• Title/Summary/Keyword: B16F10 cell lines

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The Effects of Growth Inhibition and Quinone Reductase Activity Stimulation of Celastrus Orbiculatus Fractions in Various Cancer Cells (노박덩굴 분획물의 암세포 증식 억제 효과 및 Quinone Reductase 활성 증가효과)

  • Ku, Mi-Jeong;Shin, Mi-Ok
    • Journal of Nutrition and Health
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    • v.40 no.6
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    • pp.493-499
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    • 2007
  • Celastrus orbiculatus (CO) has been used as a traditional herb medicine to treat fever, chill, joint pain, edema, rheumatoid arthritis and bacterial infection in China and Korea. In this study, we investigated anticarcinogenic effects of Celastrus orbiculatus (CO). CO was extracted with methanol (COM), and then further fractionated into four different types: methanol (COMM), hexane (COMH), butanol (COMB) and aqueous (COMA) partition layers. We determined the cytotoxicity of these four partitions in four kind of cancer cell lines, such as HepG2, MCF-7, HT29 and B16F10 Cells by MTT assay. Among various partition layers of CO, the COMM showed the strongest cytotoxic effects on cancer cell lines we used. We also observed quinone reductase (QR) induced effects in all partition layers of CO on HepG2 cells. The QR induced effects of COMM on HepG2 cells at 80 ${\mu}$ g/mL concentration indicated 3.28 to a control value of 1.0. The COMM showed the highest induction activity of quinone reductase on HepG2 cells among the other partition layers. Although further studies are needed, the present work suggests that CO may be a chemopreventive agent for the treatment of human cells.

Effect of Growth Inhibition and Quinone Reductase Activity Stimulation of Makgeoly Fractions in Various Cancer Cells (막걸리 분획물에 의한 암세포 성장 억제 및 Quinone Reductase 활성 증가 효과)

  • Shin, Mi-Ok;Kang, Dae-Yeon;Kim, Mi-Hyang;Bae, Song-Ja
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.3
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    • pp.288-293
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    • 2008
  • In this study, we investigated the anticancer activity of Makgeoly (MG). MG was fractionated into four fractions by using solvent partition method, affording hexane (MGH), methanol (MGM), butanol (MGB) and aquous (MGA) soluble fractions. We determined the cytotoxicity of these four fractions in four kinds of cancer cell lines, such as HepG2, MCF-7, B16-F10 and HT29 by MTT assay. Among the various fractions, the MGM showed the strongest cytotoxic effects on all cancer cell lines. The morphological changes such as membrane shrinking and blebbing of cells were also observed by MGM treatment in HepG2 cell. In addition, we observed quinone reductase (QR) activity stimulating effects in all fraction layers of MG on HepG2 cells. QR activity increased approximately 2.6 and 2.1 times in MGM and MGH treated HepG2 cell at $100{\mu}g/mL$, respectively, compared to that in control value. Although further studies are needed, the present work could suggest that the fin of MG has a potential to be used as a chemopreventive agent against cancer.

Production of the Rare Ginsenoside Rh2-MIX (20(S)-Rh2, 20(R)-Rh2, Rk2, and Rh3) by Enzymatic Conversion Combined with Acid Treatment and Evaluation of Its Anti-Cancer Activity

  • Song, Bong-Kyu;Kim, Kyeng Min;Choi, Kang-Duk;Im, Wan-Taek
    • Journal of Microbiology and Biotechnology
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    • v.27 no.7
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    • pp.1233-1241
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    • 2017
  • The ginsenoside Rh2 has strong anti-cancer, anti-inflammatory, and anti-diabetic effects. However, the application of ginsenoside Rh2 is restricted because of the small amounts found in Korean white and red ginsengs. To enhance the production of ginsenoside Rh2-MIX (comprising 20(S)-Rh2, 20(R)-Rh2, Rk2, and Rh3 as a 10-g unit) with high specificity, yield, and purity, a new combination of enzymatic conversion using the commercial enzyme Viscozyme L followed by acid treatment was developed. Viscozyme L treatment at pH 5.0 and $50^{\circ}C$ was used initially to transform the major ginsenosides Rb1, Rb2, Rc, and Rd into ginsenoside F2, followed by acid-heat treatment using citric acid 2% (w/v) at pH 2.0 and $121^{\circ}C$ for 15 min. Scale-up production in a 10-L jar fermenter, using 60 g of the protopanaxadiol-type ginsenoside mixture from ginseng roots, produced 24 g of ginsenoside Rh2-MIX. Using 2 g of Rh2-MIX, 131 mg of 20(S)-Rh2, 58 mg of 20(R)-Rh2, 47 mg of Rk2, and 26 mg of Rh3 were obtained at over 98% chromatographic purity. Then, the anti-cancer effect of the four purified ginsenosides was investigated on B16F10, MDA-MB-231, and HuH-7 cell lines. As a result, these four rare ginsenosides markedly inhibited the growth of the cancer cell lines. These results suggested that rare ginsenoside Rh2-MIX could be exploited to prepare an anti-cancer supplement in the functional food and pharmaceutical industries.

Study on Antitumor and Antimetastatic Effect of Kamigedang-tang (가미저당탕의 항암 및 항전이 효과에 관한 연구)

  • Lee Dong Hoon;Kim Dong Hee;Kang In Cheol;Park Young Mi;Song Gyu Yong;Kim Sung Hoon
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.16 no.3
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    • pp.472-478
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    • 2002
  • The purpose of this study was to investigate the effect of Kamigedang-tang(KGDT) water extract on the antitumor and anti metastatic activity. The results were summarized as follows: KGDT extracts exhibited a significant cytotoxicity against P388, SK-MEL-2, SK-OV-3, and B16-F10 cell lines and showed significant inhibitoty effect on DNA topoisomerase I from calf thymus. The T/C% was 122.9% in KGDT treated group in S-180 bearing ICR mice. Also, KGDT extracts exhibited efficient affect adhesive effect of A549 cell to complex extracellular matrix. In CAM assay, KGDT extracts inhibited angiogenesis at 15㎍/egg concentration insignificantly as compared with control. These results suggested that KGDT extracts might be usefully applied for prevention and treatement of cancer.

Study on Antitumor Activity and Antimetastatic effect of Kamigumguesingihwan(KGSH) (가미금궤신기환(加味金櫃腎氣丸)의 항암(抗癌) 및 항전이(抗轉移) 효과(效果)에 관(關)한 연구(硏究))

  • Kim, Yong-Tae;Jeon, Young-Soo;Kim, Jung-Hyo;Kim, Sung-Hoon
    • THE JOURNAL OF KOREAN ORIENTAL ONCOLOGY
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    • v.5 no.1
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    • pp.19-32
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    • 1999
  • To evaluate the antitumor activity and antimetastatic effects of Kamigumgusingihwan(KGSH) studies have ken done. The results were obtained as follows: 1. KGSH extracts exhibited a weak cytotoxicity against A549, SK-OV-3, B16-F10, and SK-MEL-2 cell lines. But exhibited potent cytotoxicity against P388 cell line in a dose-dependent manner. 2. The concentration inhibiting adhesion of A549, to complex extracellular matrix up to below 30% of control was recognized at $10^{-3}g/ml$ of KGSH 3. KGSH extracts showed a weak inhibitoty effect on DNA topo-isomerase I from calf thymus. 4. The T/C% was 137% in KGSH treated group in S-180 bearing ICR mice. 5. In pulmonary colonization assay, a number of colonies in the lungs were decreased significantly in KGSH treated group as compared with control group. 6. In hematological changes in B16-BL6 injected C57BL/6, numbers of WBC were decreased insignificantly in KGSH treated groups, and also those of platelet were increased insignificantly in KGSH treated groups as compared with control. 7. In CAM assay, KGSH extracts inhibited angiogenesis at $15{\mu}g/egg $concentration significantly as compared with control. Taken together these results, it is strongly demonstrated that KGSH significantly suppressed tumor metastasis by blocking cell adhesion to extracellular matrix. Therefore, KGSH is expected to be clinically a potent antimetastatic drug for the prevention and treatment of cancer.

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Cytotoxicity and Quantitative Analysis of Nortriterpenoid glycoside from Scilla scilloides (무릇에서 분리한 nortriterpenoid glycoside의 암세포에 대한 세포독성 및 함량 분석)

  • Lee, Sang-Myung;Chun, Hyo-Kon;Lee, Choong-Hwan;Lee, Ho-Jae;Kang, Shin-Jyung;Maeng, Hack-Young;Kho, Yung-Hee
    • Korean Journal of Pharmacognosy
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    • v.32 no.3 s.126
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    • pp.189-192
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    • 2001
  • Scillascilloside E-3 (1) and E-1 (2) were isolated from the bulbs of Scilla scilloides. The cytotoxic activities of these compounds were tested against murine (B16/F-10, 3LL) and human cancer cell lines (MCF7, PC-3, HT29, LOX-IMVI, A549 and HT1080). These compounds exhibited a significant cytotoxic activities against all tested cancer cells. Futhermore, the contents of 1 and 2 in S. scilloides are 43.2 and 27.9 mg/kg, respectively.

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Skin Whitening Effect of Leonuri Herba Extracts(LHE) on Malignant Melanoma Cell (익모초(益母草) 추출물이 악성 흑색종 세포에 미치는 피부미백효과)

  • Kim, Ji-Eun;Lim, Kyeong-Min;Na, Ga-yeong;Kim, Hye-Hwa;Kang, Byung-Soo;Choi, Jung-Hwa;Park, Soo-Yeon;Jung, Min-Yeong
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.30 no.4
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    • pp.49-61
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    • 2017
  • Objectives : In order to find out the whitening effects of Leonuri Herba, this study was designed to identify the effects and the action mechanism of LHE(Leonuri Herba extract) on Malignant melanoma cell lines. Methods : After treating LHE on the B16F10 cell-Malignant melanoma cell line-, the cell survival rate, melanin biosynthesis rate, intra&extracellular tyrosinase activity rate, SOD-like activity, tyrosinase mRNA gene expression were investigated. The results were compared with control group without LHE treatment or with positive control group treated with whitening efficacy substance. Results : B16F10 cell survival rate, melanin biosynthesis rate, and intra&extracellular tyrosinase activity were significantly inhibited depending on the concentration of treated LHE. Melanin biosynthesis rate and tyrosinase activity rate were also decreased when ${\alpha}-MSH$ was combined with LHE. In addition, the SOD-like activity was increased in a concentration-dependent manner in the treatment with the LHE, indicating signigicant activity at high concentrations, and the tyrosinase mRNA gene expression was decreased in both the LHE-treated group, the LHE and ${\alpha}-MSH-treated$ group. Conclusions : LHE seems to inhibit melanin synthesis through inhibition of tyrosinase activity and inhibition of tyrosinase mRNA gene expression. It also has the effect of promoting SOD-like activity and may be used clinically as a skin whitening agent in the future.

Fermentative Characteristics and Anti-Proliferative Activity against Mouse Carcinoma Cell Line of Kimchi prepared with Functional Cabbage (기능성 배추 김치의 발효 특성과 암세포 증식저해능)

  • Yu, Kwang-Won;Lee, Seong-Hyun;Shin, Eun-Hae;Hwang, Jong-Hyun
    • The Korean Journal of Food And Nutrition
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    • v.30 no.5
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    • pp.1007-1014
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    • 2017
  • To compare functional Chinese cabbage('Amtak' baechu; F1 hybrid cultivar between Brassica rapa and B. perkinensis, AB) with general Chinese cabbage ('Chunkwang' baechu; general spring cultivar, CB), two kinds of kimchi(ABK and CBK) prepared with AB and CB cultivar were fermented at $10^{\circ}C$ for 10 days. Their fermentative characteristics and anti-proliferative activities against mouse carcinoma cell lines were investigated. General kimchi(CBK) showed mature pH on the $6^{th}$ day of fermentation, whereas functional kimchi(ABK) reached pH on the $9^{th}$ day. CBK also exhibited acidity of mature stage on the $6^{th}$ day, but ABK reached mature acidity on the $9^{th}$ day. Although ABK and CBK were salted in the same condition, ABK had lower salinity than CBK, throughout the fermentation time. The highest total bacterial and lactic bacterial counts of CBK showed on the $8^{th}$ day of fermentation, but ABK showed the highest total bacterial and lactic bacterial counts on the $10^{th}$ day. The texture of ABK was harder than CBK for fermentation time. This seems to be corrleated with the slower fermentation rate of ABK. ABK showed significantly higher anti-proliferative activity (54.6% cell viability of control) in B16BL6 at $1,000{\mu}g/mL$. ABK was also higher in anti-proliferative activity than CBK throughout the fermentation time. However, there was no significant difference in the anti-proliferative activity of ABK between the fermentation times. In conclusion, fermentation of ABK showed a better texture, due to the slow fermentation rate and more anti-proliferative activity against mouse carcinoma cell line than those of CBK.

Anti-oxidative and Whitening Efects of 4'-O-methylalpinumisoflavone Isolated from Fruit of Maclura Tricuspidata Carrière (꾸지뽕나무 열매에서 추출한 4'-O-methylalpinumisoflavone의 항산화 및 미백 효과)

  • Lyu, Ji Hyo;Noh, Joon Yong;Kim, Sura;Lee, Guem San;Lee, Dongho;Kim, Koanhoi;Kim, Hyungwoo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.33 no.6
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    • pp.349-355
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    • 2019
  • The anti-inflammatory effects of 4'-O-methylalpinumisoflavone (OMAI) has been reported in recent years. To develop effective and safe skin whitening agents, we investigated the anti-oxidative and melanogenic effects of OMAI isolated from fruit of Maclura tricuspidata Carrière (Cudrania tricuspidata) in macrophage and melanoma cell lines. In our results, OMAI showed effective superoxide scavenging activity and suppressed production of lipopolysaccharide (LPS)-induced intracellular reactive oxygen species (ROS) in RAW264.7 cells. In addition, α-melanocyte stimulation hormone (MSH)-induced production of melanin was also reduced by OMAI in B16F10 cells. Finally, OMAI significantly inhibited tyrosinase activity in B16F10 cells. These results suggest that OMAI suppressed melanin production via scavenging reactive oxygen species and inhibition of tyrosinase activity.

Potential uses of Aristotelia chilensis extracts as novel cosmetic materials (마키베리 추출물의 화장품 신규 원료로서의 가능성)

  • Kim, Mijung;Park, Seyeon
    • Journal of Applied Biological Chemistry
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    • v.62 no.4
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    • pp.339-345
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    • 2019
  • The present study aims to investigate the potential applications of Aristotelia chilensis (A. chilensis) extracts as novel cosmetic materials. The total extracts of A. chilensis were partitioned into chloroform (CHCl3), ethyl acetate (EtOAc), and distilled water (DW) fractions. A. chilensis extracts exhibited no cytotoxicity toward HaCaT human keratinocyte and B16F10 mouse melanoma cell lines. CHCl3, EtOAc, and DW extracts reduced oxidative stress, and EtOAc extract was superior to glutathione, a natural human antioxidant positive control. The extracts of A. chilensis reduced melanin synthesis in cells treated with α-melanocyte-stimulating hormone. The extracts of A. chilensis exhibited antibacterial effects toward Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), and Pseudomonas aeruginosa (P. aeruginosa). In particular, the EtOAc extract was effective in terms of antibacterial activity against S. aureus. In the present study, we identified several potential applications of A. chilensis extracts in terms of novel antioxidant and whitening cosmetic materials as well as antibacterial preservatives.