Browse > Article

Analysis of Genetic Relationship of Pear (Pyrus spp.) Germplasms Using AFLP Markers  

Cho, Kang-Hee (National Institute of Horticultural & Herbal Science, RDA)
Shin, Il Sheob (National Institute of Horticultural & Herbal Science, RDA)
Kim, Hyun Ran (National Institute of Horticultural & Herbal Science, RDA)
Kim, Jeong-Hee (National Institute of Horticultural & Herbal Science, RDA)
Heo, Seong (National Institute of Horticultural & Herbal Science, RDA)
Yoo, Ki Yeol (National Institute of Horticultural & Herbal Science, RDA)
Publication Information
Korean Journal of Breeding Science / v.41, no.4, 2009 , pp. 444-450 More about this Journal
Abstract
Amplified fragment length polymorphism (AFLP) marker was utilized for evaluation of genetic diversity of 60 pear germplasms. Twenty selective AFLP primer pairs generated a total of 522 polymorphic amplification products. From UPGMA (unweighted pair-group method arithmetic average) cluster analysis by using polymorphic bands, the pear germplasms were divided into four clusters by similarity index of 0.691. The first cluster (I) included European pears belonging to Pyrus communis and wild species such as P. nivalis and P. cordata. The second cluster (II) included Ussurian pea pears belonging to P. betulaefolia and P. fauriei. The third cluster (III) included pea pears belonging to P. calleryana and P. koehnei. Most of germplasms belonging to P. pyrifolia and P. ussuriensis, and interspecific hybrids were included in the fourth (IV) cluster. Therefore pear germplasms originated from East Asia were closely related to P. pyrifolia and P. ussuriensis. Similarity values among the tested pear germplasms ranged from 0.584 to 0.879, and the average similarity value was 0.686.
Keywords
Pyrus spp.; AFLP; cluster analysis; genetic diversity;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Bao L, Chen K, Zhang D, Cao Y, Yamamoto T, Teng Y, 2007. Genetic diversity and similarity of pear (Pyrus L.) cultivars native to East Asia revealed by SSR (simple sequence repeat) markers. Genet. Resour. Crop. Evol. 54:959-971   DOI
2 Bell RL. Quamme HA, Layne REC, Skirvin RM. 1996. Pears. In: Janick J, Moore JN (eds), Fruit breeding, Vol Ⅰ: Tree and tropical fruits. John Willey & Sons, London, pp.441-514
3 Cheong KH. 1994. Taxonomic studies on native and cultivated Pyrus species in Korea. Ph.D. Thesis. Seoul National University. pp.76-79
4 Jang JT, Tanabe K, Tamura F, Banno K. 1991. Identification of Pyrus species by peroxidase isozyme phenotypes of flower bud. J. Jpn. Sco. Hort. Sci. 60:513-519   DOI
5 Kimura T, Shi YZ, Shoda M, Kotobuki K, Matsuta N, Hayashi T, Ban Y, Yamamoto T. 2002. Identification of Asian pear varieties by SSR analysis. Breeding Science 52:115-121   DOI   ScienceOn
6 Westwood MN, Challice JS. 1978. Morphology and surface topography of pollen and anthers of Pyrus species. J. Amer. Soc. Hort. Sci. 103:28-37
7 Zhang C, Tanabe K. Wang S, Tamura F, Yosihda A, Matsumoto K. 2006. The impact of cell division and cell enlargement on the evolution of fruit size in Pyrus pyrifolia. Annals of Botany 98:537-543   DOI   ScienceOn
8 Ashok KG, Lewis NL, David MH, Judith NS. 2006. European and Asian pears: simple sequence repeat-polyacrylamide gel electrophoresis-based analysis of commercially important North American cultivars. HortScience 41:304-309
9 Kajiura I, Nakajima M, Sakai Y, Oogaki C. 1983. A species-specific flavonoid from Pyrus ussuriensis Max. and Pyrus aromatica Nakai et Kikuchi, and its geographical distribution in Japan. Jap. J. Breed, 33(1):1-14
10 Kim DI. 1998. Taxonomy of Oriental pear (Pyrus spp.) based on multivariate and RAPD analyses. Ph.D. Thesis. Seoul National University. pp.81-82
11 Shen T. 1980. Pears of China. HortScience 15:13-17
12 Westwood MN. 1968. Comparison of Pyrus fauriei Schneider with P. calleryana Decaisne (Rosaceae). Baileya 16:39-41
13 Iketani H, Manabe T, Matsuta N, Akihama T, Hayashi T. 1998. Incongruence between RFLPs of chloroplast DNA and morphological classification in east Asian pear (Pyrus spp.). Genet. Resour. Crop Evol. 45:533-539   DOI   ScienceOn
14 Chevreau E, Leuliette S, Gallet M. 1997. Inheritance and linkage of isozyme loci in pear (Pyrus communis L.). Theor. Appl. Genet. 94:498-506   DOI   ScienceOn
15 Monte-Corvo L, Goulão L, Oliveira C. 2001. ISSR analysis of cultivars of pear and suitability of molecular markers for clone discrimination. J. Amer. Soc. Hort. Sci. 126:517-522
16 Yamamoto T, Kimura T, Sawamura Y, Kotobuki K, Ban Y, Hayashi T, Mastsuta N. 2001. SSRs isolated from apple can identify polymorphism and genetic diversity in pear. Theor. Appl. Genet. 102:865-870   DOI   ScienceOn
17 Bell RL. 1990. Pears (Pyrus). In: Moore, JN, Ballington JR. (eds), Genetic resources of temperate fruit and nut crops I. International Society for Horticultural Science, Wageningen, Netherlands, pp.665-697
18 Oliveira C, Mota M, Mont-Corvo L, Goulão L, Silva DM. 1999. Molecular typing of Pyrus based on RAPD markers. Sci. Hort. 79:163-174   DOI   ScienceOn
19 Katayama H, Adachi S, Yamamoto T, Uematsu C. 2007. A wide range of genetic diversity in pear (Pyrus ussuriensis var. aromatica) genetic resources from Iwate, Japan revealed by SSR and chloroplast DNA markers. Genet. Resour. Crop Evol. 54:1573-1585   DOI   ScienceOn
20 Monte-Corvo L, Cabrita L, Oliveiria CM, Leitão J. 2000. Assessment of genetic relationship amomg Pyrus species and cultivars using AFLP and RAPD markers. Genet. Resour. Crop Evol. 47:257-265   DOI   ScienceOn
21 Jang JT, Tanabe K, Tamura F, Banno K. 1992. Identification of Pyrus species by leaf peroxidase isozyme phenotypes. J. Jpn. Sco. Hort. Sci. 61:273-286   DOI
22 Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23:4407-4414   DOI   ScienceOn