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http://dx.doi.org/10.12719/KSIA.2018.30.4.347

Influence of heading date difference on gene flow from GM to non-GM rices  

Oh, Sung-Dug (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
Chang, Ancheol (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Boeun (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
Sohn, Soo-In (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
Yun, Doh-Won (Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, Rural Development Administration)
Publication Information
Journal of the Korean Society of International Agriculture / v.30, no.4, 2018 , pp. 347-356 More about this Journal
Abstract
Genetically modified (GM) crops have been increased continuously over the world and concerns about the potential risks of GM crops have also been increasing. Even though GM crops have not been cultivated commercially in Korea, it should be necessary to develop the safety assesment technology for GM crops. In this study, we investigated the influence of heading date difference on gene flow from GM to non-GM rice. In the experimental plot design, The PAC GM rice was placed in the center as a pollen donor and non-GM rice were placed in eight directions as pollen receivers. Five pollen receiver rice cultivars were Unkawng, Daebo, Saegyejinmi, Nakdong-byeo, and Ilmi which had different flowering times. A total of 266,436, 300,237, 305,223, 273,373, and 290,759 seeds were collected from Unkawng, Daebo, Saegyejinmi, Nakdong, and Ilmi, respectively, which were planted around PAC GM rice. The GM${\times}$non-GM hybrids were detected by repeated spraying of herbicide and PAT immunostrip assay. Finally, the hybrids were confirmed by PCR analysis using PAC gene specific primer. The hybrids were found in Nakdong-byeo which had the same heading date with PAC GM rice. The hybridization rate was 0.0007% at Nakdong-byeo plot. All of GM${\times}$non-GM hybrids were located within 2 m distance from the PAC GM rice zone. The physiological elements including rice heading date were found to be important factors to determine GM?rice out crossing rate with GM rice. Consideration should be taken into for many factors like the physiological elements of field heading date of rice cultivars to set up the safety management guideline for prevention of GM rice gene flow.
Keywords
Genetically modified (GM); gene flow; heading date; rice;
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1 Al-Ahmad, H., and J. Gressel. 2006. Mitigation using tandem construct containing a selectively unfit gene precludes establishment of Brassica napus transgenes in hybrids and backcrosses with weedy Brassica rapa. Plant Biotech. 4:23-33.   DOI
2 Bae, H.K., M.M. Oo, J.E. Jeon, D.N. Tien, S.A. Oh, S.D. Oh, S.H. Kweon, M.Y. Eun, and S.K. Park. 2013. Evaluation of gene flow from GM to Non-GM rice. Plant Breeding and Biotech. 1:162-170.   DOI
3 Baek, H.J., S.I. Sohn, M.R. Cho, G.S. Lee, Y.J. Oh, J.S. Park, K.J. Lee, S.D. Oh, S.C. Suh, and T.H. Ryu. 2010. Development of protocol for analyzing pollinator insect-mediated gene transfer from gm crop. Korean J. Intl. Agri. 22:293-297.
4 Baek, H.J., S.Y. Won, J.K. Kim, S.I. Sohn, K. Lee, M.R. Cho, J.K. Song, M.S. Yoon, J. Lee, Y.M. Jin, and T.H. Ryu. 2008. Study on the gene introgression from GM Chinese cabbage to major crops in Cruciferae. Korean J. Intl. Agri. 20:124-129.
5 Chen, L.J, D,S. Lee, Z.P. Song, H.S. Suh, and B.R. Lu. 2004. Gene flow from cultivated rice (Oryza sativa) to its weedy and wild relatives. Ann. Bot. 93:67-73.   DOI
6 Conner, A., T. Glare, and J. Nap. 2003. The release of genetically modified crops into the environment - Part II. Overview of ecological risk assessment. Plant J. 33: 19-46.   DOI
7 Ellstrand, N., H. Prentice, and J. Hancock. 1999. Gene flow and introgression from domesticated plants into their wild relatives. Annu. Rev. Ecol. Syst. 30:539-563.   DOI
8 Gealy, D.R., D.H. Mitten, and J.N. Rutger. 2003. Gene flow between red rice (Oryza sativa) and herbicide-resistant rice (O. sativa): implications for weed management. Weed Technol. 17:627-645.   DOI
9 Raybould, A., and A. Gray. 1993. Genetically-modified crops and hybridization with wild Relatives - a Uk perspective. J. Appl. Ecol. 30:199-219.   DOI
10 Owen, M.D.K. 2000. Current use of transgenic herbicide-resistant soybean and corn in the USA. Crop Pro. 19:765-771.   DOI
11 Shivrain, V.K., N.R. Burgos, M.A. Sales, A. Mauromoustakos, D.R. Gealy, K.L. Smith, H.L. Black, and M. Jia. 2009. Factors affecting the outcrossing rate between $Clearfield^{TM}$ rice and red rice (Oryza sativa). Weed Sci. 57:394-403.   DOI
12 Kim, C.G., K.W. Park, B. Lee, D.I. Kim, J.Y. Park, H.J. Kim, J.E. Park, J.H. An, K.H. Cho, S.C. Jeong, K.H. Choi, J.H. Harn, and H.M. Kim. 2009b. Gene flow from genetically modified to conventional chili pepper (Capsicum annuum L.). Plant Sci. 176:406-412.   DOI
13 KBCH. 2018. Stats of GM crops in Korea. KBCH, Daejeon, Korea.
14 Kim, C.G., B. Lee, D.I. Kim, J.E. Park, H.J. Kim, K.W. Park, H. Yi, S.C. Jeong, W.K. Yoon, C.H. Harn, and H.M. Kim. 2008. Detection of gene flow from GM to non-GM watermelon in a field trial. J. Plant Bio. 51:74-77.   DOI
15 Kim, C.G., D.I. Kim, H.J. Kim, J.Y. Park, B. Lee, K.W. Park, S.C. Jeong, K.H. Cho, J.H. An, K.H. Cho, Y.S. Kim, and H.M. Kim. 2009a. Assessment of gene flow from genetically modified anthracnose-resistant chili pepper (Capsicum annuum L.) to a conventional crop. J. Plant Bio. 52:251-258.   DOI
16 Kim, K.U., J.H. Lee, I.J. Lee, D.H. Shin, and S.U. Kim. 2004. Hybridization of LM(Living Modified) Rice with Weedy Rices. Kor. J. Weed Sci. 24:51-55.
17 Kim, Y.J., K.H. Nam, I.S. Pack, J.H. Park, S.C. Jeong, C.H. Harn, and C.G. Kim. 2014. Gene flow from GM cabbage to Non-GM control. CNU J. Agri. Sci. 41:157-161.
18 Langevin, S.A., K. Clay, and J.B. Grace. 1990. The incidence and effects of hybridization between cultivated rice and its related weed red rice (Oryza sativa L.). Evolution. 44:1000-1008.   DOI
19 Warwick, S.I., H.J. Beckie, and L.M. Hall. 2009. Gene flow, invasiveness, and ecological impact of genetically modified crops. The Year in Evolutionary Biology 2009: Annals of the New York Academy of Sciences 1168:72-99.
20 Song, Z.P., B.R. Lu, and J.K. Chen. 2004. Pollen flow of cultivated rice measured under experimental conditions. Biodivers. Conserv. 13:579-590.   DOI
21 Lee, Y.H., K.S. Kim, Y.S. Jang, H.J. Cho, J.K. Bang, and S.J. Suh. 2009. Pollen-mediated gene flow from glufosinate resistant transgenic oilseed rape (Brassica napus L.) into its wild relatives. Korean J. Intl. Agri. 21:193-198.
22 Lee, B.C., G. Kim, J.Y. Park, H. Yi, K.W. Park, S.C. Jeong, W.K. Yoon, J.H. An, K.H. Cho, and H.M. Kim. 2007. Survey of herbicide resistant oilseed rapes around the basin of rivers in Incheon harbor area. Korean J. Weed Sci. 27:29-35.
23 Jamse C. 2018. Global status of commercialized biotech/GM crops: 2017, ISAAA BRIEF No. 53. ISAAA: Ithaca, NY.
24 Lee, B., J.H. Kim, S.I. Sohn, S.J. Kweon, Y.S. Chung, and S.M. Lee. 2015. Influence of insect pollinators on gene transfer from GM to non-GM soybeans. Korean Journal of Agri. Sci. 42:159-165.
25 Lee, B.K., K.W. Park, C.G. Kim, H.G. Kang, H.J. Sun, Y.I. Kown, I.J. Song, T.H. Ryu, and H.Y. Lee. 2014. Environmental monitoring of herbicide tolerant genetically modified zoysiagrass (Zoysia japonica) around confined field trials. Weed and Turfgrass Sci. 3:305-311.   DOI
26 Lee, B.K., and S.C. Suh. 2011. A study on the trends and biosafety assessment of genetically modified crops. Research of Environ. Law. 33:1-25.
27 Messeguer, J., C. Fogher, E. Guiderdoni, V. Marfa, M. Catala, G. Baldi, and E. Mele. 2001. Field assessments of gene flow from transgenic to cultivated rice (Oryza sativa L.) using a herbicide resistance gene as tracer marker. Theor. Appl. Genet. 103:1151-1159.   DOI
28 Oh, S.D., M.H. Lim, T.H. Ryu, S.I. Shon, S.Y. Park, and K.J. Lee. 2014. Assessment of gene flow in disease resistant (OsCK1) genetically modified rice. Korean J. of Breeding Sci. 46:44-51.   DOI
29 Messeguer, J., V. Marfa, M. Catala, E. Guiderdoni, and E. Mele. 2004. A field study of pollen-mediated gene flow from Mediterranean GM rice to conventional rice and the red rice weed. Mole. Breeding 13:103-112.   DOI
30 OECD. 1999. Consensus document on the biology of Oryza sativa (Rice). Paris: Organization for Economic Cooperation and Development
31 Oh, S.D., D.W. Yun, S.I. Shon, S.K. Park, and A.C. Chang. 2017. Assessment of gene flow from insect-resistant genetically modified rice (Agb0101) to non-GM rice. Korean J. Breeding Sci. 49:180-189.   DOI