicis Cortex and Its Processed Products. Kor. J. Pharmacogn. 2016 ; 47(3) : 237-45.
  15. Bray MS. Genomics, genes, and environmental interaction: the role of exercise. J Appl Physiol. 1985 ; 88(2) : 788-92.
  16. Jeong HS. Efficacy of Alismatis Orientale Rhizoma on Obesity induced by High Fat Diet. Kor. J. Herbology. 2013 ; 28(3) : 95-106. https://doi.org/10.6116/kjh.2013.28.3.95
  17. Kim BH, Seo BI, Lee ES, Kim MR, Shin SS. The effects of Aurantii Fructus Immaturus and Persicae Semen on an obesity of rats fed high fat diet. Kor. J. Herbology. 2003 ; 18(3) : 69-78.
  18. Shin MR, An HJ, Lee YC, Seo BI, Roh SS. Comparative Evaluation of Korean Medicine well-matched with Chicken through an inhibition of Pancreatic Lipase. Kor. J. Herbol. 2017 ; 32(4) : 9-15. https://doi.org/10.6116/KJH.2017.32.4.9
  19. Kim SH, Lee AR, Kim SJ, Kim KJ, Kwon OJ, Choi JY, Koo JS, Roh SS. Skin elasticity improvement effect of Young persimon and Heated young persimon by decreased Advanced glycation end products(AGEs). Kor. J. Herbology. 2017 ; 32(4) : 17-24.
  20. Shin YJ, Jeon SY. Anti-hyperlipidemic and Antioxidant effects of Kgihwaetang-gamibang in High Cholesterol Diet-induced Mice Model. J Physiol & Pathol Korean Med. 2015 ; 29(5) : 422-33. https://doi.org/10.15188/kjopp.2015.10.29.5.422
  21. Lee SI, Lee YK, Kim SD, Lee IA, Choi JK, Suh JW. Dietary Effects of Fermented Soybean Curd Residue (Biji) on Body Weight, Serum Lipid Profiles, and Antioxidation-Related Enzymes Activity of Mice Fed a High Fat Diet. J Korean Soc Food Sci Nutr. 2013 ; 42(7) : 1043-53. https://doi.org/10.3746/jkfn.2013.42.7.1043
  22. Schaefer EJ. Lipoproteins, nutriton, aging and atherosclerosis. Am J Clin Nutr. 1995 ; 61(3) : 726-40. https://doi.org/10.1093/ajcn/61.3.726S
  23. Bae GJ, Ha BJ. Effects of Cladosiphon okamuranus on Lipid Metabolism in High-fat-diet Rats. Journal of Life Science. 2016 ; 26(6) : 657-62. https://doi.org/10.5352/JLS.2016.26.6.657
  24. Choi HM, Moon SO, Lee HH, Lee HD. Inhibitory effect of by Ojeok-san lipid accumulation in high fat diet-induced obesity mice and 3T3-L1 adipocytes. Kor. J. Herbol. 2015 ; 30(4) : 121-8. https://doi.org/10.6116/kjh.2015.30.4.121.
  25. Lee Jin. Effect of Swimming Training on Hepatic SREBP-1c, ACC and AMPK in Aging Mice. Korean Journal of Sport Science. 2010 ; 21(1) : 938-46. https://doi.org/10.24985/kjss.2010.21.1.938