• 대한본초학회지
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• 제32권2호
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• pp.49-56
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• 2017

Objectives : This study investigated the anti-oxidant activities and improving effect of Phellinus linteus and Glycyrrhiza uralensis Extract (PGE) on Atopic Dermatitis. Methods : 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical, Hydrogen peroxides scavenging activities and Superoxide dismutase (SOD)-like activities were used for the measurement of anti-oxidant ability. Cytotoxicity of PGE in Raw 264.7 cell was evaluated by MTT assay. To evaluate the anti-atopic dermatitis effect of PGE, a total of 33 patients with atopic dermatitis were observed trans epidermal water loss, skin moisture content, modified SCORAD index of atopic dermatitis and pruritic degree after applying the PGE for 4 weeks. Results : PGE scavenged DPPH ($IC_{50}=25ppm$) effectively, ABTS and Hydrogenperoxides scavenged similar to BHA. As for the SOD-like activity, it had lower effect than ascorbic acid, but it comparable activities in 500ppm. There was no cytotoxicity at PGE at concentrations of 10,000ppm. In clinical research about PGE on patients with atopic dermatitis, skin condition was improved. After 4 weeks, the application of PGE increased skin moisture content from 19.43 to 31.22. Moreover, it reduced the skin temperature (from 32.5 to 31.9), skin pH (from 5.39 to 5.22), trans epidermal water loss (from 39.03 to 24.46) and pruritus score (from 6.07 to 3.87). In addition, the Modified SCORAD index decreased from 31.28 to 20.3. Conclusions : In conclusion, PGE possesses anti-oxidant and anti-atopic dermatitis activities, thus it could be potentially valuable as anti-atopic dermatitis material.

• 한방안이비인후피부과학회지
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• 제33권1호
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• pp.1-11
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• 2020

Objectives : The purpose of this study was to investigate the anti-inflammatory and anti-oxidative effects of Osung-tang(OST) extract. Methods : MTT assay was performed to confirm the survival rate of RAW 264.7 cells treated with OST extract(50-400㎍/㎖) and the production of NO from LPS-induced RAW 264.7 cells was confirmed. The mRNA expression of iNOS, COX-2, and NF-κB were measured by real-time PCR. The protein expression of iNOS, COX-2 and p-IκB were measured by western blot and the anti-oxidant activity of OST extract(50-400㎍/㎖) was investigated by measuring DPPH scavenging activity. Results : OST extract showed a cell survival rate of 90% or more at 50-400㎍/㎖. The NO production was inhibited in a dose-dependent manner in RAW 264.7 cells treated with LPS on OST treated group. mRNA expression levels of iNOS, COX-2 and NF-κB decreased in a concentration-dependent manner after treatment with OST(50-400㎍/㎖). Protein expression levels of iNOS, COX-2 and p-IκB decreased in a concentration-dependent manner after treatment with OST(50-400㎍/㎖). It was found that OST has a high free-radical scavenging ability. Conclusions : These results suggest that OST extract can be used as a treatment for various skin diseases by demonstrating its anti-inflammatory and anti-oxidant effects.

• 대한본초학회지
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• 제38권3호
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• pp.19-26
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• 2023

Objectives : Agrimoniae Herba is a herbal medicine widely distributed in Asia and contains flavonoids including catechin, quercitrin, rutin, hyperoside, and quercetin. This study aimed to investigate the anti-oxidant activity and skin barrier function of different solvent fractions (Hexane; methylene chloride, MC; ethyl acetate, EA; n-butanol, Bu; Water) obtained from Agrimoniae Herba. Methods : Anti-oxidant activity of different solvent fractions obtained from Agrimoniae Herba was investigated through total polyphenol contents, total flavonoid contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity measurements. Then, filament aggregating protein (Filaggrin), Type I collagen, ceramide synthase (CERS) 3, and CERS4 were analyzed to evaluate the skin barrier strengthening effect of different solvent fractions obtained from Agrimoniae Herba on UVB-stimulated HaCaT cells. Results : As a result of measuring total polyphenol contents, total flavonoid contents, DPPH free radical scavenging activity, and ABTS radical scavenging activity, antioxidant activity was found to be excellent in the order of EA > Bu > MC > Hexane > Water. As a result of measuring mRNA gene expression of Type I collagen, Filaggrin, CERS3, and CERS4 after UVB-stimulated was applied to HaCaT cells treated with different solvent fractions obtained from Agrimoniae Herba, it was found to increase significantly in the Bu-treated group. Conclusion : Our findings show that the Bu sample obtained from Agrimoniae Herba has excellent anti-oxidant ability, which increases Type I collagen, Filaggrin, and ceramide synthetase in UVB-stimulated HaCaT cells to control the skin barrier improvement effect.

• 한방안이비인후피부과학회지
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• 제31권4호
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• pp.1-12
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• 2018

Objectives : This study investigated the anti-oxidant and anti-inflammatory activities of Changchulgeumryeontang (CCGRT) extract. Methods : The macrophage cell line RAW 264.7 cells were used and MTT assay was performed to measure the cell viabilities at the various concentrations of CCGRT ($25-200{\mu}g/m{\ell}$). Nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) were measured in LPS-induced RAW 264.7 cells. Expressions of iNOS, $NF-{\kappa}B$, $IL-1{\alpha}$, $IL-1{\beta}$ and IL-6 were also performed by real-time PCR. The anti-oxidant activities of CCGRT was measured by DPPH radical scavenging activity. Results : 1. there was no cytotoxicity in RAW264.7 cells treated with CCGRT compared to the control. 2. CCGRT treated group significantly inhibited NO and $PGE_2$ production compared to the LPS treated group. 3. CCGRT treated group significantly decreased mRNA expressions of iNOS, $NF-{\kappa}B$, $IL-1{\alpha}$, $IL-1{\beta}$ and IL-6 compared to the LPS treated group. 4. CCGTR was found to have high DPPH free radical scavenging ability. Conclusions : According to the above results, CCGRT may be a potentional choice for the treatment of inflammatory skin disease. Conclusions : According to the above results, CCGRT may be a potentional choice for the treatment of inflammatory skin disease.

• 한방안이비인후피부과학회지
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• 제21권3호
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• pp.104-110
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• 2008

Objectives : This experiment study was performed to investigate the anti-inflammation and anti-oxidant effects of Indigo Naturalis(IN) and Rehmanniae Radix(RR) which were herbs for clearing heat. Methods : Anti-inflammation and anti-oxidation effects of IN and RR that were measured by the inhibitory ability of Nitric oxide(NO) production and the scavenging for 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical. Results : 1. IN and RR groups were not showed cell toxicity. 2. In the inhibitory ability of NO production, IN groups were better than RR groups, but there were no statistical significances among the groups. 3. In the scavenging for DPPH radical, IN groups were better than RR groups, but there were no statistical significances among the groups. Conclusions : These results showed that the IN group was better than the RR group in treating skin eruptions, especially those such as erythema, purple spot, papule, pustules which appear in acute inflammation.

• 대한본초학회지
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• 제22권3호
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• pp.85-91
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• 2007

Objective : In this study, anti-oxidant and cosmeceutical activities of Isatis tinctoria L. extracted from water, ethanol and supercritical fluid condition were confirmed to investigate cosmeceutical activities for utilization as cosmetic ingredient. Methods: Anti-oxidant and cosmeceutical activities were investigated by using electron donating ability, xanthine oxidase, tyrosinase, astringent effect. Result : Isatis tinctoria L. extracts by supercritical fluid, water and ethanol showed good electron donating ability which were 82.7%, 62.6% and 44.8% at the concentration at 1,000ppm, respectively. Xanthine oxidase activity related with purine metabolism was inhibited by ethanol extract about 52.3% at the concentration at 1,000ppm. Tyrosinase inhibition effects, by supercritical fluid extract, ethanol extract and water extract, were 83.3%, 52.9% and 41.2% respectively at 1,000ppm. In the measurement of astringent effect, supercritical fluid extract at the concentration to 5,000ppm showed 85.7% in related activity. The water extract showed 95.9% nitrite scavenging activity at 5,000ppm. Conclusion : According to these results, it is possible that the extract of Isatis tinctoria L. can be used as a new natural material of cosmetic industry.

• 생명과학회지
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• 제18권4호
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• pp.509-514
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• 2008

동 ${\cdot}$ 식물의 추출물은 예로부터 생약으로 많이 이용이 되었는데, 이는 추출물 내에 다양한 생리 활성 물질 및 화합물을 포함 하고 있기 때문이다. 본 연구는 한방에서 누고(樓姑)라고 알려진 땅강아지 추출물에 대한 항산화 활성을 1,1-diphenyl-2-picrylhydrazyl (DPPH)와 ferric reducing ability of plasma (FRAP)활성 실험을 통하여 알아보았다. 땅강아지는 DW, DMSO, 에탄올, 메탄올에 추출하였다. 실험 결과 땅강아지 추출물의 항산화활성은 DPPH radical 소거 활성의 경우 에탄올 추출물에서 가장 높은 활성을 나타났다. FRAP 활성의 경우 DW 추출물에서 가장 높게 나타났으며, 시간에 따른 활성의 증가는 에탄올 추출물에서 가장 높게 나타났다. Cyclooxygenase-2 promoter의 활성 저해는 DMSO와 에탄올 추출물에서 저해활성이 상대적으로 높은 것으로 미루어 보아서 이들 추출물간의 특성을 잘 응용할 경우 항산화 및 항염증에 좋은 생물학적 재료가 될 것으로 판단된다.

• Journal of Applied Biological Chemistry
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• 제53권3호
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• pp.162-169
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• 2010

도화 추출물의 항산화능 및 항염증 효과를 검증하여 기능성 화장품 소재로서의 가능성을 검증하였다. 도화의 항산화능 측정으로 전자공여능을 측정한 결과 도화 에탄올 및 아세톤 추출물 500ppm에서 90.0% 이상의 효과를 나타내어, 합성 항산화제로 알려진 BHT와 유사한 효과를 나타내었으며, SOD 유사활성능 측정 결과 도화 에탄올 및 아세톤 추출물 1,000ppm에서 40.0% 이상의 활성을 나타내었다. Xanthine oxidase 저해활성능 측정 결과 도화 에탄올 및 아세톤 추출물 1,000 ppm에서 vitamin C와 유사한 30.0%의 효과를 나타내어, 도화 열수 추출물에 비해 도화 에탄올 및 아세톤 추출물의 효과가 높게 나타났다. 항염증 효과 측정으로 hyaluronidase 저해활성을 측정한 결과 도화 열수, 에탄올 및 아세톤 추출물에서 35.0%의 저해활성을 나타내었으며, macrophage 세포를 이용한 nitric oxide (NO) 저해활성을 측정한 결과 도화 추출물 모든 농도에서 12시간 이후부터 NO 생성량이 억제됨을 확인할 수 있었다. 또한 피부염증 유발균인 Staphylococcus epidermidis와 Propionibacterium acnes의 항균효과를 측정한 결과 도화 에탄올 추출물에서 항균 효과를 나타내었다.

• 대한화장품학회지
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• 제43권2호
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• pp.125-135
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• 2017

본 연구의 목적은 8가지 한약재(가지, 금은화, 감초, 천궁, 당귀, 황련, 치자, 연교)로 구성된 가지청열소 독음의 항산화 효과와 항염증 효과를 검증하여 효과적인 화장품소재로서의 가능성을 확인하는 것이다. 한약재의 구성과 비율은 동의보감에 수록된 청열소독음(淸熱蘇毒飮) 처방을 변형시켜 사용하였고 추출은 열수와 70% 에탄올로 하여 동결건조 분말화하였다. 항산화 효능을 확인하기 위해 라디칼 소거능력(DPPH, $ABTS^+$, superoxide), superoxide dismutase (SOD)유사활성능력, 총 폴리페놀 화합물 함량을 조사하였고 항염증 효능을 확인하기 위해 LPS (lipopolysaccharide)로 염증반응을 유도시킨 RAW264.7 대식세포에서의 nitric oxide(NO)생성 저해력과 western blot 분석을 통한 염증 관련 단백질 inducible nitric oxide synthase (iNOS)와 cyclooxygenase (COX-2)의 발현 저해를 확인하였다. 그 결과, 가지청열소독음은 뛰어난 항산화 효과와 항염증 효과를 나타내었고 화장품을 위한 효과적인 성분으로 사용 가능할 것으로 사료된다.

• 대한한의학회지
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• 제25권2호
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• pp.194-206
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• 2004

Objectives : JiaoTeng-Yuan(교등원) is said to be a prescription for preservation of health in $\ulcorner$HuaTuo.ZhongZangJing (화타.중장경)$\lrcorner$. It is known to have the effect of Bu-Shen (보신: strengthening kidney) and Yi-Shou (익수: prolonging the span of one's life). This study investigates whether JTY is effective on inhibition of oxidation stress. Methods : Sprague-Dawley rats (12-week-old, weight $300{\pm}20g$) were divided into 3 groups. The normal group (n=8) was injected with PBS (1ml/body, s.c) subcutaneously behind the neck. The control group (n=8) was injected with D-galactose (50mg/kg, 1ml PBS/body, s.c) to induce pathological animals. The JTY group was injected with the same treatment as the control group, and fed containing JTY (10%). All groups were treated 1 time per day for 6 weeks. After the rats were sacrificed anti-oxidant enzyme (SOD, CAT, G-px) activity, GSH quantity of RBC and tissue (heart, liver and kidney), and plasma Vit-C quantity were examined. Also, the MDA levels of liver and kidney, lipofuscin of heart and endurance of erythrocyte membrane were measured. Results : In the JTY group, RBC's SOD activity decline was halted by 21 % of the normal level, compared to the control group, G-px activity (unit/g of Hb) increased significantly, compared to the normal group, and the level of Vit-C in plasma increased by 16%. Heart's SOD activity was kept at the same level as that of the normal group; and CAT activity decline was halted by 26%. Kidney's CAT and G-px activities were kept at the same level as that shown in the normal group, implying the existence of a halting effect. Liver also showed a slight halting effect against the decline of anti-oxidant ability, but the effect was not significant (${\alpha}=0.05$). A comparison between the levels of peroxide in SD rats showed that the level of TBARS in plasma increased significantly in the control group and that it was normal in the JTY group. The livers in the JTY group, compared to those in the control group, showed 36% halting effect of the normal level while their kidney's indicated the level significantly lower than the normal level. Heart's lipofuscin increased significantly in the control group, but was alike in both the JTY and the normal groups. Endurance of erythrocyte membrane (%) decreased significantly in the control group while it was kept at the similar level in both the JTY and the normal groups, indicating the halting effect. Conclusions : This study suggests that JTY is effective to defend oxidation stress caused by D-galactose in the animals. It showed that the anti-oxidant ability was maintained and strengthened. On the other hand, it reduced the level of peroxide in animals. In sum, JTY appeared to have the equilibrium normal physiological function in SD rat.