Proceedings of the Korean Society of Plant Pathology Conference (한국식물병리학회:학술대회논문집)
- 2003.10a
- /
- Pages.123.2-124
- /
- 2003
Genetic diversity of Fusarium graminearum from rice in Korea
- Chang, In-Young (School of Agricultural Biotechnology, Seoul National University) ;
-
Yun, Sung-Hwan
(Division of Life Sciences, Soonchunhyang University) ;
-
Lee, Yin-Won
(School of Agricultural Biotechnology, Seoul National University)
- Published : 2003.10.01
Abstract
Fusarium graminearum (telomorph:Gibberella zeae), an important fungal pathogen of cereal crops with ubiquitous geographic distribution, produces mycotoxins on diseased crops that has threaten human and animal health. Recently severe epidemics of scab diseases of barley and rice by this fungus occurred in Korea, causing serious economic losses. To determine genetic diversity of F. graminearum from rice in Korea, a total of 269 isolates were obtained from Southern part of Korea during 2001-2002. A phylogenetic tree of the isolates was constructed by using amplified fragment length polymorphism (AFLP). Population structure of the rice isolates consists of a single lineage (lineage 6). Frequency of female fertility among these Isolates was relatively low (37%) compared to that among lineage 7 isolates from Korean corn. PCR amplification using chemotype specific primers derived from Tri7 and Tri13 genes at the trichothecene biosynthesis gene cluster revealed that most isolates (260) were NIV chemotype;9 isolates were identified as DON chemotype by Tri13 but as either NIV chemotype or unknown by Tri7. The result of chemical analysis also supported the chemotype determination;all of the NIV chemotype isolates produced NIV, whereas the 9 isolates produce either DON or no toxin.
Keywords