YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Tissue Distribution of Tropane Alkaloids in Rats and its Determination by GC/MS After the Oral Administration of Scopolia Rhizome

GC/MS에 의한 tropane alkaloids의 분석 및 흰쥐의 생체내 분포

  • 임미애 (국립과학수사연구소 법과학부 약독물과) ;
  • 백승경 (국립과학수사연구소 법과학부 약독물과) ;
  • 이주선 (국립과학수사연구소 법과학부 약독물과) ;
  • 박세연 (국립과학수사연구소 법과학부 약독물과)
  • Published : 1999.12.01
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Abstract

Scopolia rhizome is mistaken as an atractylodes rhizome because of their similarities in shape. That is why atractylodes rhizome imported from China sometimes contain scopolia rhizome, which is very toxic. 8 persons were intoxicated atractylodes after taking imported atractylodes rhizome which is tainted. In kampo medicine prepared with such imported atractylodes rhizome, the level of tropane alkaloids ranged from 1.12∼4.34 mg/dose. In this study, we tried to investigate the tissue distribution of scopolia rhizome in rats. The extracts of scopolia was administered orally to rats (a single dose of 10mg/kg, 20mg/kg and 7 days repeated dose of 10mg/kg). Their blood was collected at 0.5, 1, 2, 4, 6 hrs, and liver, kidney, lung and spleen were collected after 6 hrs. The tissue homogenate was applied to solid phase extraction column for the determination of tropane alkaloids. After the oral administration of 20mg/kg scopolia extracts, l-hyoscyamine was detected in rat blood to 2 hrs after dosing. The concentration of tropane alkaloids was the highest in liver followed by lung, kidney and spleen. However, lung, kidney and spleen were similar in amount.

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References

  1. 대한약전 제 7개정
  2. 원색한국약용식물도감 육창수
  3. Handbook of Poisoning(12th ed.) Dreisbach, R. H.;Robertson, W. O.
  4. J. Chromatogr. v.567 Determination of atropine in biological specimens by high performance liquid chromatography Okuda, T.;Nishida, M.;Sameshima, I.
  5. J. Anal. Toxicol. v.15 Scopolamine poisoning from homemade 'Moon Flower' wine Smith, E. A.;Meloan, C. E.;Pickell, J. A.;Oehme, F. W.
  6. Chem. Pham. Bull. v.37 no.9 Application of ion-pair high performance liquid chromatography for analysis of hyoscyamine and scopolamine in Solanaceous crude drugs Oshima, T.;Sagara, K.;Tong, Y. Y.;Zhang, G.;Chen, Y.H.
  7. J. Pharmaceu. Sci. v.71 no.8 Belladonna alkaloids and phenobarbital combination pharmaceuticals analysis I: High performance liquid chromatographic determinations of hyoscyamine-atropine and scopolamine Pennington, L. J.;Schmidt, W. F.
  8. Ther. Drug Monitoring v.14 Comparative absorption of atropine from a metered-dose inhaler and an intramuscular injection Kehe, C. R.;Lasseter, K. C.;Miller, N. C.;Wick, K. A.(etc.)
  9. J. Anal. Toxicol. v.13 Determination of atropine in blood by gas chromatography/mass spectrometry Saady, J. J.;Poklis, A.
  10. J. Ethnopharm. v.49 Alkaloids of Datura ferox from Argentina Vitale, A. A.;Acher, A.;Pomilio, A. B.
  11. J. Pharmaceu. Biomed. Anal. v.14 Development and validation of an LC/MS/MS method for the determination of l-hyoscyamine in human plasma Xu, A.;Havel, J.;Linderholm, K.;Hulse, J.
  12. J. Chromatogr. v.562 Analysis of tropane alkaloids with thermospray high performance liquid chromatography-mass spectrometry Auriola, S.;Martinsen, A.
  13. Acta. Med. Leg. Soc.(Liege). v.39 no.2 Results of toxicological investigations of poisoning with atropine and scopolamine Sticht, G.;Kaferstein, H.;Staak, M.