• 한국식품영양과학회지
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• 제29권4호
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• pp.712-718
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• 2000

정향으로부터 알칼리 추출시 열수 추출보다 3~6 배의 높은 항응고 활성을 기대할 수 있었으며 최적 추출조건은 1.0 N NaOH, 7$0^{\circ}C$임을 알 수 있었다. 정향을 alkali 추출, 에탄올침전, cetablon 처리 및 한외여과를 이용하여 항응고 활성 획분 EC-2B와 EC-2C를 얻었다. 이 두 획분은 열수 추출보다 각각 6.57배 및 8.63배 높은 항응고 활성획분으로 이는 heparin과 비교시 5.0 unit/mg과 EC-2C는 8.8 unit/mg에 해당되는 활성이다. 이 두 획분을 이용하여 수육을 제조하여 관능검사를 실시한 결과 EC-2B 획분은 정향원물과 유사한 기호도를 가지고 있었으나 EC-2C 획분은 약간의 화학취 로 기호도가 낮았다. 보존성 향상효과 또한 정향원물과 정향추출물(EC-2B 및 EC-2C)에서 관찰되었으며 병원성 미생물에 대한 항균 효과측정 결과 두 획분 모두 항균성을 획인할 수 있었다. EC-2B의 경우 황색포도구균은 0.016% 이상부터 EC-2C는 대장균과 황색포도상ㄱ 균에서는 0.004%에서도 생육 저해효과가 나타났다. EC-2C 획분이 항응고 활성과 항균활성 이 우수하나 안전성과 기호도에서 EC-2B가 우수하였다. 따라서 정향으로부터 추출한 두 항 응고 획분 중 EC-2B 획분이 기능성 식품의 소재로 적합함을 알 수 있었다.

• Food Science and Biotechnology
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• 제15권5호
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• pp.763-767
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• 2006

The anticoagulant properties of compounds derived from fennel (Foeniculum vulgare Gaertner) fruits were evaluated using a platelet aggregometer and compared with aspirin. The active constituents of fennel fruits were isolated and identified as (+)-fenchone and extragole by various spectral analysis techniques. With regard to the 50% inhibitory concentration ($IC_{50}$), (+)-fenchone effectively inhibited platelet aggregation induced by treatment with collagen ($IC_{50}$, $3.9\;{\mu}M$) and arachidonic acid (AA) ($IC_{50}$, $27.1\;{\mu}M$), and estragole inhibited collagen-induced platelet aggregation ($IC_{50}$, $4.7\;{\mu}M$). By way of comparison, (+)-fenchone and estragole proved to be significantly more potent than aspirin at inhibiting platelet aggregation induced by collagen. The inhibitory activity of (+)-fenchone toward platelet aggregation induced by AA was 1.3 times stronger than that of aspirin. These results indicate that (+)- fenchone and estragole may be useful as lead compounds for inhibiting platelet aggregation induced by arachidonic acid and collagen.

• 생약학회지
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• 제30권4호
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• pp.409-412
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• 1999

The in vitro anticoagulant and fibrinolytic activities of crude extract from insects which have been used as traditional medicines. The extracts of Formica, Huechys and Eupolyph-aga/Steleophaga prolonged activated partial thromboplastin time and thrombin time compared to the value of the control. The fibrinolytic activity of insect extracts was also tested by fibrin plate method. We found that the extracts of Cicadae Periostracum, Eupolyphaga/Steleophdga, Mantidis $O{\ddot{o}}otheca$ and Huechys directly could hydrolyze the fibrin clot without the activation of plasminogen by plasminogen activators.

• 대한본초학회지
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• 제23권4호
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• pp.191-196
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• 2008

Objectives: This research was investigated to find out the effect of the anticoagulant Perillae folium extract. Methods: To examine an active effect of anticoagulation in Perillae folium extract, the study measured Prothrombin time(PT) and activated partial thromboplastin time(APTT) of human plasma in vitro and measured bleeding time and arterio-venous shunt model in rats in vivo. Results: Bleeding time of Perillae folium extract in vivo had a significant increase 1.6 times and thrombus weight of Perillae folium extract had a significant reduction of thrombus weight as 68%. Perillae folium extract had an effect of anticoagulation by operating on extrinsic pathway factor II, V, VII, X and intrinsic pathway factor VIII, IX, X, VI, VII in the coagulation system. Conclusions: Considering the above mentioned results, it is judged that a Perillae folium extract has a control effect of thrombus creation.

• Journal of Applied Biological Chemistry
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• 제65권2호
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• pp.107-111
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• 2022

Biological activities such as antioxidant, anticoagulant, and α-glucosidase inhibitory effects of 40% (v/v) ethanolic extract from black locust (Robinia pseudoacacia L.) flower were investigated. The polyphenol content of the black locust flower extract was 39.8±0.5 mg gallic acid equivalents/g. The flower extract represented antioxidant effects such as free radical, cationic radical, and nitrite scavenging abilities as well as reducing power. Also the flower extract inhibited α-glucosidase activity and common pathway in plasma coagulation system.

• Macromolecular Research
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• 제14권1호
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• pp.73-80
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• 2006

Sulfonated poly(ethylene glycol) (PEG-$SO_{3}$) grafted polyrotaxanes (PRx-PEG-$SO_{3}$) were prepared in order to utilize the unique properties of PEG-$SO_{3}$ and the supramolecular structure of PRx, in which PEG-$SO_{3}$ grafted $\alpha$-cyclodextrins ($\alpha$-CDs) were threaded onto PEG segments in a PEG-b-poly(propylene glycol) (PPG)-b-PEG triblock copolymer (Pluronic) chain capped with bulky end groups. Some of the PRx-PEG-$SO_{3}$ demonstrated a higher anticoagulant activity in case of PRx-PEG-$SO_{3}$ (P 105), and compared with the control they showed a lower fibrinogen adsorption in PRx-PEG-$SO_{3}$ (F68) and a higher binding affinity with fibroblast growth factor. The obtained results suggested that polyrotaxane incorporated with PEG-$SO_{3}$ may be applicable to the surface modification of clinically used polymers, especially for blood/cell compatible medical devices.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1993년도 제2회 신약개발 연구발표회 초록집
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• pp.33-33
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• 1993

Among the Glycosaminoglycans (GAGs), Heparin and its fractions or fragments, obtained by several different processes, are of considerable interest .In these last few years, the goal of the researchers in this field has been finding molecular species having selective action, like for instance species having only antithrombotic activity disjointed from any anticoagulant effect, and assessing the effects of these GAGs and of other GAGs, like dermatan sulphate, not only in the field of venous or arterial thrombosis but also on cell factors like smooth muscle cell proliferation and even on aspects of antiinflammatory activity.

• 한국해양바이오학회지
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• 제7권2호
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• pp.71-78
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• 2015

Sulfated polysaccharides from plants or seaweed are well known for antioxidant, anticoagulant activity or other biological activities. The aim of the present study is to optimize the condition for the isolation of water soluble polysaccharides (WSP) and evaluate the biological activity. WSP was isolated from defatted microalgae B. braunii using chlorosulfonic acid or sulfuric acid. Among WSP isolated using sulfuric acid showed the lowest the content of total carbohydrates (g/L), whereas showed the highest antioxidant activity. Also the preliminary structural analysis of WSP was performed by FT-IR spectroscopy analysis.

• Archives of Pharmacal Research
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• 제16권3호
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• pp.209-212
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• 1993

The anti-coagulant activity of ilexoside D isolated from the roots of ilex pubescens Hook. et Am. was investigated in in vivo models of blood coagulation in rats. On oral administration, ilexoside D prolonged the bleeding time and the whole blood recalcified clotting time, but not the plasma recalcified clotting time. In vitor, ilexoside D did not affect the recalciffed clotting times of whole blood, platelet-rich plasma(PRP), and platelet-poor plasma(PPP), while in the presence of tissue factor the compound prologed the reduced proth-rombin times of whole blood, PRP and PPP in the dose-dependent manner. These results indicate that ilexoside D has the anit-tissue factor activity as well as the antithromobotic activity.

• The Korean Journal of Physiology and Pharmacology
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• 제12권5호
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• pp.231-236
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• 2008

Heparin is a well-known anticoagulant widely used in various clinical settings. Interestingly, recent studies have indicated that heparin also has anti-inflammatory effects on neuroinflammation-related diseases, such as Alzheimer's disease and meningitis. However, the underlying mechanism of its actions remains unclear. In the present study, we examined the anti-inflammatory mechanism of heparin in cultured cerebral endothelial cells (CECs), and found that heparin inhibited the tumor necrosis factor $\alpha$ ($TNF{\alpha}$)-induced and nuclear factor kappa B (NF-${\kappa}B$)-dependent expression of adhesion molecules, such as intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1), which are crucial for inflammatory responses. Heparin selectively interfered with NF-${\kappa}B$ DNA-binding activity in the nucleus, which is stimulated by $TNF{\alpha}$. In addition, non-anticoagulant 2,3-O desulfated heparin (ODS) prevented NF-${\kappa}B$ activation by $TNF{\alpha}$, suggesting that the anti-inflammatory mechanism of heparin action in CECs lies in heparin's ability to inhibit the expression of cell adhesion molecules, as opposed to its anticoagulant actions.