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Utilization of Pollen Grain from Liluim cv. Casablanca as a Transient Gene Expression Host  

Park, Hee-Sung (Department of Plant Biotechnology, Catholic University of Daegu)
Publication Information
Applied Biological Chemistry / v.47, no.4, 2004 , pp. 430-433 More about this Journal
Abstract
Lilium cv. Casablanca pollen grains stored at $-70^{\circ}C$ were grown in pollen germination medium with Agrobacterium tumefaciens LBA4404 cells harboring pBI121 for 18 hr at $27^{\circ}C$. Following this, cefotaxime (250 mg/L) was treated for 6 hr to eradicate the bacterial cells. Histochemical GUS analysis revealed that the transgenic pollen displayed deep blue color mostly from 12 hr after the co-cultivation. Presence of $200\;{\mu}M$ acetosyringone was determined not to be more effective for GUS transformation than its absence. GUS DNA integration in the transgenic pollen genomic DNA was clearly demonstrated by Southern blot analysis.
Keywords
Lilium cv. Casablanca; pollen; Agrobacterium; transfonnation; GUS histochemistry;
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