• 생약학회지
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• 제20권2호
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• pp.88-95
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• 1989

Traditional Chinese herbal drugs, namely, Atractylodes Rhizome and Atractylodes Rhizome White were identified by means of chemical analysis such as TLC and GC/MS. In Korea, the old-grown and the newly-grown rhizomes of Atractylodes japonica Koidzumi (Compositae) are used as the Atractylodes Rhizome and the Atractylodes Rhizome White, respectively. Both rhizomes contain the atractylon asa major spot on TLC. The content of atractylon in a newly-grown rhizome of A. japonica (Atractylodes Rhizome White called in Korea) appears to contain much larger quantity in comparison with that of an old-growon one (Atractylodes Rhizome). The TLC spot pattern analysis showed that rhizomes of A. japonica and A. macrocephala contain the atractylon as a major indicator spot, but no atractylodin (extreamly small amount). However, rhizomes of A. lancea De Candolle and A. koreana Kitamura contain the atractylodin as a major indicator spot.

• 생약학회지
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• 제20권2호
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• pp.96-100
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• 1989

Traditional herbal drugs of Korea, namely, Atractylodes Rhizome(蒼朮) and Atractylodes Rhizome White(白朮) which are derived from and old-grown rhizome and a newly-grown rhizome of Atractylodes japonica Koidz.(Compositae), respectively, were investigated their potential liver-protective activities against hepatotoxicity induced by $CCl_4$ intoxication in mice. Each herbal drug was extracted separately with chloroform, methanol and butanol. No extract of Atractylodes Rhizome showed a significant reduction of the duration of hypnosis produced by hexobarital after $CCl_4$ intoxication in mice. In addition, the the treatments of Atractylodes Rhizome extracts produced no decrease of aspartate aminotransferase(EC 2.6.1.1) activity in serum. However, the methanol extract of Atractylodes Rhizome White exhibited a marked protection from hepatotoxicity induced by $CCl_4$ intoxication in mice. It produced significant reductions of the duration of hypnosis and serum enzyme activity, but no other extract showed liver-protective activity against $CCl_4-induced$ hepatotoxicity.

• 약학회지
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• 제43권6호
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• pp.729-735
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• 1999

Scopolia rhizome is mistaken as an atractylodes rhizome because of their similarities in shape. That is why atractylodes rhizome imported from China sometimes contain scopolia rhizome, which is very toxic. 8 persons were intoxicated atractylodes after taking imported atractylodes rhizome which is tainted. In kampo medicine prepared with such imported atractylodes rhizome, the level of tropane alkaloids ranged from 1.12∼4.34 mg/dose. In this study, we tried to investigate the tissue distribution of scopolia rhizome in rats. The extracts of scopolia was administered orally to rats (a single dose of 10mg/kg, 20mg/kg and 7 days repeated dose of 10mg/kg). Their blood was collected at 0.5, 1, 2, 4, 6 hrs, and liver, kidney, lung and spleen were collected after 6 hrs. The tissue homogenate was applied to solid phase extraction column for the determination of tropane alkaloids. After the oral administration of 20mg/kg scopolia extracts, l-hyoscyamine was detected in rat blood to 2 hrs after dosing. The concentration of tropane alkaloids was the highest in liver followed by lung, kidney and spleen. However, lung, kidney and spleen were similar in amount.

• Preventive Nutrition and Food Science
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• 제15권2호
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• pp.147-151
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• 2010

The volatile aroma constituents of Atractylodes japonica rhizome were separated by steam distillation extraction method using a Clevenger-type apparatus, and analyzed by gas chromatography-mass spectrometry (GC/MS). The yield of the essential oil from Atractylodes japonica was 1.0% (v/w), and its color was pale yellow. Forty-five volatile flavor compounds, which make up 93.86% of the total peak area, were tentatively identified in the rhizome oil. The oil contained 32 hydrocarbons (79.19%) with sesquiterpene hydrocarbon predominating, 3 esters (12.46%), 4 alcohols (0.11%), 1 ketone (0.01%), 2 aldehydes (0.02%), and 3 miscellaneous compounds (2.07%).

• 생약학회지
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• 제19권4호
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• pp.228-232
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• 1988

From the rhizome of Atractylodes japonica Koidzumi (Compositae) which is the original plant of oriental medicine 'Cang-Zhu', four essential oil compounds were isolated. Three of them were identified as atractylon, hydroxyatractylon and $5{\alpha}H,\;10{\beta}-selina-4(14),\;7(11)-diene-8-one$, which were already known as the constitutents of Atractylodes Rhizoma. The fourth is a novel polyacetylene type compound, and the structure is postulated as 1, 4-diacetoxytetradeca-6, 12-diene-8, 10-diyne by the spectral data.

• 한국약용작물학회지
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• 제27권6호
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• pp.404-411
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• 2019

Background: In September 2017, wilting and rhizome rot symptoms were observed on Atractylodes macrocephala Koidz. in Jecheon-si and Eumseong-gun. This study was carried out to isolate hitherto unidentified pathogenic fungi from A. macrocephala and to test the pathogenicity of isolated fungi against Atractylodes spp. genus such as A. macrocephala, A. japonica, and their interspecific hybrids. Methods and Results: The diseased plants were washed with running tap water, and the boundary between the healthy area and the diseased area was cut while the pathogens were isolated by growing cultures from the diseased areas on Phytophthora semi-selective medium. The internal transcribed spacer (ITS) region of the isolates was used in this study for identification. Test plants were cultivated in the glasshouse at 20℃ - 30℃ for 4 months and then used for pathogenicity test. The pots with plants inoculated with mycelial plugs and zoospores were placed at 25℃ for 48 h in a dew chamber where relative humidity was above 95%, and then moved into the glasshouse at 20℃ - 30℃. The presence or absence of pathogenicity of the strains was determined by evaluating the symptom of plant wilting. The inoculation test was performed in three replicates with a non-treated control. Conclusions: On the basis of results of ITS sequence analysis, the strains isolated from the diseased plants was identified as Phytophthora sansomeana. Biological assay using test plants confirmed the pathogenicity of P. sansomeana against Atractylodes macrocephala. This is the first report of rhizome rot in A. macrocephala caused by P. sansomeana.

• 한국자원식물학회지
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• 제13권2호
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• pp.118-123
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• 2000

경북 북부지역에서 큰삽주의 1년생 종근의 3요소 적정 시용량을 구명하고자 1997∼1998년간 수행한 결과를 요약하면 다음과 같다. 1. 3요소 시용량에 따른 지상부의 생육은 N과 K는 수준이 증가할수록 감소하였고 P는 증가하였다. 2. N-P-K=8-9-3kg/10a 시용시 지상부 건물중이 가장 많았고, 지하부는 N-P-K=8-6-3 kg/10a 시용시 10a당 수량이 가장 높았다. 3. 3요소 적정 시용량을 추정한 결과 N은 6, P는 4, K는 6kg/10a로 판단되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.162-162
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• 2017

Atractylodes japonica and Atractylodes macrocephala belongs to Atractylodes genus and theirs roots are used as 'Baek-chul'(White atractylodes rhizome) in Korea Pharmacopoeia. 'Baek-chul' widely used in traditional herbal remedies in Asia. Concurrently, the 'Baek-chul' demand enormously increased in the market due to the growing interest in favoring health benefits but it is depend on import from China. Thus we conducted breeding research to solve the problems associated with domestically cultivated 'Baek-chul' such as low productivity and low disease tolence. We crossed A. japonica (female parent) and A. macrocephala (male parent) in the greenhouse in September 2000. the next season 119 crossed line seeds were collected and germinated in the green house. Among them we selected the good lines in the same season. then conducted replicated yield trials (RYT) in 2014 to local adaptability test (LAT) in 2015-2016. The results showed that the 'AJM16' line has beneficial charactersitcs like, increased plant height, large rhizome diameter, narrow leaf width compared with a 'Sang-chul' (check cultivar) and varied flower color from A. japonica (female parent). The line 'AJM16' was also exhibited decreased anthracnose symptoms(Colletotrichum gloeosporides Penz.). Simultaneously the root yield of AJM16 was 2,409 kg per are, which was increased 130% higher yield compared to 'Sang-chul' (check cultivar) at three various regions from 2015 to 2016. Therefore, we named AJM16 as new cultivar 'Man-chul'. According the results, we have developed a new Atractylodes spp. hybrid cultivar 'Macn-chul' with high yielding and disease tolerance by National Institute of Horticulture and Herbal Science, RDA, in 2016.

• 한국자원식물학회지
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• 제14권3호
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• pp.188-195
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• 2001

삽주(Atractylodes japonica Koidz. ex Kitam.)와 큰꽃삽주(A. macrocephala Koida.)의 근경 과 측근, 줄기, 잎과 엽병, 자방, 잎의 표피 세포의 형태와 크기에 관한 해부학적형질을 조사하여 이들의 분류학적 가치를 평가하고자 본 연구를 수행하였다. 줄기와 자방의 횡단면 구조는 매우 유사하여 두 종이 구분되지 않았으나, 근경에 분포하는 유관의 크기와 단위면적 당 분포수, 잎과 엽병의 횡단면 형태, 잎 표피 세포의 크기와 기공의 단위 면적당 분포수에서 차이가 있어 두 종을 구별하는 형질로서 가치가 있는 것으로 생각되었다.

• 한국자원식물학회지
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• 제12권4호
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• pp.276-281
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• 1999

경북 북부지역에서 큰삽주의 1년생 종근 정식적기를 구명하고자 1996년부터 1998년까지 3개년간 수행한 결과는 다음과 같다. 1. 정식시기별 출현일수는 만식할수록 단축되는 경향이었고 정식시기별 지상부 생육은 3월 20일 정식시 초장이 가장 크고, 주당 지상부 생체중 및 건괴근중은 4월 5일 정식이 각각 51.9, 11.5g으로 가장 양호하였다. 2. 10g당 최대 건괴근수량을 보이는 생육일수는 209일이었으며 경북 북부지역의 큰삽주 1년생 종근 정식 적기는 4월 5일부터 4월 10일 사이로 판단되었다. 3. 생육초기 적산온도가 267$^{\circ}C$일 때 출현일수가 14.4일로 가장 짧았으며 전 생육기간 동안의 적산온도는 3$600^{\circ}C$일 때 10a당 건괴근수량이 233kg으로 가장 많았다.