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http://dx.doi.org/10.6114/jkood.2018.31.4.001

The anti-oxidant and anti-inflammatory Activites of Changchulgeumryeontang Extract  

Won, Hea-Ryeon (Dept. of Oriental Cosmetic Sciences, Semyung University)
Park, Hye-Su (Dept. of Oriental Ophthalmology, Otolaryngology & Dermatology, College of Korean Medicine, Semyung University)
Kim, Ee-Hwa (Dept. of Meridian and Acupoint, College of Korean Medicine, Semyung University)
Kim, Yong-Min (Dept. of Oriental Cosmetic Sciences, Semyung University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.31, no.4, 2018 , pp. 1-12 More about this Journal
Abstract
Objectives : This study investigated the anti-oxidant and anti-inflammatory activities of Changchulgeumryeontang (CCGRT) extract. Methods : The macrophage cell line RAW 264.7 cells were used and MTT assay was performed to measure the cell viabilities at the various concentrations of CCGRT ($25-200{\mu}g/m{\ell}$). Nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) were measured in LPS-induced RAW 264.7 cells. Expressions of iNOS, $NF-{\kappa}B$, $IL-1{\alpha}$, $IL-1{\beta}$ and IL-6 were also performed by real-time PCR. The anti-oxidant activities of CCGRT was measured by DPPH radical scavenging activity. Results : 1. there was no cytotoxicity in RAW264.7 cells treated with CCGRT compared to the control. 2. CCGRT treated group significantly inhibited NO and $PGE_2$ production compared to the LPS treated group. 3. CCGRT treated group significantly decreased mRNA expressions of iNOS, $NF-{\kappa}B$, $IL-1{\alpha}$, $IL-1{\beta}$ and IL-6 compared to the LPS treated group. 4. CCGTR was found to have high DPPH free radical scavenging ability. Conclusions : According to the above results, CCGRT may be a potentional choice for the treatment of inflammatory skin disease. Conclusions : According to the above results, CCGRT may be a potentional choice for the treatment of inflammatory skin disease.
Keywords
Anti-inflammation; Anti-oxidant; nitric oxide (NO); Prostaglandin $E_2$ ($PGE_2$); Changchulgeumreontang (CCGRT);
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1 Yoon JM, Kim DI, Lee JH, Han SJ, Kim HE, Kim HJ, et al. Study on Anti-oxidant and Anti-inflammatory Activity of Eggplantcheongyeolsodokum. J Soc Cosmet Sci Korea. 2017;43(2):125-35.   DOI
2 Jeong SI, Kim HS, Jeon IH, Kang HJ, Mok JY, Cheon CJ, et al. Antioxidant and Anti-inflammatory Effects of Ethanol Extracts from Perilla frutescens. KOREAN J FOOD SCI TECHNOL Korea. 2014;46(1):87-93.   DOI
3 Oh HK. Antioxidant and Anti-inflammatory Activities of Extracts from Eugenia caryophyllata Thunb. J Korean Soc Food Cult Korea. 2016;31(5):481-8.   DOI
4 Yi MR, Kang CH, Bu HJ. Antioxidant and anti-inflammatory Activity of extracts from kohlrabi(Brassica Oleracea var. Gonglodes). J of Korean Oil Chemists' Soc Korea. 2017; 34(2):189-202.
5 Yi MR, Jeon AL, Kang CH, Bu HJ. Antioxidant, Antimicrobial and Antiinflammatory Activities of Essential Oil from Erigeron annuus L. Flower. J of Korean Oil Chemists' Soc Korea. 2016; 33(4):717-25.   DOI
6 Kim MK, Kim DY. Anti-inflammatory effect of barley leaf ethanol extract in LPS-stimulated RAW264.7 macrophage. Korean J Food Preserv. 2015;22(5):735-43.   DOI
7 Lee HN, Kim JK, Kwon GT, Shim JH, Kim JD, Park HY. Anti-inflammatory effect of ethanol extract from bark of acer barbinerve Maxim. J Korean Soc Food Sci Nutr. 2012;49(9):1242-7.
8 Ghosh S, Hayden HS. New requlators of NF-${\kappa}B$ in inflammation. Nat Rev Immunol. 2008;8:837-48.   DOI
9 Jeong DH, Kang BY, Kim KBWR, Kim MJ, Ahn DH. Anti-inflammatory activity of Sargassum micracanthum water extract. J Korean Soc Food Sci Nutr. 2014;57(3):227-34.
10 Won SJ, Park HJ, Lee KT. Inhibition of LPS induced iNOS, COX-2 and cytokines expression by salidroside through the NF-${\kappa}B$ inactivation in RAW 264.7 cells. Korean J Pharmacogn. 2008;30(12):110-7.
11 Kim SH, Choi JH, Oh HT, Chung MJ, Cui CB, Ham SS. Cytoprotective effect of antioxidant activity of Codonopsis lanceolata and Platycodon grandiflorum ethyl acetate fraction in human HepG2 cells. Korean J Food Sci Technol. 2008;40(6):696-701.
12 Lee KH, Yoon WH. Effect of protein-bond polysaccharide isolated from Acanthopanax senticosus in reducing the toxic effect of cisplatin. Korean J Pharmacogn. 2007;38(2):1-17.
13 Choi WS, Kwon HS, NO RH, Choi GP, Lee HY. Enhancement of anti-inflammatory activities of fermented Scutellaria baicalensis extracts using Lactobacillus rhamnosus. J Soc Cosmet Sci Korea. 2013;39(4):303-11.   DOI
14 Kim DH, Park SR, Debnath T, Hasnat A, Pervin M, Percin M, et al. Evaluation of the antioxidant activity and anti-inflammatory effect of Hericium erinaceus water extract. Kor J Med Crop Sci. 2013;21(2):112-7.   DOI
15 Jangro. Jangssiuitong. Iljungsa. Seoul, p.495, 1992.
16 Fridovich I. Superoxide dismutases: An adaptation to a paramagnetic gas. J Biol Chem. 1989;264(14):7761-4.
17 Wakamatsu TH, Dogru M, Ayako I, Takano Y, Matsumoto Y, Ibrahim OM, et al. Evaluation of lipid oxidative stress status and inflammation in atopic ocular surface disease. Mol Vis. 2010;16:2465-75.
18 Fang YZ, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutr. 2002;18: 872-9.   DOI
19 Kastle M, Grune T. Protein oxidative modification in the aging organism and the role of the ubiquitin proteasomal system. Curr Pharm Des. 2011;17:4007-22.   DOI
20 Shon MS, Song JH, Kim JS, Jang HD, Kim GN. Anti-oxidant activity of oil extracted from Korean Red Ginseng and its moisturizing function. Kor J Aesthet Cosmetol. 2013;11(3):489-94.
21 Boden G. 45Obesity, insulin resistance and free fatty acids. Curr Opin Endocrinol Diabetes Obes. 2011;18(2):139-43.   DOI
22 Park HR, Kim KY. Inhibition effect of TNF-$\alpha$, IL-$1{\alpha}$ production and TNF-$\alpha$, IL-1 $\alpha$ mRNA expression from chenopodium album ethanol extract. J Korean Soc Cosmetol. 2017;23(5):971-7.
23 Park MH, Kim MR. Analysis of Antioxidant Activity and Cytotoxicity against Human Cancer Cell Lines of Extracts from Atractylodes rhizoma fermented with Ganoderma lucidum Mycelium. Korean J Food Nutr Korea. 2017;30(3):454-63.
24 Yoon SB, Han HS, Lee YJ. Effect of Scutellariae Radix extract on the proinflammatory mediators in RAW 264.7 cells induced by LPS. Kor J Herbology. 2011;26(2):75-81.   DOI
25 Lee JW, Han HS, Lee YJ. Anti-inflammatory Effects of Coptidis Rhizoma Extract. Kor J Herbology Korea. 2014;29(5):83-90.
26 Yang HO, Oh HJ, Park NK, Choi EY, Lee HO, Yang EY, et al. Cytotoxity and Antimicrobial Effects of the Extract of Poncirus trifoliata. Kor J Oriental Preventive Medical Society. 2000;4(2):235-41.
27 Lee YJ, Lee MJ, Park JW, Kim JK, Choi DY, Kim CH. Antioxidant Activity of Water-Extrat from Coptis chinensis Franch. Korean Journal of Life Science. Korea. 2000;10(3):241-6.
28 Song JW, Min KJ, Cha CG. Antioxidative and Antitumor Activity of Extracts from Saussurea lappa. J Env Hlth Sci. 2008; 34(1):55-61.
29 Kim SY, Lee EB, Jeong EJ. Anti-inflammatory action of the fractions of platycodi radix. Kor J Food Nutr. 2009;22(4):618-24.
30 Bak JP, Son JH, Kim YM, Lee EY, Leem KH, Kim EH. Suppression of inflammatory macrophage response Glycyrhiza Uralensis herbal acupuncture extract. Kor J Acupuncture. 2011;28(4):49-58.
31 Ryu JY, Cho HK, Yoo HR, Seol IC, Kim YS. The Effects of Artemisiae Iwayomogii Herba, Curcumae Radix, and Aurantii Fructus Immaturus Complex Extract (ACA) on Dyslipidemia-related Factor Expression and Anti-oxidation in HepG2 Cells. J Int Korean Med. 2017;38(3):367-75.   DOI
32 Song JJ, Park YC. Effects of Pinelliae Rhizoma on immunocyte and cytokine production in asthma model mouse. Korean J Orient Int Med. 2005;26(1):156-68.
33 Cho SW, Kim YK. Studies on Protective Effect of Bupleurum falcatum Extract (SHI-1909) against Experimental Inflammtory Bowel Disease Model. Journal of the Korea Academia-Industrial cooperation Society. 2009;10(3):613-9.   DOI
34 Jung HS, Kang KH, Choi YH, Choi BT, Lee YT. Anti Allergic Effects of Cimicifuga Racemosa on Allergic Models. Korean J Oriental Physiology & Pathology. 2006;20(2):404-9.
35 Shon MY. Antioxidant and Anticancer Activities of Poria cocos and Machilus thunbergii Fermented with Mycelial Mushrooms. Food Industry and Nutrition. 2007;12(2):51-7.
36 Lee JY, Yoo DH, Joo DH, Kim SR, Jo HS, Joo SH, et al. Anti-inflammatory effects of amelanchier asiatica fruits ethanol extract. J Soc Cosmet Sci Korea. 2017;43(1):19-26.   DOI
37 Kim JG, Kang YM, Eum GS, Ko YM, Kim TY. Antioxidative Activity and Antimicrobial Activity of Extracts from medicinal plants(Akebia quinate Decaisn, Scirusfluviatilis A. Gray, Gardenia jasminoides for. grandiflora Makino). J Agriculture & Life Sciences. 2003;37(4):69-75.
38 Yong SE, Park PS, Lim JM, Kwon HJ, Choi JH, Choi YH, et al. Studies on Antioxidant and Antidiabetic Effects of Fermented Cnidium officinale Makino. Kor J Herbology. 2011;26(4):109-13.   DOI