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http://dx.doi.org/10.6114/jkood.2020.33.1.001

Anti-inflammatory and Anti-oxidant Studies of Osung-tang Extracts in LPS-Induced RAW 264.7 Cells  

Kim, So-Jeong (Dept. Of Cosmetic Sciences & Beauty Biotechnology, Semyung University)
kim, Tae-Jun (Dept. Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University)
Kim, Ee-Hwa (Dept. Of Acupoint & Meridian, College of Korean Medicine, Semyung University)
Kim, Yong-Min (Dept. Of Cosmetic Sciences & Beauty Biotechnology, Semyung University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.33, no.1, 2020 , pp. 1-11 More about this Journal
Abstract
Objectives : The purpose of this study was to investigate the anti-inflammatory and anti-oxidative effects of Osung-tang(OST) extract. Methods : MTT assay was performed to confirm the survival rate of RAW 264.7 cells treated with OST extract(50-400㎍/㎖) and the production of NO from LPS-induced RAW 264.7 cells was confirmed. The mRNA expression of iNOS, COX-2, and NF-κB were measured by real-time PCR. The protein expression of iNOS, COX-2 and p-IκB were measured by western blot and the anti-oxidant activity of OST extract(50-400㎍/㎖) was investigated by measuring DPPH scavenging activity. Results : OST extract showed a cell survival rate of 90% or more at 50-400㎍/㎖. The NO production was inhibited in a dose-dependent manner in RAW 264.7 cells treated with LPS on OST treated group. mRNA expression levels of iNOS, COX-2 and NF-κB decreased in a concentration-dependent manner after treatment with OST(50-400㎍/㎖). Protein expression levels of iNOS, COX-2 and p-IκB decreased in a concentration-dependent manner after treatment with OST(50-400㎍/㎖). It was found that OST has a high free-radical scavenging ability. Conclusions : These results suggest that OST extract can be used as a treatment for various skin diseases by demonstrating its anti-inflammatory and anti-oxidant effects.
Keywords
Anti-inflammation; Anti-oxidant; COX-2; iNOS; NF-${\kappa}B$; Osung-tang(OST);
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Times Cited By KSCI : 5  (Citation Analysis)
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