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Screening of Cytotoxicity and Antimicrobial Effects of Extracts from Atractylodes macrocephala Koidz  

Choi Eun Young (Department of Beauty and Skin Care, Kimcheon Science College, Kimcheon)
Oh Hyun Ju (Department of Herbal Resources, Professional Graduate School of Oriental Medicine and Korea Institute of Oriental Pharmacy, Wonkwang University)
Park Nang Kyu (Department of Herbal Resources, Professional Graduate School of Oriental Medicine and Korea Institute of Oriental Pharmacy, Wonkwang University)
Chun Hyun Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine and Korea Institute of Oriental Pharmacy, Wonkwang University)
Ahn Jong Woog (Institute of Chemial Research)
Jeon Byung Hun (Department of Pathology, School of Oriental Medicine, Wonkwang University)
Han Du Seok (Department of Oral Anatomy, School of Dentistry, Wonkwang University)
Lee Hyun Ok (Department of Oral Hygiene. Wonkwang Health Science)
Baek Seung Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine and Korea Institute of Oriental Pharmacy, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.16, no.2, 2002 , pp. 348-352 More about this Journal
Abstract
This study was carried out to evaluate cytotoxic effects of Atractylodes macrocephala Koidz. (A. macrocephala Koidz.) extract on NIH 3T3 fibloblast. SK-MEL-3 (HBT 69) and KB (ATCC No, OCL 17) cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay. 10.2 mg/ml Concentration of A. macrocephala Koidz. extracts in SK-MEL-3 showed that their susceptibility (sensitivity) to these compounds decreased in the following order ; adriamycin > H₂O > ethyl acetate > ethyl alcohol > chloroform > n-hexane in SK-MEL-3 cell lines ; 5-FU > H₂O > n-hexane > ethyl acetate > ethyl alcohol > chloroform in KB cell lines. In order to develop an antimicrobial agent, A. macrocephala Koidz. was extracted with solvents. The minimal inhibitory concentrations (MICs) of each solvent extract of A. macrocephala Koidz. against microogranisms were also examined. Antimicrobial activities of ampicillin and ketoconazole as references were compared to those of each solvent extract of A. macrocephala Koidz. The antimicrobial activity of the ethyl acetate soluble extract of A. macrocephala Koidz. had growth inhibition activity against S. mutans and P. putida (MICs. 500 ㎍/ml). These results suggest that the ethyl acetate soluble extract of A. macrocephala Koidz. possessed antitumorous and antimicrobial agents
Keywords
A. macrocephala Koidz.; MTT assay; Cytotoxic effects; Antimicrobial activities;
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