International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Identification of an Entomopathogenic Fungus, Beauveria bassiana SFB-205 toxic to the Green Peach Aphid, Myzus persicae

  • Kim, Jae-Su (AgroLife Research Institute (ARI), Dongbu HiTek Co. Ltd.) ;
  • Roh, Jong-Yul (Department of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University) ;
  • Choi, Jae-Young (Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Shin, Sang-Chul (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute) ;
  • Jeon, Mun-Jang (Department of Forest Resources, College of Life and Environmental Science, Daegu University) ;
  • Je, Yeon-Ho (Department of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University)
  • Published : 2008.12.31
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Abstract

To select entomopathogenic fungi controlling aphids effectively, several isolates were screened against second instars of Myzus persicae nymphs in the glasshouse using conidia suspension at $1.0{\times}10^5$ conidia/ml. Among these isolates, SFB-205 conidia showed the highest insecticidal activity about 32.7% efficacy to M. persicae at 4 days after application in the glasshouse. The attachment of SFB-205 conidia on the surface of M. persicae nymphs, and germination and penetration were observed using scanning electron microscopy. SFB-205 was identified as Beauveria bassiana species through the comparison of 5.8 s rRNA genes. There were 24 polymorphisms between SFB-205 and the previously reported isolate, B. bassiana ATCC74040 using six kinds of primer combinations in amplified fragment length polymorphism (AFLP) analysis. The B. bassiana SFB-205 might be used as a practical biological control agent for the green peach aphid, M. persicae in the field.

Keywords

References

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