• Journal of Plant Biotechnology
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• 제43권2호
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• pp.157-163
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• 2016

본 연구는 국내 동, 서 및 남해안 바닷가 근처 사구지역에서 자생하는 번행초 55개체를 수집하여 AFLP 마커시스템을 적용하고 이들 유전자원들간의 유전다양성 차이를 알아보기 위하여 실시하였다. 우선 전체 게놈의 특이적 부위를 절단하기 위하여 제한효소로 EcoRI과 MseI 12개 조합을 활용하였고, 그 결과 총 1,279 절편을 확보할 수 있었다. 이 결과는 제한효소 조합당 평균 107개의 절편이 생산된 것으로 이 중 평균 62개(약 58%)가 유전자원간에 다형성을 나타냈다. 이와 같이 유전자원간에 다형성을 보인 게놈 절편을 대상으로 유전다양성을 분석한 결과 조사된 55개체 번행초 유전자원 집단은 29%의 유전적 차이를 보이는 것을 알 수 있었다. 또한 군집분석을 통해 유전적 차이를 보이는 그룹을 분류한 결과 국내 자생 번행초 유전자원은 총 7개의 집단으로 나누어짐을 알 수 있었다. 본 연구에서 국내외 최초의 번행초 유전 다양성 평가 정보는 향 후 품종 육성을 위한 교배친의 선발에 적용하여 다양한 유전적 차이를 보이는 분리집단을 확보하는 데 활용이 가능할 것으로 생각된다.

• 방사선산업학회지
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• 제5권4호
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• pp.347-352
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• 2011

In order to identify the genetic relationship analysis by acute and chronic gamma irradiation, Arabidopsis (Arabidopsis thaliana L.) were irradiated with 200 Gy of gamma-rays using gamma-irradiator (3,000 Ci; Nordion, Canada) and gamma-phytotron (400 Ci; Nordion, Canada) for acute and chronic irradiation, respectively. Genetic relationship among two acute gamma-irradiated plants (A1 and A24) and three chronic gamma-irradiated plants (C1W, C2W, C3W) were analyzed using the amplified fragment length polymorphism (AFLP) technique compared with each non-irradiated plant. A total of 28 EcoRI and MseI primer combinations were used to screen 8 treatments by the ABI3130 capillary electrophoresis system. Amplified products by 28 primer sets showed 1,679 bands with an average of 51 bands per primer combination. Out of the total bands scored, 1,164 fragments were polymorphic bands, with different alleles existing among the treatments. The cluster analysis was performed using the UPGMA (Unweighted Pair Group Method using Arithmetic) in the computer program NTSYS-pc. In clustery analysis, acute gamma-irradiation showed higher genetic variation compared with chronic gamma-irradiation.

• 한국육종학회지
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• 제41권4호
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• pp.444-450
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• 2009

본 연구는 배 유전자원의 유전적 변이를 DNA 수준에서 비교함으로써 육종의 기초 자료로 활용하기 위하여 유전자원 60점을 대상으로 AFLP 분석을 수행하였다. 총 20종의 AFLP 프라이머 조합을 이용하여 522개의 다형성 밴드를 얻었다. 획득된 다형성 밴드를 이용하여 UPGMA 방식으로 유사도 및 집괴분석을 수행한 결과 유전적 유사도 0.691를 기준으로 4개의 그룹으로 분류되었다. 첫 번째 그룹에는 Pyrus communis에 속하는 품종 및 P. nivalis, P. cordata 등이 포함되었다. P. betulaefolia와 P. fauriei에 속하는 콩배 계통들이 두 번째 그룹에 속하였고, P. calleryana와 P. koehnei를 포함한 콩배 계통들이 세 번째 그룹으로 분류되었다. 네 번째 그룹에는 P. pyrifolia와 P. ussuriensis에 속하는 재배품종, 교잡종 및 그 외의 종들이 대부분 속하여 동아시아에서 유래한 유전자원들은 P. pyrifolia나 P. ussuriensis와 서로 밀접히 연관되어 있음을 확인할 수 있었다. 유전자원 간 유전적 유사도는 0.584에서 0.879범위로 평균 유전적 유사도는 0.686이었다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.123.2-124
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• 2003

Fusarium graminearum (telomorph：Gibberella zeae), an important fungal pathogen of cereal crops with ubiquitous geographic distribution, produces mycotoxins on diseased crops that has threaten human and animal health. Recently severe epidemics of scab diseases of barley and rice by this fungus occurred in Korea, causing serious economic losses. To determine genetic diversity of F. graminearum from rice in Korea, a total of 269 isolates were obtained from Southern part of Korea during 2001-2002. A phylogenetic tree of the isolates was constructed by using amplified fragment length polymorphism (AFLP). Population structure of the rice isolates consists of a single lineage (lineage 6). Frequency of female fertility among these Isolates was relatively low (37％) compared to that among lineage 7 isolates from Korean corn. PCR amplification using chemotype specific primers derived from Tri7 and Tri13 genes at the trichothecene biosynthesis gene cluster revealed that most isolates (260) were NIV chemotype；9 isolates were identified as DON chemotype by Tri13 but as either NIV chemotype or unknown by Tri7. The result of chemical analysis also supported the chemotype determination；all of the NIV chemotype isolates produced NIV, whereas the 9 isolates produce either DON or no toxin.

• Journal of Microbiology and Biotechnology
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• 제24권6호
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• pp.732-739
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• 2014

We describe the development of a polymerase chain reaction method for the rapid, precise, and specific detection of the Xanthomonas oryzae pv. oryzae (Xoo) K3a race, the bacterial blight pathogen of rice. The specific primer set was designed to amplify a genomic locus derived from an amplified fragment length polymorphism specific for the K3a race. The 1,024 bp amplicon was generated from the DNA of 13 isolates of Xoo K3a races out of 119 isolates of other races, pathovars, and Xanthomonas species. The assay does not require isolated bacterial cells or DNA extraction. Moreover, the pathogen was quickly detected in rice leaf 2 days after inoculation with bacteria and at a distance of 8 cm from the rice leaf 5 days later. The results suggest that this PCR-based assay will be a useful and powerful tool for the detection and identification of the Xoo K3a race in rice plants as well as for early diagnosis of infection in paddy fields.

• International Journal of Industrial Entomology and Biomaterials
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• 제17권2호
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• pp.211-215
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• 2008

To select entomopathogenic fungi controlling aphids effectively, several isolates were screened against second instars of Myzus persicae nymphs in the glasshouse using conidia suspension at $1.0{\times}10^5$ conidia/ml. Among these isolates, SFB-205 conidia showed the highest insecticidal activity about 32.7% efficacy to M. persicae at 4 days after application in the glasshouse. The attachment of SFB-205 conidia on the surface of M. persicae nymphs, and germination and penetration were observed using scanning electron microscopy. SFB-205 was identified as Beauveria bassiana species through the comparison of 5.8 s rRNA genes. There were 24 polymorphisms between SFB-205 and the previously reported isolate, B. bassiana ATCC74040 using six kinds of primer combinations in amplified fragment length polymorphism (AFLP) analysis. The B. bassiana SFB-205 might be used as a practical biological control agent for the green peach aphid, M. persicae in the field.

• 식물병연구
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• 제22권4호
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• pp.236-242
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• 2016

Bacterial wilt caused by Ralstonia solanacearum is one of the most devastating diseases in major Solanaceae crops. The pathogen is easily disseminated and survives for many years in plant farming system. Although chemicals are applied to control the disease, they are of limited efficacy and cause several problems. Therefore, the use of phage therapy has been suggested to control the disease as a biological agent. In this study, we discovered bacteriophages lysing diverse Ralstonia isolates from plant and soil samples obtained from the potato cultivated field in Jeju. Three times repeated pickings of plaques resulted in obtaining 173 single phages showing diverse spectrum of host-specificity. With the results, 12 core phages were selected and dendrogram was generated. Genetic diversity of the selected phages was also confirmed by AFLP (Amplified Fragment of Length Polymorphism) fingerprinting. The stability of the phages was investigated in various temperatures and various conditions of pH in vitro. The phages were stable at $16^{\circ}C-44^{\circ}C$ and pH 6-10. Morphological characterization of the phages revealed they were all classified into the Podoviridae, but had diverse head sizes. The results of this research will contribute to control the disease and further researches regarding genetic and molecular aspects will facilitate understanding phage and bacteria interaction.

• 식물분류학회지
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• 제42권3호
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• pp.215-221
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• 2012

5개의 한국산 새우난초류 즉 새우난초, 한라새우난초, 금새우난초, 여름새우난초, 신한새우난초의 분류학적 및 유전적 유연관계가 증폭길이다형성(AFLP) 자료를 근거로 조사되었다. 위의 5개 분류군 및 노란색에 흰 설엽을 갖는 몇몇 돌엽변이체 등 16 개체가 연구에 사용되었다. AFLP를 통해서 50개의 분자마커가 동정되었다. 다변량분석의 결과는 유전적 다양성이 종내에서 보다 종간에 크게 나타났다. 유전적 비유사성은 다른 분류군에 비해서 YW 돌연변이체들 간에 가장 낮았고, 여름새우난초 개체들 간에 가장 높았다. 돌연변이체들은 주된 유집군의 밖에 유집되었다. 한라새우난초는 새우난초와 먼저 유집되어 유전적 구성이 다른 종보다 새우난초에 더 가까운 것으로 판단된다. 비록 한라새우난초가 새우난초와 금새우난초의 자연잡종에 의해 기원되었다고 하여도 분자 마커, 유집분석 그리고 비유사도 자료를 통해서 유전자이입이 새우난초 쪽으로 이루어진 것으로 판단된다. 결론적으로 한국산 새우난초류는 유전적 다양성이 있고, 한라새우난초는 새우난초 쪽으로 유전자이입이 있으며, YW 돌연변이체들은 유전적으로 금새우난초에 보다 가깝다.

• Plant Biotechnology Reports
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• 제5권3호
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• pp.265-271
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• 2011

The cultivated potato as well as its tuber-bearing relatives are considered model plants for cell and tissue culture, and therefore for exploiting the genetic variation induced by in vitro culture. The association between molecular stability and tissue culture in different genetic backgrounds and ploidy levels has already been explored. However, it still remains to be ascertained whether somaclonal variation differs between callus-derived chromosome-doubled and undoubled regenerants. Our research aimed at investigating, through amplified fragment length polymorphism (AFLP) markers, the genetic changes in marker-banding patterns of diploid and tetraploid regenerants obtained from one clone each of Solanum bulbocastanum Dunal and S. cardiophyllum Lindl (both 2n = 2x = 24) and tetraploids from cultivated S. tuberosum L. (2n = 4x = 48). Pairwise comparisons between the banding patterns of regenerants and parents allowed detecting considerable changes associated to in vitro culture both at diploid and tetraploid level. The percentages of polymorphic bands between diploid and tetraploid regenerants were, respectively, 57 and 69% in S. bulbocastanum and 58 and 63% in S. cardiophyllum. On average, the frequencies of lost parental fragments in regenerants were significantly higher than novel bands both in S. bulbocastanum (48 vs. 22%) and S. tuberosum (36 vs. 18%) regenerants. By contrast, in S. cardiophyllum, a similar incidence of the two events was detected (32 vs. 29%). Our results revealed that structural changes after tissue culture process strongly affected the genome of the species studied, but diploid and tetraploids regenerated plants responded equally.

• 대한의생명과학회지
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• 제16권2호
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• pp.75-82
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• 2010

The molecular epidemiological characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolates have demonstrated their genetic diversity and evolution. A total of 137 strains of MRSA clinical isolates was collected from Korean healthcare facility in 2007. The MRSA clinical isolates were analyzed by molecular typings (SCCmec element and agr locus typing), virule nce factor gene detections {(Panton-Valentine leukocidin (PVL), enterotoxin, exfoliative toxin and toxic shock syndrome toxin-1), and amplified fragment length polymorphism (AFLP)}. The MRSA clinical isolates were classified as SCCmec type II-agr type 1 (2 strains), type II-agr type 2 (79 strains), type III-agr type 1 (24 strains), type III-agr type 2 (2 strains), type IV-agr type 1 (27 strains), type IV-agr type 2 (2 strains), and non-typable (1 strain, agr type 3). Based on SCCmec types, SCCmec type II (95.1%) and III (88.5%) indicated higher multidrug resistance rate than SCCmec type IV (10.3%) (P<0.001). The most common enterotoxin genes were seg (83.8%), sei (83.1%), and sec (80.2%). The tst gene was present in 86 out of 137 (62.8%) MRSA isolates. All MRSA isolates were negative for PVL and exfoliative toxin genes. The combinations of toxin genes were observed in particular SCCmec types; 97.6% of SCCmec type II strains carried sec, seg, sei and tst genes, 73.0% of SCCmec type III strains carried sea gene, and 89.7% of SCCmec type IV strains carried sec, seg and sei genes. Each of the SCCmec types of MRSA isolates had distinct AFLP profile. In conclusion, SCCmec type II, agr type 1 and 2 have demonstrated to be the most common types in Korea, and the results indicated that the virulence factors are closely associated with their molecular types (SCCmec and agr types).