THE JOURNAL OF KOREAN ORIENTAL ONCOLOGY (대한한방종양학회지)

Korean Association of Traditional Oncology (대한암한의학회)
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Study on Antitumor Activity and Antimetastatic effect of Kamigumguesingihwan(KGSH)

가미금궤신기환(加味金櫃腎氣丸)의 항암(抗癌) 및 항전이(抗轉移) 효과(效果)에 관(關)한 연구(硏究)

  • Kim, Yong-Tae (Dept. of Oncology, Graduate School of Est-West Medical Science, Kyung Hee University) ;
  • Jeon, Young-Soo (College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Jung-Hyo (Nursing College of Cho Sun University) ;
  • Kim, Sung-Hoon (Dept. of Oncology, Graduate School of Est-West Medical Science, Kyung Hee University)
  • 김용태 (경희대학교 동서의학대학원 종양연구팀) ;
  • 전영수 (경희대학교 한의과대학 병리학교실) ;
  • 김정효 (조선대학교 간호대학) ;
  • 김성훈 (경희대학교 동서의학대학원 종양연구팀)
  • Published : 1999.12.30
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Abstract

To evaluate the antitumor activity and antimetastatic effects of Kamigumgusingihwan(KGSH) studies have ken done. The results were obtained as follows: 1. KGSH extracts exhibited a weak cytotoxicity against A549, SK-OV-3, B16-F10, and SK-MEL-2 cell lines. But exhibited potent cytotoxicity against P388 cell line in a dose-dependent manner. 2. The concentration inhibiting adhesion of A549, to complex extracellular matrix up to below 30% of control was recognized at $10^{-3}g/ml$ of KGSH 3. KGSH extracts showed a weak inhibitoty effect on DNA topo-isomerase I from calf thymus. 4. The T/C% was 137% in KGSH treated group in S-180 bearing ICR mice. 5. In pulmonary colonization assay, a number of colonies in the lungs were decreased significantly in KGSH treated group as compared with control group. 6. In hematological changes in B16-BL6 injected C57BL/6, numbers of WBC were decreased insignificantly in KGSH treated groups, and also those of platelet were increased insignificantly in KGSH treated groups as compared with control. 7. In CAM assay, KGSH extracts inhibited angiogenesis at $15{\mu}g/egg $concentration significantly as compared with control. Taken together these results, it is strongly demonstrated that KGSH significantly suppressed tumor metastasis by blocking cell adhesion to extracellular matrix. Therefore, KGSH is expected to be clinically a potent antimetastatic drug for the prevention and treatment of cancer.

Keywords

References

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