• Journal of fish pathology
• /
• v.18 no.2
• /
• pp.179-185
• /
• 2005

In Vitro hemolysis and methemoglobin (MetHb) formation in olive flounder rythrocytes were investigated using potassium permanganate ($KMnO_4$) ranging from 2 to 250 ppm, stabilized chlorine dioxide ($S-ClO_2$)ranging from 3.13 to 400 ppm, formalin (37% formaldehyde) ranging from 31.3 to 2,000 ppm and copper sulphate ($CuSO_4$) ranging from 0.04 to 5 ppm. Remarkable hemolysis was found to be induced at $KMnO_4$ concentrations of 31.3-250 ppm and $CuSO_4$ concentrations of 0.63-5 ppm. On the other hand, MetHb formation could not be found at the same treatment concentrations. It is suggested that the cell-damaging system of $KMnO_4$ may be similar from that of $CuSO_4$ in the erythrocytes of olive flounder. Remarkable hemolysis and MetHb formation were found to be induced at $S-ClO_4$ concentrations of more than 25 ppm and 6.25 ppm, respectively. Only $S-ClO_2$ showed both hemolysis and MetHb formation among the chemicals used in the present study. Formalin did not provoke hemolysis at the highest concentration of 2,000 ppm but induced MetHb formation at ranging from 250 to 2,000 ppm. These findings reveal that the mechanism involved in formalin-induced cell-damaging effects differs from that induced by $S-ClO_2$ to olive flounder erythrocytes compared with $KMnO_4$ and $CuSO_4$.

• Food Science of Animal Resources
• /
• v.25 no.4
• /
• pp.373-382
• /
• 2005

This study was performed to investigate the effect of pork on the cadmium detoxification in rats. Ninety male Sprague-Dawley strains were divided into five stoups based on cadmium treatment(0, 25, 50, 100, 250 ppm) and cadmium intoxication was observed for 8 weeks. During following 8 weeks, casein was replaced by Pork and the effect of pork was compared with casein. During intoxicating, the growth mte was lessened. Feed efficiency ratio was significantly low, as cadmium intake was high Discontinuing cadmium feeding, the body weights were relieve4 Pork-fed groups seemed to have higher body weight than casein-fed groups. During intoxication, hemoglobin and hematocrit were dependent upon cadmium intake. However, they became normal at detocification stage. Cadmium accumulation of the pour-fed group was lower than that of casein. Especially, the factors which affected the cadmium content in kidney were Cd(p<0.001) and $Cd^*pork(p<0.001)$. Metallothionein(MT) was increased with cadmium, and MT was not likely to be affected by pork. This study may provide scientific background of pork for detoxification from heavy metals such as cadmium.

• Tuberculosis and Respiratory Diseases
• /
• v.62 no.6
• /
• pp.516-522
• /
• 2007

Background: Postoperative pulmonary complications are important problems that prolong the length of the hospital stay and increase patient mortality. However, our knowledge of the postoperative factors that increase the risk of these complications is less than complete. Methods: We conducted a prospective study to determine the risk factors for postoperative pulmonary complications. A total of 199 patients were referred to the Pulmonology Department for preoperative pulmonary evaluation. We reviewed the perioperative variables according to the National Surgical Quality Improvement Program (NSQIP). Postoperatively, we collected data on the occurrence of postoperative pulmonary complications and we evaluated the relationship between the perioperative variables and outcomes. Results: Twenty two patients (11%) had pulmonary complications; 6 had respiratory failure, 4 had pneumonia, 13 had pleural effusion and, 2 had atelectasis. Five variables were statistically significantly associated with pulmonary complications on the bivariate analyses. Multiple logistic regression analyses revealed that three of these variables were independently associated with an increased risk of pulmonary complications; a high cardiac risk index (OR 16.5, p=0.002), emergency surgery (OR 10.3, p=0.017), and thoracic/abdominal surgery (OR 3.8, p=0.047). Conclusion: The risk factors for postoperative pulmonary complications are a high cardiac risk index, emergency surgery and thoracic/abdominal surgery.

• Journal of fish pathology
• /
• v.8 no.2
• /
• pp.149-156
• /
• 1995

Physiological activities of E. compressa were examined after oral and intraperitoneal(i.p.) adminstration in young(8g) Israeli and colored carp. Hematological parameters were evaluated to test physiological respose. Anti-bacterial activity was examined by counting the number of bacterial cells in the kidney, and also by measuring the change of agglutinin titers following A. sobriae infection. There was a tendency of increase in E. compressa-fed groups in total protein, albumin and glucose levels. The most marked increase was noted in the group fed with 5% E. compressa. GPT and GOT levels were reduced with the increase of E. compressa concentration. Feeding of E. compressa did not alter haematocrit(Ht) and hemoglobin (Hb) values. The number of A. sobriae was reduced in all groups intraperitoneally treated with variable concentration of E. compressa-extract. The lowest bacterial cells were found at the group intraperitoneally treated with $30{\mu}g$ of E. compressa-extract per g of colored carp($30{\mu}g/g$), indicating that the anti-bacterial activity is maximized at this concentration. The agglutinin titers were elevated in E. compressa extract-treated groups($30{\mu}g/g$) with the maximum value of $6.0{\pm}1.1$. These results indicate that E. compressa adminstration activated physiological response, and triggered a cascade for anti-bacterial reaction.

• Journal of Preventive Medicine and Public Health
• /
• v.35 no.3
• /
• pp.229-235
• /
• 2002

Objectives : To evaluate the effects on the formation of benzidine-hemoglobin, and benzidine metabolite-hemoglobin adducts, caused by pretreatment with the known xenobiotic metabolism effectors, ethanol and phenobarbital, in rats administered Direct Black 38 dye. Methods : The experimental rats were divided into three groups: a control group, an ethanol group and a phenobarbital group. Rats were pretreated with ethanol (1g/kg) or phenobarbital (80mg/kg) 24 hours prior to the oral administration of Direct Black 38 (0.5mmol/kg), with the control group being administered the same amount of distilled water. Blood samples were obtained from the vena cava of 5 rats from each group prior to, and at 30 min, 3h, 5h, 9h, 12h, 24h, 48h, 72h, 96h, and 144h following the oral administration of Direct Black 38. Directly after sampling the blood was separated into hemoglobin and plasma, with the adducts being converted into aromatic amines by basic hydrolysis. Hydrolyzed benzidiene, monoacetylbenzidine and 4-aminobiphenyl were analyzed by reverse-phase liquid chromatography with an electrochemical detector, The quantitative amount of the metabolites was expressed by the hemoglobin binding index (HBI). Results : In the ethanol group, benzidine-, monoacetylbenzidine-, and 4-aminobiphenyl-HBI were increased to a greater extent than those in the control group. These results were attributed to the ethanol inducing N-hydrgxylation, which is related to the formation of the hemoglobin adduct, In the phenobarbital group, all the HBIs, with the exception of the benzidine-HBI, were increased to a greater extent than those of the control group. These results were attributed to the phenobarbital inducing N-hydroxylation related to the formation of the hemoglobin adduct. The N-acetylation ratio was only increased with the phenobarbital pretreatment due to the lower benzidine-HBI of the phenobarbital group compared to these of the control and ethanol groups. The N-acetylation ratios for all groups were higher than f for the duration of the experimental period. Although the azo reduction was unaffected by the ethanol, it was inhibited by the phenobarbital, The ratio of the benzidine-HBI in the phenobarbital group was lower than those of the ethanol the control groups for the entire experiment. Conclusion : Our results indicate that both ethanol and phenobarbital increase the formation of adducts by the induction of N-hydroxylation, but also induced N-acetylation. Phenobarbital decreased the formation of benzidine-HBI due to the decrease of the azo reduction. These results suggest that the effects or ethanol and phenobarbital need to be considered in the biochemical monitoring of Direct Black 38.

• Korean Journal of Environmental Biology
• /
• v.36 no.2
• /
• pp.131-139
• /
• 2018

The warming of water as a result of climate change affects fish habitat. Variations in water temperature affect fish physiology almost totally. The rise in water temperature due to climate change leads to hypoxia following decreased oxygen solubility and decreased binding capacity of oxygen-carrying hemoglobin. This study was conducted to evaluate the health status of Atlantic salmon (Salmo salar) fry at elevated water temperatures($20^{\circ}C$) compared with optimum water temperature ($15^{\circ}C$). The method facilitated the detection of biomarker genes using NGS RNAseq analysis and evaluation of their expression pattern using RT-qPCR analysis. The biomarker genes included interferon alpha-inducible protein 27-like protein 2A transcript variant X3, protein L-Myc-1b-like, placenta growth factor-like transcript variant X1, fibroblast growth factor receptor-like 1 transcript variant X1, transferrin, intelectin, thioredoxin-like, c-type lectin lectoxin-Thr1-like, ladderlectin-like and calponin-1. The selected biomarker genes were sensitive to changes in water temperature based on NGS RNAseq analysis. The expression patterns of these genes based on RT-qPCR were similar to those of NGS RNAseq analysis.

• Korean Journal of Animal Reproduction
• /
• v.27 no.2
• /
• pp.179-185
• /
• 2003

To determine whether the diphtheria toxin-A (DT) gene disrupts development of thymocytes in transgenic animal, the DT-A gene was used for the production of transgenic mice directed by proximal Ick promoter sequences. Two transgenic founder mice that contained several copies of transgene were produced by DNA microinjection and integration of transgene in transgenic mice was confirmed by PCR and Southern blotting analysis. Transgenic $F_1$ and $F_2$ mice were produced by outbreeding of founder and $F_1$ mice to investigate expression of transgene and phenotypes in transgneic mice. Expression of the diphtheria toxin gene was confirmed in thymus, spleen and liver of transgenic mice by RT-PCR. In circulating blood of transgenic mice, lower number of circulating white blood cells and platelets were observed compared with that of normal mice. In addition, transgneic mice had reduced number of circulating peripheral T-cells analyzed by FACS with anti-CD3 antibody. The data in these transgenic mice indicate that DT gene can play a disruptive role in developing thymocytes of transgenic mice resulted in lower number of T-cells that can be applicable to a wide range of tissues in other animals.

• The Korean Journal of Food And Nutrition
• /
• v.11 no.2
• /
• pp.192-199
• /
• 1998

1. 조사 대상자들의 평균 키와 체중은 각각 161.00$\pm$4.15cm, 50.45$\pm$8.15kg이었으며 BMI는 19.81$\pm$3.98, 체지방율은 26.12$\pm$4.12, skinfold thickness는 40.40$\pm$12.56mm로 나타났다. 2. 조사 대상자들의 혈액성상은 적혈구 수, 헤모글로빈, 헤마토크리트치, albumin, globulin, 총 콜레스테롤, LDL-콜레스테롤, HDL-콜레스테롤, 총 중성지방을 측정하였으며 헤마토크리트치를 제외한 나머지 성상들은 모두 정상 범위에 속하였다. 헤마토크리트치는 정상범주에 미달되는 것으로 나타나 이로 말미암아 철분 결핍성 빈형에 노출될 확률이 아주 많음을 보였다. 3. 용돈별로 체지방율을 분류하여 분석한 결과 한달 용돈이 평균 20~30만원을 쓰는 group의 체지방율이 22.00%로서 정상 수준 이었으며, 30만원 이상 사용하는 group은 비만으로 나타났다.(p<0.05) 한편, 그 외의 group들은 체내 체지방율 수준도 정상 수준인 20~25%를 넘어 체중으로 나타났다. 또한 30만원 이상 사용하는 group은 다른 group보다 RBC(million/㎣), Hb(g/이), Hct(%) 모두가 낮게 나타났으며, 빈혈로 판정될 수 있는 수준이었다. 4. 다이어트 빈도별 각 요인들간의 상관관계를 살펴보면 다이어트 시도를 자주 한다고 답변한 group이 거의 실시하지 않는다., 실시해 본 적이 없다라고 대답한 group보다 체중과 BMI가 유의적으로 낮았다.(p<0.05) 그러나 skinfold thickness(mm)와 체지방율은 유의차가 나타나지 않았다. 따라서 비록 다이어트 실시를 자주할 경우 절대적인 체중감량은 감소되지만 체내 지방량이 감소되는 것은 아니라고 판단된다. 5. 다이어트 실시기간에 따라 혈액 성상을 분석한 결과, 4개월 미만 다이어트를 실시하였으나, 1개월 전부터 현재(설문지에 응답한 시점)까지 기간 중에는 다이어트를 실시한 적이 없다고 대답한 group의 Alb(g/dl)과 Glo(g/dl)의 수치가 다이어트를 실시한 적이 없다고 대답한 group에 비해 유의적으로 높게 나타났다.(p<0.05) 6. 체중은 BMI와 양의 상관관계를 나타내었으며, BMI는 견갑골과도 높은 양의 상관관계를 가졌다. 상완부는 견갑골, skinfold thickness(mm), 체지방율과 높은 양의 상관관계를 보였으며, 반면 Hb(g/dl), Hct(%)와는 음의 상관관계를 나타내었다. 견갑골은 BMI 뿐 아니라 skinfold thickness(mm), 체지방율과 양의 상관관계를 나타내었으며, skinfold thickness(mm)는 체지방율과 유의적인 양의 상관관계가 있음이 나타났다. 7. RBC(million/㎣)는 Hb(g/dl) 및 Hct(%)와 유의적인 양의 상관관계를 나타내었으며, Hb(g/dl)도 Hct(%)와 유의적인 양의 상관관계를 나타내었다. Alb(g/dl)은 Glo(g/dl) 및 TCH(mg/dl), HDL(mg/dl)과도 양의 상관관계를 가졌다. Glo(g/dl)은 TCH(mg/dl)과 양의 상관관계를 보였으며, TCH(mg/dl)은 Alb(g/dl), Glo(g/dl)이에도 TTG(mg/dl), LDL-cho(mg/dl) HDL-cho과도 양의 상관관계를 나타내었다. TTG(mg/dl)은 LDL-cho(mg/dl)과는 양의 상관관계를 HDL-cho(mg/dl)과는 음의 상관관계를 나타내었다.

• The Korean Journal of Food And Nutrition
• /
• v.15 no.3
• /
• pp.267-271
• /
• 2002

We have studies in 38 subjects (19 control and 19 experiment) for development of functional food for postpartum body control. Mean age of control and experimental subjects were each other 30.4 and 29.0 years old, and mean body mass index were 20.2 and 21.4. Total weight gain during pregnancy of control and experimental subjects were 14.0kg and 19.8kg. We measured change of weight and liver activity after parturition for 3 months. It shows that weight not reduced until 30 days in postpartum but significantly decreased during 30 days to 90 days. The mean weight of control and experimental subjects were decreased 3.15kg and 6.46kg at 60 days(p<0.05), 4.47kg and 7.44kg at 90 days(p<0.05). In this study, hemoglobin, GOT and CPT level were not changes before and after of experiment, respectively.

• Journal of Aquaculture
• /
• v.16 no.1
• /
• pp.1-7
• /
• 2003

A new technique combining forced-feeding and dorsal aorta cannulation was developed to monitor concentration of nutritions in the blood circulation and their metabolites in rainbow trout. To study the effect of dorsal aorta cannulation on stress, 30 rainbow trout (523$\pm$5.4 g; Mean$\pm$SD) were divided into 6 groups of 5 individuals each. A group was anesthetized and blood samples were taken at 0, 3, 6, 12, 24 or 48 h after dorsal aorta cannulation. Hematocrit peaked at 6 h and returned to 0 values by 12 h after dorsal aorta cannulation. Plasma cortisol and glucose concentrations also peaked at 6 h and returned to 0 values by 48 h after dorsal aorta cannulation. Based on the plasma cortisol and glucose concentrations, the rainbow trout recovered from the operation of dorsal aorta cannulation within 48 h. To compare the patterns of plasma free amino acid concentrations after force-feeding in the fish with dorsal aorta cannulation, 5 dorsal aorta cannulated individuals (511$\pm$6.2 g) were kept in a cage. After 48 h starvation, they were anesthetized and blood samples were taken at 0, 4, 8, 12, 24, 36 or 48 h after forced-feeding. The concentration of all plasma free amino acids, except isoleucine, leucine, phenylalanine, and tryptophan, also peaked at 4 h and returned to 0 values by 24 h after feeding. The combined technique allows forced-feeding and repeated sampling of blood in rainbow trout with minimum stress.