• 동의생리병리학회지
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• 제23권5호
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• pp.980-985
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• 2009

The study was designed to test anti-platelet effect and find out anti-platelet mechanism of extract from Gamisopunghwalheol-tang in vitro. The extract was investigated for the inhibition against the aggregation of human platelet suspensions induced from collagen by aggregometer. And also the extract was investigated for the inhibition against the aggregation of human platelet suspensions who is taking aspirin or clopidogrel induced from collagen by aggregometer. In collagen-induced platelet aggregation test, the extract significantly inhibited collagen-induced platelet aggregation in a concentration-dependent manner(p<0.05). The extract significantly inhibited collagen-induced platelet aggregation of human platelet who is taking aspirin or clopidogrel ing 0 mg/ml concentration(p<0.05). And the extract inhibited more ingpatients who is taking aspirin. These results show that the extract from Gamisopunghwalheol-tang has anti-platelet aggregation effect.

• Biomolecules & Therapeutics
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• 제6권4호
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• pp.358-363
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• 1998

Intravenous lipid emulsion is used extensively as a major component of parenteral nutrition for patients in the surgical intensive care unit. Abnormal cardiovascular function related to lipid infusion has been reported although conflicting results exist. In the present study, we investigated the effects of intravenous emulsions of long-chain triglyceride (LCT) and medium-chain triglyceride (MCT) on myocardial ischemia/ reperfusion injury and on platelet aggregation in rat. There was no difference between LCT and MCT considering the effects on left ventricular developed pressure (LVDP) and coronary flow rate (CFR) before and after ischemia/reperfusion in isolated rat heart. On the other hand, a difference was found between LCT and MCT with regard to their effects on heart rate (HR) and end diastolic pressure (EDP) after ischemia/reperfusion. After ischemia/reperfusion, HR was significantly (P<0.05) reduced and EDP significantly (P<0.05) inc.eased by LCT (18$\pm$2.0% and 42.8$\pm$8.9%, respectively), but not by MCT Ex vivo platelet aggregation induced by collagen was reduced by LCT infusion, but not by MCT These findings suggest that MCT may have slightly more favorable effect than LCT on the myocardial function after ischemia/reperfusion in rat.

• Journal of Nutrition and Health
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• 제46권4호
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• pp.324-331
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• 2013

We investigated dietary effects of green tea powder (GTP) on plasma lipids, platelet aggregation, hemolysis, plasma TBARS, and liver enzymes. Thirty one volunteer diving women living on Jeju island consumed 4 g green tea powder daily for a period of four weeks and data for the study subjects were analyzed on the basis of diagnostic criteria for blood pressure (BP)(${\geq}$ 140/90 mmHg), plasma total cholesterol (TC)(${\geq}$ 200 mg/dL), and triglyceride (TG)(${\geq}$ 150mg/dL). Subjects with high BP had significantly higher TC and TG than those with normal BP. Subjects with higher TC had higher TG, and those with higher TG had lower HDL cholesterol. Platelet aggregation in the initial slope was significantly higher in subjects with normal BP, normal TC, or normal TG than their counterparts in high BP, TC, and TG. HDL cholesterol after GTP intake increased only in subject groups with normal BP, normal TC, or normal TG, and plasma TG after GTP intake decreased only in groups with higher BP, higher TG, or higher TC. Plasma TC and TG in subjects with normal BP increased after GTP intake. GTP intake caused a decrease in the initial slope of platelet aggregation in all subject groups with little effect on maximum aggregation. Total bilirubin showed a significant increase and GOT increased in all subject groups after GTP intake. Beneficial effects of short term intake of green tea powder might differ depending on the subject conditions in terms of blood pressure, plasma lipids, and other cardiovascular conditions. However, with the hypolipidemic, antithrombotic, and antioxidant actions of its bioactive flavonoids, long term usage of GTP or brewed green tea may provide preventive effects against cardiovascular disease.

• 한국식품영양과학회지
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• 제35권6호
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• pp.713-720
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• 2006

식물자원으로부터 생리활성 물질의 이용측면에서 국내에서 자생하고 있는 광나무 잎을 대상으로 하여 다양한 생리활성을 가지는 것으로 보고된 페놀성 화합물의 분포를 확인하였다. 총 페놀성 함량은 $0.89{\sim}1.53%$, 축합형 탄닌은 $0.10{\sim}0.13%$였으며, flavonoid는 aglycone인 apigenin, luteolin과 칼럼크로마토그라피를 행하여 분획으로 분리한 후 가수분해하여 이들의 배당체를 확인하였다. 페놀산의 분획 중 유리형 페놀산 화합물에는 tyrosol, t-cinnamic acid, p-Hydroxybenzoic acid, vanillic acid, shikimic acid, protocatecuic acid등이 존재하였다. 에스테르형 페놀산 화합물에는 tyrosol, t-cinnamic acid, ferulic acid, esculetin, caffeic acid, p-coumaric acid, hydroxytyrosol 등이 함유되었다. 결합형 페놀성 화합물에는 tyrosol, t-cinnamic, p-coumaric acid와 미확인 페놀성 물질들이 존재하였다. 따라서 광나무 잎은 xanthine oxidase 활성저해, LDL 산화, 혈소판 응집저해, 항균활성 등 다양한 생리활성을 나타내는 것으로 보고되고 있는 tyrosol, hydroxytyrosol, protocatecuic acid 등 풍부한 페놀성 화합물을 함유하고 있으므로 유용한 식물자원이 될 수 있음을 확인하였다.

• 동의생리병리학회지
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• 제25권3호
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• pp.471-481
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• 2011

Clove has been frequently used as anti-diabetic, anti-microbial, anti-inflammatory, anesthetic drug and remedies for stomachache by coldness. In this study, the antioxidant activity of extract from Clove was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on $Cu^{2+}$-induced human LDL oxidation. Anti-platelet aggregation and anti-thrombotic effects of Clove extracts were studied ex vivo methods by mesuring the inhibitory effect on thrombin induced platelet aggregation and the fibrinolytic activity. The Clove extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation in vitro. Moreover Clove extracts were exhibited remarkable inhibitory effect on platelet aggregation and fibrinolytic activity. In conclusion, the Clove extracts have anti-oxidative and anti-atherosclerotic effects in vitro and ex vivo system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.

• 한국자원식물학회지
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• 제19권1호
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• pp.133-138
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• 2006

홍삼 제조공정을 단순화하면서 효율적으로 유효성분을 증대시키기 위해서 4년근 수삼을 구입하여 깨끗이 수세한 후 $96-98^{\circ}C$에 3시간 정도 수증기로 증삼한 후 30시간 정도 열풍건조하여 홍삼제조를 위한 간이법을 개발하였다. 제조한 홍삼으로부터 홍삼엑스($60^{\circ}$ brix 이상)를 제조하였으며 홍삼엑스의 추출수율은 60% 이상이었다. 간이법으로 제조한 홍삼을 재료로 하여 농축한 홍삼엑스의 사포닌 함량을 조사하기 위해서 HPLC를 이용하여 총 사포닌(total saponin)을 분석하였다. 그 결과 PD계 사포닌 중 암세포전이억제효과와 평활근이완작용이 탁월한 ginsenoside-Rg3에는 H사에 비하여 2배 이상 검출되었으며, 간상해 억제작용과 혈소판 응집억제작용이 있는 것으로 알려진 ginsenoside-Rh1종의 경우 3배 이상 검출이 되었다.

• 한국미생물·생명공학회지
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• 제42권3호
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• pp.302-306
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• 2014

이화누룩은 증자하지 않은 쌀을 물에 불린 후 분쇄하고, 이를 솔잎으로 띄워 발효시킨 한국 전통누룩이다. 이화누룩의 기능성을 평가하기 위해 이화누룩의 에탄올 추출물과 이의 순차적 유기용매 분획물을 조제하고 항혈전 활성을 평가하였다. 그 결과, ethylacetate 분획물은 강력한 thrombin time, prothrombin time, aPTT 연장효과를 나타내었으며, 유기용매 분획후의 물 잔류물에서는 강력한 혈소판 응집저해능이 확인되었다. 본 연구결과는 이화누룩의 ethylacetate 분획이 신규의 항혈전제로 사용 가능함을 나타내며, 이는 이화누룩의 항혈전 활성에 대한 최초의 보고이다.

• 혜화의학회지
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• 제5권1호
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• pp.185-198
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• 1996

After evaluation of antithrombotic effect of 40 herbs on platelet aggregation induced by ADP(Adenosine diphosphate), these results were obtained as follows: 1. Crude drugs exerting over 30 % of in Chinemys reevesii (Gray)hibition on platelet aggregation induced by ADP were Ganoderma japonicum (Fr.) Lloyd., Panax ginseng C. A. Mey., Gastrodia elata Bl., Thea sinensis, Chinemys reevesii (Gray), Cuscuta chinensis Lam., Cervus nippon Temminck., Biota orientalis (L.) Endl., Coriolus versicolor, Cinnamomum cassia Presl., Sophora flavescens Ait., Amomum villosum Lour., Carthamus tinctorius L., Rubus chingii Hu., Poria cocos (Schw.) Wolf., Laminana japonica Aresch., Ligustrum lucidum Ait., Angelica sineusis (Oliv.), Cyperus rotundas L., Ginkgo biloba L., Zingiber officinale Rosc., Prunus persica (L.) Batsch., Schizandra chinensis (Turcz.) Baill. and Plantago asiatica L.. 2. Of crude drugs having showed over 50% of inhibitory effect on platelet aggregation, at the concentration of $100{\mu}g/m{\ell}$, the inhibitory rates were 82.2% in Ganoderma japonicum (Fr.) Lloyd., 55% in Panax ginseng C. A. Mey., 50.8% in Gastrodia elata Bl., while at the concentration of $200{\mu}g/m{\ell}$, antithrombotic rates were 89.4% in Ganoderma japonicum (Fr.) Lloyd., 59.2% in Panax ginseng C. A. Mey., 57.9% in Thea sinensis, 52.7% in Gastrodia elata Bl.. These results suggest that the study sholuld be necessary on antithrombotic effect of solvent fractions of Ganoderma japonicum (Fr.) Lloyd., Panax ginseng C. A. Mey., Gastrodia elaha B1. and Thea sinensis and isolation of effective compound from above drugs.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2004년도 춘계학술대회
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• pp.253-257
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• 2004

Platelet aggregation is a complex phenomenon that probably involves several intracellular biochemical pathways. When activated, platelets change shape, aggregate and release the contents of their intracellular granules. The interactions between platelets and blood vessel walls are important in the development of thrombosis and cardiovascular diseases. When blood vessels are damaged, platelet aggregation occurs rapidly to form haemostatic Plugs or arterial thrombi at the sites of vessel injury or in regions where blood flow is disturbed. These thrombi are the source of thromboembolic complications of atherosclerosis, heart attacks, stroke, and peripheral vascular disease. Therefore, the inhibition of platelet function represents a promising approach for the prevention of thrombosis. Plants constitute a rich source of bioactive chemicals such as phenolics, terpenoids and alkaloids. Plant extracts may be an alternative to currently used medicinal source because they constitute a rich source of bioactive chemicals. This study was performed to investigate the antiplatelet activity of extract of Tabebuia impetiginosa Martius ex DC (Taheebo) and find out which fractions to this activity in rabbit platelet. Taheebo was methanol extracted and solvent fractionated in to five fractions (hexane, chloroform, ethylacetate, butanol and water). And each fractions were investigated inhibitory effects on platelet aggregation induced by various agonists using washed rabbit platelets in vitro.

• Journal of Nutrition and Health
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• 제32권5호
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• pp.561-569
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• 1999

In the atherosclerotic subjects, arterial endothelial cell injury and plaque formation are suspected to be strong causable factors in developing acute coronary syndrome, and it was revealed that platelets have a very important role in this case. Women are exposed to atherosclerosis at a different degree after menopause or oral contraception. The purpose of this study was to determine the effects of endogenous and exogenous estrogen on the degree of platelet aggregation in platelet rich plasma(PRP) in twenty nonsmoking healthy Korean women for 12 weeks. The subjects were assigned to three groups: (1) eight women aged 49 to 60(yr) for the postmenopausel(Pst) group, (2) eight, aged 22 to 30(yr) for the premenopausa(Pre) group, (3) four, aged 23 to 30(yr) for the oral contraceptive (OC) group which used triphasic OC formulation. Fasting blood sample were obtained from the subjects, (1) once per 6 weeks in the Pst group, (2) every phase of the menstrual cycle in the Pre group, (3) each once during and after OC administration in the OC group. ADP, collagen and epinephrine were used as aggregating reagents, and platelet aggregation and time(Δt: time reaching to the maximum point of aggregation) in PRP were measured at the maximum point of aggregation in five minutes. All the data were adjusted for dietary effects, personality type and body mass index(BMI) by using analysis of covariation(ANCOVA). Platelet aggregation to ADP and collagen(MADP and MCOLL) were not significantly different among the three groups, and Δt to ADP and collagen(TADP and TCOLL) were not either. But maximum platelet aggregability and Δt to epinephrine(MEPIN and TEPIN) were significantly different among the three groups, and the OC group showed the lowest value (p<0.01). Maxtimum platelet aggregability and Δt during the menstrual cycle were not significantly different in the Pre group. Any other significant differences in the maximum platelet aggregability and Δt were found between oral contraception phase and washing out phase(menstruation) in the OC group. In results, maximum platelet aggregability and aggregation time to ADP and collagen seemed not to be affected by endogenous and exogenous estrogen, even though MEPIN and TEPIN showed significantly low in the OC group among the three groups.