• 동의생리병리학회지
• /
• 제16권2호
• /
• pp.348-352
• /
• 2002

This study was carried out to evaluate cytotoxic effects of Atractylodes macrocephala Koidz. (A. macrocephala Koidz.) extract on NIH 3T3 fibloblast. SK-MEL-3 (HBT 69) and KB (ATCC No, OCL 17) cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. 10.2 mg/ml Concentration of A. macrocephala Koidz. extracts in SK-MEL-3 showed that their susceptibility (sensitivity) to these compounds decreased in the following order ; adriamycin > H₂O > ethyl acetate > ethyl alcohol > chloroform > n-hexane in SK-MEL-3 cell lines ; 5-FU > H₂O > n-hexane > ethyl acetate > ethyl alcohol > chloroform in KB cell lines. In order to develop an antimicrobial agent, A. macrocephala Koidz. was extracted with solvents. The minimal inhibitory concentrations (MICs) of each solvent extract of A. macrocephala Koidz. against microogranisms were also examined. Antimicrobial activities of ampicillin and ketoconazole as references were compared to those of each solvent extract of A. macrocephala Koidz. The antimicrobial activity of the ethyl acetate soluble extract of A. macrocephala Koidz. had growth inhibition activity against S. mutans and P. putida (MICs. 500 ㎍/ml). These results suggest that the ethyl acetate soluble extract of A. macrocephala Koidz. possessed antitumorous and antimicrobial agents

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2003년도 춘계 학술발표대회
• /
• pp.113-114
• /
• 2003

• 동의생리병리학회지
• /
• 제34권1호
• /
• pp.24-29
• /
• 2020

In this study, we investigated the inhibition effects of mixtures of Atractylodes macrocephala (AM) and Amomum villosum (AV) water extracts on adipocyte differentiation. Treatment with mixtures of AM and AV extracts in a ratio of 3:1 for 24 and 48 hours did not show any cytotoxicity in OP9 cells. Mixtures of AM(3) and AV(1) extracts inhibited adipocyte differentiation, expression of peroxisome proliferator-activated receptor gamma (PPARγ) and CCAT/enhancer-binding protein alpha (C/EBPα), the major transcription factors of differentiation. It also inhibited the expression of lipoprotein lipase (LPL), adipocyte protein 2 (aP2), which are PPARγ-target genes in adipocyte. We also checked the inhibition effects on cell proliferation during the early stage of differentiation by treatment with mixtures of AM(3) and AV(1) extracts. It markedly inhibited adipocyte proliferation after 48 hours, and also the phosphorylation of ERK1/2 and Akt after 10 min or 3 hour. These results identify a possible mechanism of action of mixtures of AM(3) and AV(1) extracts, suggesting that the mixtures of AM(3) and AV(1) extracts-induced inhibition of ERK and Akt phosphorylation suppresses adipogenesis by inhibiting other signaling cascades that include PPARγ and C/EBPα during the process of OP9 adipocyte differentiation.

• 동의생리병리학회지
• /
• 제34권2호
• /
• pp.75-80
• /
• 2020

In this study, we investigated the dose-dependent effects of mixtures of Atractylodes macrocephala (AM) and Amomum villosum (AV) water extracts in a ratio of 3:1 on high fat diet (HFD)-induced obesity model. Oral administration of various concentrations with mixtures of AM and AV extracts in a ratio of 3:1 for 6 weeks inhibited HFD-induced increases of body, liver and epididymal fat weights in a dose-dependent fashions. Those effects may be mediated by decreased expressions of lipogenesis-related genes such as acetyl coA carboxylase (ACC) and fatty acid synthase (FAS) in liver. Also, increase of insulin and decrease of adiponectin in serum by HFD supply were inhibited by three different dosages of mixtures of AM and AV extracts in a ratio of 3:1. HFD supply induced increases of serum total cholesterol, triglyceride and LDL cholesterol. However, hyperlipidemia was significantly decreased in dose-dependent manners by treatment with mixtures of AM and AV extracts. Based on the results of the present study, hypolipidemic and anti-obesity effects by mixtures of AM and AV extracts were found in HFD-induced obesity model. Further clinical investigation is needed to develop anti-obesity therapeutic or preventive agents by using mixtures of AM and AV extracts.

• Korean Journal of Acupuncture
• /
• 제32권3호
• /
• pp.116-123
• /
• 2015

Objectives : The present study was designed to investigate effects and molecular mechanisms of Atractylodes macrocephala Koidzumi extracts(AMK) on the improvement of adipocyte dysfunction induced by TNF-${\alpha}$ in 3T3-L1 adipocytes. We examined whether AMK could directly influence the inflammation and insulin resistance in 3T3-L1 adipocytes. Methods : Potential roles of AMK in the lipolysis, production of inflammatory adipokines and ROS, expression and phosphorylation of ERK, JNK, and $I{\kappa}B{\alpha}$ protein, and expression of $PPAR{\gamma}$ and C/EBP${\alpha}$ were investigated in this study. Results : Our data demonstrated that TNF-${\alpha}$ significantly increased lipolysis, levels of MCP-1, IL-6, and ROS and phosphorylation of ERK, JNK, and $I{\kappa}B{\alpha}$ protein, while TNF-${\alpha}$ reduced the expression of $PPAR{\gamma}$ and C/EBP${\alpha}$ in adipocytes, suggesting that TNF-${\alpha}$ induced a condition with the occurrence of inflammation and insulin resistance. Those alterations induced by TNF-${\alpha}$ were prevented by the treatment of AMK. AMK down-regulated the phosphorylation of ERK, JNK, and $I{\kappa}B{\alpha}$ protein and up-regulated the expression of $PPAR{\gamma}$ and C/EBP${\alpha}$ on TNF-${\alpha}$-induced inflammation and insulin resistance. Conclusions : Thus, our results indicate that AMK can be used to prevent from the TNF-${\alpha}$-induced adipocyte dysfunction through MAPK, $NF{\kappa}B$ and $PPAR{\gamma}$ pathways.

• 한국자원식물학회지
• /
• 제12권4호
• /
• pp.276-281
• /
• 1999

경북 북부지역에서 큰삽주의 1년생 종근 정식적기를 구명하고자 1996년부터 1998년까지 3개년간 수행한 결과는 다음과 같다. 1. 정식시기별 출현일수는 만식할수록 단축되는 경향이었고 정식시기별 지상부 생육은 3월 20일 정식시 초장이 가장 크고, 주당 지상부 생체중 및 건괴근중은 4월 5일 정식이 각각 51.9, 11.5g으로 가장 양호하였다. 2. 10g당 최대 건괴근수량을 보이는 생육일수는 209일이었으며 경북 북부지역의 큰삽주 1년생 종근 정식 적기는 4월 5일부터 4월 10일 사이로 판단되었다. 3. 생육초기 적산온도가 267$^{\circ}C$일 때 출현일수가 14.4일로 가장 짧았으며 전 생육기간 동안의 적산온도는 3$600^{\circ}C$일 때 10a당 건괴근수량이 233kg으로 가장 많았다.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2001년도 춘계 학술대회지
• /
• pp.208-209
• /
• 2001

• 한방비만학회지
• /
• 제17권2호
• /
• pp.119-126
• /
• 2017

Objectives: This study investigated the effects of the mixed herbal extract from Cinnamomum cassia Blume, Atractylodes macrocephala Koidzumi, Pueraria lobata Benth, and Aconitum carmichaeli Debx (CAPA) on obesity and glucose tolerance in obese mice. Methods: Animals were divided in 6 groups, normal diet, high fat diet (HFD), HFD with CAPA 100 mg/kg (CAPA 100), CAPA 300 mg/kg (CAPA 300), and metformin 200 mg/kg or lorcaserin 10 mg/kg as positive controls, and treated for 16 weeks. Body weight, food intake, fasting blood glucose, and body temperature were checked every week and then organs, blood serums were collected after treatment. The oral glucose tolerance test was also carried out after treatment. Results: Compared to HFD, CAPA extract treated mice showed significant decreases in body weight, adipose tissue weight, lipid accumulations in liver and serum lipid levels without a reduction of food intake. And fasting glucose and glucose tolerance were all improved in the CAPA treated mice. Conclusions: Our results suggest that CAPA extract can prevent diet induced obesity and glucose intolerance without a reduction of energy intake in obese mice.

• 생약학회지
• /
• 제51권4호
• /
• pp.244-254
• /
• 2020

Atractylodes macrocephala is a perennial herb and is a member of the Compositae family. This plant is known to contain various bioactive constituents indicating anti-inflammatory, neuroprotective, anti-oxidant, immunological enhancement, and gastroprotective effects. In this investigation, we isolated four compounds with similar chemical structures from A. macrocephala, and evaluated their anti-inflammatory effects. Among the four compounds, compound 2(atractylenolide II) showed the second-best inhibitory effect on the lipopolysaccharide(LPS)-induced production of nitric oxide in RAW264.7 macrophages and BV2 microglial cells. Compound 2 also inhibited the LPS-induced the production of prostaglandin E2(PGE2), and the expression of inducible nitric oxide synthase(iNOS) and cyclooxygenase(COX)-2 proteins in both cells. In addition, compound 2 suppressed the production of pro-inflammatory cytokines including interleukin(IL)-1β, IL-6, and tumor necrosis factor(TNF)-α. These inhibitory effects were contributed by inactivation of nuclear factor kappa B(NF-κB) and mitogen-activated protein kinases(MAPKs) pathways by treatment with compound 2. This compound did not induce the expression of heme oxygenase(HO)-1 protein indicating that the anti-inflammatory effect of compound 2 was independent with HO-1 protein. Taken together, these results suggested that atractylenolide II can be a candidate material to treat inflammatory diseases.

• 한국자원식물학회지
• /
• 제17권2호
• /
• pp.183-188
• /
• 2004

백출의 재배체계확립시험의 일환으로 노지육묘시 점파(5${\times}$5 cm, 5${\times}$10 cm, 10${\times}$10 cm, 10${\times}$15 cm)와 조파(15 cm)등의 파종방법과 종근중의 무게에 의한 생육과 수량에 미치는 영향을 구명하기 위하여 시험 수행한 결과를 요약하면 다음과 같다. 파종방법별 출아기와 초장은 비슷하였으나 엽수와 지상부 생체중은 육묘 재식거리가 넓을수록 증가 하였으며, 지하부의 근경장과 근경직경도 재식거리가 넓을수록 컸으며 주당 생근중도 5${\times}$5cm의 10.4g에 비해 2.9∼8.7g이 더 무거웠으나 10g 당 생근중은 5${\times}$5 cm에서 1,012kg으로 가장 높았다. 10a 당 종근의 생산량은 재식밀도가 좁은 5${\times}$5 cm와 조파(15cm)에서 많았으나 16g 이상 종근의 생산량은 10${\times}$10 cm에서 가장 높았으며 5${\times}$5 cm에 비해 35% 증가 되었다. 종근중이 무거울수록 출아기는 1∼3일 빨라졌으나 출아율은 비슷하였고, 경장, 경수, 화뢰수 및 생체중에서는 종근이 무거울수록 증가하는 것으로 나타났고 병해는 비슷하였다. 지하부의 생육에서는 근경장, 근경직경이 종근중이 무거울수록 증가하였으며 10 a 당 건근경 수량도 5 g이하 134.6kg에 비해서 6g 이상에서 27∼112% 정도로 증수되었다.