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Screening of Antioxidative, Anti-platelet Aggregation and Anti-thrombotic Effects of Clove Extracts  

Yang, Young-Yi (Department of Diagnostics College of Oriental Medicine, Dongguk University)
Lee, Min-Ja (Institute of Oriental Medicine, College of Oriental Medicine, Dongguk University)
Lee, Hye-Sook (Department of Diagnostics College of Oriental Medicine, Dongguk University)
Park, Won-Hwan (Department of Diagnostics College of Oriental Medicine, Dongguk University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.25, no.3, 2011 , pp. 471-481 More about this Journal
Abstract
Clove has been frequently used as anti-diabetic, anti-microbial, anti-inflammatory, anesthetic drug and remedies for stomachache by coldness. In this study, the antioxidant activity of extract from Clove was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on $Cu^{2+}$-induced human LDL oxidation. Anti-platelet aggregation and anti-thrombotic effects of Clove extracts were studied ex vivo methods by mesuring the inhibitory effect on thrombin induced platelet aggregation and the fibrinolytic activity. The Clove extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation in vitro. Moreover Clove extracts were exhibited remarkable inhibitory effect on platelet aggregation and fibrinolytic activity. In conclusion, the Clove extracts have anti-oxidative and anti-atherosclerotic effects in vitro and ex vivo system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.
Keywords
Clove; oxidative stress; ROS & RNS; antioxidative; anti-platelet aggregation and anti-thrombotic;
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1 Patel, R.P., McAndrew, J., Sellak, H., White, C.R., Jo, H., Freeman, B.A., Darley-Usmar, V.M. Biological aspects of reactive nitrogen species. Biochim Biophys Acta 1411: 385-400, 1999.   DOI   ScienceOn
2 남석현, 강미영. 한약재 열수추출물의 항산화효과 검정. 한국농화학회지 43: 141-147, 2000.
3 Sanchez, C.S., Gonzalez, A.M.T., Garcia-Parrilla, M.C., Granados, J.J.Q., Serrana, H.L.G., Martinez, M.C.L. Different radical scavenging tests in virgin olive oil and their relation to the total phenol content. Anal Chim Acta 593: 103-107, 2007.   DOI   ScienceOn
4 Magalhaes, L.M., Segundo, M.A., Reis, S. Automatic method for determination of total antioxidant capacity using 2,2-diphenyl-1-picrylhydrazyl assay. Anal Chim Acta 558: 310-318, 2006.   DOI   ScienceOn
5 Maisuthisakul, P., Suttajit, M., Pongsawatmanit, R. Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chem. 100(4):1409-1418, 2007.   DOI   ScienceOn
6 Awika, J.M., Rooney, L.W., Wu, X.L., Prior, R.L., Cisneros-Zevallos, L. Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. J Agric Food Chem. 51: 6657-6662, 2003.   DOI   ScienceOn
7 Rice-Evans, C., Miller, N., Paganga, G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 20: 933-956, 1996.   DOI   ScienceOn
8 Yoon, M.A., Jeong, T.S., Park, D.S., Xu, M.Z., Oh, H.W., Song, K.B., Lee, W.S., Park, H.Y. Antioxidant effect of quinoline alkaloid and 2,4-di-tert-butylphenol isolated from Scolopendra subspinipes. Biol Pharm Bull. 29: 735-739, 2006.   DOI   ScienceOn
9 Fogliano, V., Verde, V., Randazzo, G., Ritieni, A. Method for measuring antioxidant activity and its application to monitoring the antioxidant capacity of wines. J Agric Food Chem. 47: 1035-1040, 1999.   DOI   ScienceOn
10 Crow, J.P. Dichlorodihydrofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species. Nitric Oxide, 1: 145-157, 1997.   DOI   ScienceOn
11 Roberta, R.E., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 26: 1231-1237, 1999.   DOI   ScienceOn
12 Yagi, K.A. Simple fluometric assay for lipoperoxide in blood plasma. Biochem Med. 15: 212-216, 1976.   DOI   ScienceOn
13 Astrup, T., Mullertz, S. The fibrin method for estimating of fibrinolytic activity. Arch. Biochem Biophys. 40: 346-351, 1952.   DOI   ScienceOn
14 Gotoh, N., Niki, E. Rates of interactions of superoxide with vitamin E, vitamin C and related compounds as measured by chemiluminescence. Biochem Biophys Acta 1115: 201-207, 1992.   DOI   ScienceOn
15 Halliwell, B., Gutteridge, J.M. Role of free radicals and catalytic metalions in human disease: an overview. Method Enzymol. 186: 1-85, 1990.
16 Nagata, N., Momose, K., Ishida, Y. Inhibitory effects of catecholamines and anti-oxidants on the fluorescence reactionof 4,5-diaminofluorescein, DAF-2, a novel indicator of nitric oxide. J Biochem. (Tokyo) 125: 658-661, 1999.   DOI
17 Kujala, T.S., Loponen, J.M., Klika, K.D., Pihlaja, K. Phenolic and betacyanins in red beetroot (Beta vulgaris) root: distribution and effects of cold storage on the content of total phenolics and three individual compounds. J Agri Food Chem. 48: 5338-5342, 2000.   DOI   ScienceOn
18 Miller, N.J., Rice-Evans, C., Davies, M.J., Gopinathan, V., Milner, A.A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci. 84: 407-412, 1993.   DOI
19 Blois, M.S. Antioxidant determination by the use of stable free radical. Nature 26: 1199-1200, 1958.
20 Kim, S.I., Yi, J.H., Tak, J.H., Ahn, Y.J. Acaricidal activity of plant essential oils against Dermanyssus gallinae (Acari: Dermanyssidae). Vet Parasitol. 120: 297-304, 2004.   DOI   ScienceOn
21 Damiani, C.E.N., Rossoni, L.V., Vassallo, D.V. Vasorelaxant effects of eugenol on rat thoracic aorta. Vascul Pharmacol. 40: 59-66, 2003.   DOI   ScienceOn
22 Cooke, S.J., Suski, C.D., Ostrand, K.G., Tufts, B.L., Wahl, D.H. Behavioral and physiological assessment of low concentrations of clove oil anaesthetic for handling and transporting largemouth bass (Micropterus salmoides). Aquaculture 239: 509-529, 2004.   DOI
23 Mytle, N., Anderson, G.L., Doyle, M.P., Smith, M.A. Antimicrobial activity of clove (Syzgium aromaticum) oil in inhibiting Listeria monocytogenes on chicken frankfurters. Food Control. 17: 102-107, 2006.   DOI   ScienceOn
24 Gowda, N.K.S., Malathi, V., Suganthi, R.U. Effect of some chemical and herbal compounds on growth of Aspergillus parasiticus and aflatoxin production. Anim Feed Sci Technol. 116: 281-291, 2004.   DOI
25 Prasad, R.C., Herzog, B., Boone, B., Sims, L., Waltner-Law, M. An extract of Syzygium aromaticum represses genes encoding hepatic gluconeogenic enzymes. J Ethnopharmacol. 96: 295-301, 2005.   DOI
26 Prasad, N.S., Raghavendra, R., Lokesh, B.R., Naidu, KAKA. Spice phenolics inhibit human PMNL 5-lipoxygenase. Prostaglandins Leukot Essent Fatty Acids 70: 521-528, 2004.   DOI   ScienceOn
27 허 준. 동의보감. 1st ed. 법인 문화사. 3588, 2005.
28 Burggraf, D., Vosko, M.R., Schubert, M., Stassen, J.M., Hamann, G.F. Different therapy options protecting microvasculature after experimental cerebral ischaemia and reperfusion. Thromb Haemost. 103(5):891-900, 2010.   DOI
29 Swenson, S., Markland Jr, F.S. Snake venom fibrin(ogen)olytic enzymes. Toxicon. 45(8):1021-1039, 2005.   DOI   ScienceOn
30 한방약리학 교재편찬위원회. 한방약리학. 2nd ed. 신일상사. pp 608-610, 2006.
31 Chen, H., Takahashi, S., Imamura, M., Okutani, E., Zhang, Z.G., Chayama, K., Chen, B.A. Earthworm fibrinolytic enzyme: anti-tumor activity on human hepatoma cells in vitro and in vivo. Chin Med J (Engl). 120(10):898-904, 2007.
32 Mine, Y., Wong, A.H.K., Jiang, B. Fibrinolytic enzymes in Asian traditional fermented foods. Food Res Int. 38(3):243-250, 2005.   DOI   ScienceOn
33 Chaieb, K., Zmantar, T., Ksouri, R., Hajlaoui, H., Mahdouani, K., Abdelly, C., Bakhrouf, A. Antioxidant properties of the essential oil of Eugenia caryophyllata and its antifungal activity against a large number of clinical Candida species. Mycoses 50(5):403-406, 2007.   DOI   ScienceOn
34 Sies, H. Oxidative stress: from basic research to clinical application. Am J Med. 30: 31S-38S, 1991.
35 Wolberg, A.S., Campbell, R.A. Thrombin generation, fibrin clot formation and hemostasis. Transfus Apher Sci. 38(1):15-23, 2008.   DOI   ScienceOn
36 Mizuno, T., Sugimoto, M., Matsui, H., Hamada, M., Shida, Y., Yoshioka, A. Visual evaluation of blood coagulation during mural thrombogenesis under high shear blood flow. Thromb Res. 121(6):855-864, 2008.   DOI   ScienceOn
37 Chudzinski-Tavassi, A.M., Kelen, E.M., Paula-Rosa, A.P., Loyau, S., Sampaio, C.A., Bon, C., Angles-Cano, E. Fibrino(geno)lytic properties of purified hementerin, a metallo-proteinase from the leech Haementeria depressa, Thromb. Haemost 80: 155-160, 1998.   DOI
38 Ge, T., Sun, Z.J., Fu, S.H., Liang, G.D. Cloning of thrombolytic enzyme (lumbrokinase) from earthworm and its expression in the yeast Pichia pastoris. Protein Expr Purif. 42(1):20-28, 2005.   DOI   ScienceOn
39 Hahn, B.S., Wu, S.J., Kim, S.W., Kim, Y.S. Evaluation of anticoagulant fibrinolytic activities from crude extracts of insects. Kor J Pharmacogn. 30: 409-412, 1999.
40 Wolberg, A.S. Thrombin generation and fibrin clot structure. Blood Rev. 21(3):131-142, 2007.   DOI   ScienceOn
41 2009년 사망원인 통계 결과, 통계청.
42 Vranova, E., Inze, D., Van Breusegem, F. Signal transduction during oxidative stress. J Exp Bot. 53(372):1227-1236, 2002.   DOI
43 Magalhaes, A., Magalhaes, H.P.B., Richardson, M., Gontijo, S., Ferreira, R.N., Almeida, A.P., Sanchez, E.F. Purification and properties of a coagulant thrombin-like enzyme from the venom of Bothrops leucurus. Comp Biochem Physiol A Mol Integr Physiol. 146(4):565-575, 2007.   DOI   ScienceOn
44 Oliveira-Carvalho, A.L., Guimaraes, P.R., Abreu, P.A., Dutra, D.L.S., Junqueira-de-Azevedo, I.L.M., Rodrigues, C.R., Ho, P.L., Castro, H.C., Zingali, R.B. Identification and characterization of a new member of snake venom thrombin inhibitors from Bothrops insularis using a proteomic approach. Toxicon. 51(4):659-671, 2008.   DOI   ScienceOn
45 Jones, M.N., Holt, R.G. Activation of plasminogen by Streptococcus mutans. Biochem Biophys Res Commun. 322(1):37-41, 2004.   DOI   ScienceOn
46 Matsubara, K., Hori, K., Matsuura, Y., Miyazawa, K. Purification and characterization of a fibrinolytic enzyme and identification of fibrinogen clotting enzyme in a marine green alga, Codium divaricatum. Comp Biochem Physiol B Biochem Mol Biol. 125(1):137-143, 2000.   DOI   ScienceOn
47 Finkel, T., Holbrook, N.J. Oxidants, oxidative stress and the biology of ageing. Nature 408(6809):239-247, 2000.   DOI   ScienceOn
48 Katsube, T., Imawaka, N., Kawano, Ya., Yamazaki, Y., Shiwaku, K., Yamane, Y. Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food Chem. 97(1):25-31, 2006.   DOI   ScienceOn
49 Verstraeta, M., Lijnen, H.R., Collen, D. Thrombolytic agents in development. Drugs 50: 29-42, 1995.   DOI   ScienceOn
50 Fujita, M., Hong, K., Ito, Y., Fujii, R., Kariya, K., Nishimuro, S. Thrombolytic effect of nattokinase on a chemically induced thrombosis model in rat. Biol Pharm Bull. 18: 1387-1391, 1995.   DOI   ScienceOn
51 Li, F., Dara, G., Jamieson, D.C., Usher, E.L. Gene expression of apolipoprotein(a) within the wall of human aorta and carotid arteries. Atherosclerosis 158(2):303-311, 2001.   DOI   ScienceOn
52 Virag, L., Szabo, E., Gergely, P., Szabo, C. Peroxynitrite induced cytotoxicity: metabolism and opportunities for intervention. Toxocol Lett. 140-141: 113-124, 2003.   DOI
53 Rubbo, H., O'Donnell, V. Nitric oxide, peroxynitrite and lipoxygenase in atherogenesis: mechanistic insights. Toxicology 208: 273-288, 2005.   DOI   ScienceOn
54 Raya, A.A., Raya, S.A. Inflammation: A pivotal link between autoimmune diseases and atherosclerosis. Autoimmun Rev. 5: 331-337, 2006.   DOI   ScienceOn
55 Wrona, M., Patel, K., Wardman, P. Reactivity of 2',7'-dichloro dihydro fluorescein and dihydrorhodamine 123 and their oxidized forms toward carbonate, nitrogen dioxide, and hydroxyl radicals. Free Radic Biol Med. 38(2):262-270, 2005.   DOI   ScienceOn
56 Meng, Q., Lewis, P., Wahala, K., Adlercreutz, H., Tikkanen. Incorporation of esterified soybean isoflavone with antioxidant activity into low density lipoprotein. Biochim Biophys Acta 1438: 369-376, 1996.