• Journal of the Korean Society of International Agriculture
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• v.30 no.4
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• pp.347-356
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• 2018

Genetically modified (GM) crops have been increased continuously over the world and concerns about the potential risks of GM crops have also been increasing. Even though GM crops have not been cultivated commercially in Korea, it should be necessary to develop the safety assesment technology for GM crops. In this study, we investigated the influence of heading date difference on gene flow from GM to non-GM rice. In the experimental plot design, The PAC GM rice was placed in the center as a pollen donor and non-GM rice were placed in eight directions as pollen receivers. Five pollen receiver rice cultivars were Unkawng, Daebo, Saegyejinmi, Nakdong-byeo, and Ilmi which had different flowering times. A total of 266,436, 300,237, 305,223, 273,373, and 290,759 seeds were collected from Unkawng, Daebo, Saegyejinmi, Nakdong, and Ilmi, respectively, which were planted around PAC GM rice. The GM${\times}$non-GM hybrids were detected by repeated spraying of herbicide and PAT immunostrip assay. Finally, the hybrids were confirmed by PCR analysis using PAC gene specific primer. The hybrids were found in Nakdong-byeo which had the same heading date with PAC GM rice. The hybridization rate was 0.0007% at Nakdong-byeo plot. All of GM${\times}$non-GM hybrids were located within 2 m distance from the PAC GM rice zone. The physiological elements including rice heading date were found to be important factors to determine GM?rice out crossing rate with GM rice. Consideration should be taken into for many factors like the physiological elements of field heading date of rice cultivars to set up the safety management guideline for prevention of GM rice gene flow.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.3
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• pp.279-290
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• 2021

This study evaluated the economic feasibility of facility farms according to the introduction of the ultra-high density pot cultivation (UHDPC) for mango. The basic model was selected as a representative farmhouse case in the Jeonnam region, and seven scenarios for conventional crops were tested. The economic feasibility of crop switching was analyzed through partial budget analysis (PBA). The PBA analysis results were supplemented by adding a sensitivity analysis and a break-even point (BEP) analysis. As a result of the analyses, it was found that crop conversion was economical because the increase in the profit coefficient was greater than the increase in the loss coefficient due to the introduction of mango for all conventional crops. The economics of introducing mango was more sensitive to changes in gross income than to changes in cost items. The results show the detailed conditions of crop selection according to the conditions and preferences of individual farms by presenting the direction of increase and decrease by budget item and information on the amount of increase or decrease. In particular, the BEP analysis result, which is about half of the farms case, is significant in that it presents the minimum target value according to production and market risks.

• Journal of Plant Biology
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• v.36 no.4
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• pp.399-406
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• 1993

유채(Brassica napus L. cv. Topas) 소포자 배양에 의한 기내 배발생 과정을 관찰하고 발생과정에 따른 총단백질의 변화 양상을 이차원 전기영동을 통하여 분석하였다. 배양에 적합한 소포자의 발달 단계는 4,6-diamidino-2-phenylindole (DAPI) 형광염색으로 핵상관찰을 통하여 결정하였다. 최초의 소포자 생존율은 63.9%였으며 이러한 생존율의 차이로 배양초기에 소포자의 이형성이 나타났다. 배 발달 경로에 따른 최초의 세포분열은 화분벽 안에서 균등분열로 시작되어 배병의 발달이 선행된 후 배 발생이 일어났으며, 구형, 심장형, 어뢰형으로의 배 발달은 여러 조직의 분화와 더불어 빠르게 진행되었다. 소포자배 발달과정을 치상단계의 소포자, 배양 3일째의 초기 분열세포, 구형 및 심장형배, 어뢰형배, 성숙한 자엽단계의 배 등의 5단계로 나누어 각각 2차원 전기영동을 수행한 결과, 최초 소포자 단계에서 나타났던 23개의 단백질들은 배 달달 경로로 진행됨에 따라 사라지고, 배양 3일째에는 8개의 단백질이 특이적 또는 지속적으로 발현되었다. 배의 발달과 더불어 20∼50 kD 사이에서 총 42개의 단백질이 급격히 나타나거나 또는 후기 배로 진행하면서 점차적으로 발현되었다.

• Nuclear Engineering and Technology
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• v.13 no.4
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• pp.229-236
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• 1981

The boron content in reactor grade aluminum has been determined by means of a capture gamma-ray counting method. The experimental detection limit is found to be 5.7% ppm with 10% uncertainty. In order to improve the sensitivity, the boron is preconcentrated from aluminum by cation exchange resin system. The accuracies of both methods, i.e., one by the direct measurement and the other by the measurement after preconcentration, are checked by an additive method. The results show good agreements with less than 5% deviation.

• Nuclear Engineering and Technology
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• v.12 no.1
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• pp.29-38
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• 1980

A radiochemical separation scheme for the neutron activation analysis is developed for the determination of 28 elements in aluminum. The scheme is based on a group separation using ion-exchange resin and mineral exchanger. Present work has employed mineral acids and their partly organic mixture excluding HF as the media as well as common glass wares. For the determination, gamma-ray spectroscopy using $3"\times3"\;Nal(TI)$ detector and a single comparator method are used.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2003.11a
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• pp.155-156
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• 2003

대도시내의 산업단지의 경우 주거지역과 인접하여 위치하고 있기 때문에 산업단지에서 발생하는 오염물질들은 시민들의 생활에 큰 영향을 미치고 있다. 그러나 국내에서는 아직 PM10의 장기적인 연구가 다소 미흡하고 가장 오염이 심하리라고 예상되는 산업단지에서 조차 PM10의 장기적인 자료가 없다. 따라서 장기간 자료에 근거한 대기 중 미량금속의 특성평가, 인체 위해도 분석 및 오염원 평가가 원활히 이루어지지 못하고 있는 실정이다. (중략)

• Applied Biological Chemistry
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• v.47 no.4
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• pp.430-433
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• 2004

Lilium cv. Casablanca pollen grains stored at $-70^{\circ}C$ were grown in pollen germination medium with Agrobacterium tumefaciens LBA4404 cells harboring pBI121 for 18 hr at $27^{\circ}C$. Following this, cefotaxime (250 mg/L) was treated for 6 hr to eradicate the bacterial cells. Histochemical GUS analysis revealed that the transgenic pollen displayed deep blue color mostly from 12 hr after the co-cultivation. Presence of $200\;{\mu}M$ acetosyringone was determined not to be more effective for GUS transformation than its absence. GUS DNA integration in the transgenic pollen genomic DNA was clearly demonstrated by Southern blot analysis.

• The Korean Journal of Ecology
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• v.11 no.3
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• pp.137-144
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• 1988

The use of modern pollen spectra for comparative interpretation of fossil pollen sequence requires the ability to distinguish different vegetation types by their pollen spectra, which may show similarities to fossil assemblages. Tertiary marine and non-marine sediments, the Yeonil Group, of the Yeonil area, Korea, was found thirty-two palynomorphic genera. One of the characteristic features of Yeonil geo-flora is predominance of coniferous trees over shrubs, herbs, and ferns. Miocene deposits of Yeonl area, Korea, ae differentiated with five zones. According to the pollen diagram it can be inferred the climate of the Tertiary Miocene of the Yeonil area, Korea.

• Journal of Plant Biology
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• v.30 no.4
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• pp.267-276
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• 1987

A pollen analytical study was carried out on the accumulated humus samples collected from 27 sites under the different plant communities of Mt. Seolag. These pollen spectra were then compared with the actual vegetation. The aboreal pollen(AP) and non-abnoreal pollen(NAP) were 1 Family 25 Genus and 5 Family 5 Genus, respectively. Among the aboreal pollen identified, the pollen of Pinus, Quercus, Betula and Acer were appeared in abundance and the coniferous pollen of Pinus was occupied at least 20%-65% through all the sites studied. The pollens of Abies, Betula, accer, Quercus, Carpinus, Corylus, Fraxinus, Styrax, Prunus and Ericaceae were in accordance with the present vegetation but the other pollens were inconsistent with the actual vegetaton. It suggests that it was caused by the difference between Wehseolag and Namseolag of subalpine zone in Mt. Seolag was 200 m by pollen spectra. Quercus was distributed at the lower part of Namseolag and the subalpine boreal elements were increased with increasing altitudes. The pollen spectra of each site was put together into 5 groups of pollen assemblage by cluster analysis and the community coefficient (CC) was over 60% between all sites studied.

• Korean Journal of Plant Taxonomy
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• v.36 no.3
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• pp.163-187
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• 2006

Taxonomic studies were conducted to evaluate the interspecific relationships in Viola albida var. albida and its related taxa using seven populations for morphology, palynology and anatomy. Molecular phylogenetic studies were also examined in 28 populations including 19 Korean, four Chinese, two Japanese, one American population and two outgroups using nrITS, and 27 populations except V pinnata for trnL-F region of chloroplast DNA. Morphological differences was observed among seven populations of three species in leaf shape, but characters such as serrate number of leaf margins, petal size, pistil shape were showed overlap between populations. Pollen shape of seven populations was monad and grain shape on the polar axis was semi-angular. Morphology of aperture was tri-colporate, and the surface sculpturing was scabrate in rugulate. The grain shape of equatorial view of five populations was prolate whereas V albida var. taknhashii type 1 and V albida var. chaerophylloides type 3 were subprolate. The anatomical characters of rnidvein of leaf, petiole, peduncle, root were also described for the species. The stomatal apparatus of the leaves was observed only in abaxial surface, and the number of stoma per unit ($mm^2$) were abundant in incised than lobate or cleft margin of the leaves. The nrITS analysis shows that V. pinnata and V. dissecta was monophyletic and occupied a basal position in the V. albida var. albida and its related taxa. The other clade including infraspecific populations of V. albida, and V. eizanensis was paraphyletic. The trnL-F noncoding region analysis was similar to the ITS tree. According to the above observations in morphology, palynology, anatomy, and molecular phylogenetic analysis, the significant differences were not found except for leaf shape in Viola albida var. albida and its related taxa, therefore V. albida var. takanhashii and V. albida var. chaerophylloides were considered to be an infraspecific taxa of V. albida var. albida rather than an independent species, subvariety or variety of V. pinnata and V. dissecta.