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Development of Modeling System for Assessing Essential Amino Acid Requirements Using Surgically Modified Rainbow Trout  

배승철 (부경대학교 양식학과/사료영양연구소)
옥임호 (부경대학교 양식학과/사료영양연구소)
박건준 (부경대학교 양식학과/사료영양연구소)
김강웅 (국립수산과학원)
최세민 (부경대학교 양식학과/사료영양연구소)
Publication Information
Journal of Aquaculture / v.16, no.1, 2003 , pp. 1-7 More about this Journal
Abstract
A new technique combining forced-feeding and dorsal aorta cannulation was developed to monitor concentration of nutritions in the blood circulation and their metabolites in rainbow trout. To study the effect of dorsal aorta cannulation on stress, 30 rainbow trout (523$\pm$5.4 g; Mean$\pm$SD) were divided into 6 groups of 5 individuals each. A group was anesthetized and blood samples were taken at 0, 3, 6, 12, 24 or 48 h after dorsal aorta cannulation. Hematocrit peaked at 6 h and returned to 0 values by 12 h after dorsal aorta cannulation. Plasma cortisol and glucose concentrations also peaked at 6 h and returned to 0 values by 48 h after dorsal aorta cannulation. Based on the plasma cortisol and glucose concentrations, the rainbow trout recovered from the operation of dorsal aorta cannulation within 48 h. To compare the patterns of plasma free amino acid concentrations after force-feeding in the fish with dorsal aorta cannulation, 5 dorsal aorta cannulated individuals (511$\pm$6.2 g) were kept in a cage. After 48 h starvation, they were anesthetized and blood samples were taken at 0, 4, 8, 12, 24, 36 or 48 h after forced-feeding. The concentration of all plasma free amino acids, except isoleucine, leucine, phenylalanine, and tryptophan, also peaked at 4 h and returned to 0 values by 24 h after feeding. The combined technique allows forced-feeding and repeated sampling of blood in rainbow trout with minimum stress.
Keywords
Essential amino acid; Rainbow trout; Forced-feeding; Dorsal aorta cannulation;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Improved method of dorsal aorta catheterization: haematological effects followed for three weeks in rainbow trout (Salmo gairdneri) /
[ Soivio, A.;K. Westman;K. Nyholm ] / Finnish Fish Res.
2 Utilization of dietary protein by salmonid fish. /
[ Pfeffer, E. ] / Comp. Biochem. and Physiol.
3 Plasma amino acids in rainbow trout fed intact protein or a crystalline amino acid diet /
[ Schuhmacher, A.;C. Wax;J. M. Gropp ] / Aquaculture
4 A new technique of feeding, repeated sampling of blood and continuous collection of urine in white sturgeon. /
[ Deng, D. F.;S. Refsite;G. I. Hemre;C. E. Crocker;H. Y. Chen;J. J. Cech Jr.;S. S. O. Hung ] / Fish physiology and biochemistry   ScienceOn
5 Relationship between plasma amino acids and composition of ingested protein. Ⅱ. A shortened procedure to determine plasma amino acid ratios. /
[ Longnecker, J.B.;N. L. Hause ] / Amer. J. Clin. Nutr.
6 Protein nutrition in fish. Ⅵ. Effects of dietary energy sources on the utilization of proteins by rainbow trout and carp. /
[ Ogino, C.;T. Takeuchi ] / Bull. Jap. Soc. Sci. Fish.
7 The nutritional significance of plasma and uninary amino acids. /
[ Young, V. R.;N. S. Scrimshaw.;E. J. Bigwood(ed.) ] / The Internatinal Encyclopedia of Food and Nutrition
8 Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids /
[ Barton, B. A.;G. K. Iwama ] / Annu. Rev. Fish Dis.
9 Studies on the uptake of 14C-amino acids derived from both dietary 14C-protein and dietary 14C-amino acids by rainbow trout. /
[ Cowey, C. B.;M. J. Walton ] / J. Fish Biol.
10 Plasma catecholamines in resting rainbow trout, salmo gairdneri Richardson, by high pressure liquird chromatography /
[ Woodward, J. J. ] / J. Fish Biol.
11 Arginine requirement and apparent absence of a lysine-arginine antagonist in fingerling channel catfish. /
[ Robinson, E. H.;R. P. Wilson;W. E. Poe ] / Journal of Nutrition
12 Availability and utilization of free lysine in rainbow trout (Oncorhynchus mykiss) 1. Effect of dietary crude protein level. /
[ Rodehutscord, M.;F. Borchert;Z. Gregus;P. Michael;E. Pfeffer ] / Aquaculture
13 /
[ Houston A. H.;C. B. Schreck(ed.);P. B. Moyle(ed.) ] / Methods for Fish Biology
14 Effects of dietary supplementation of lysine cell mass as a fish replacer in juvenile nile tilapia. /
[ Kim, K. W.;J. Y. Lee;S. C. Bai;H. S. Lee ] / J. of Aquaculture   과학기술학회마을
15 Dietary requirements of rainbow trout for tryptophan, lysine and arginine determined by growth and biochemical measurements /
[ Walton, M. J.;C. B. Cowey;R. M. Coloso;J. W. Adron ] / Fish Physiol. Biochem.
16 Plasma amino acid levels in rainbow trout (Oncorhynchus mykiss). /
[ Schulmacher, A.;M. Goldberg;J. Sch<TEX>$\"{o}$</TEX>n;C. Wax;J. M. Gropp ] / EIFAC Workshop on Methodology for Determination of Nutrition Requirements in Fish
17 Effects of altering the proportions of essential-to- nonessential amino acid levess in the rat. /
[ Young, V. R.;J. Zamora ] / J. Nutr.
18 Dietary arginine requirement of juvenile red drum (Sciaenops ocellatus) based on weight gain and feed efficiency. /
[ Barziza, D. E.;J. A. Buentello;D. M. Gatlin ] / J. Nutr.
19 Requirements for lysine and arginine by rainbow trout (Oncorhynchus mykiss) /
[ Kim, K.I.;T.B. Kayes;C. H. Amundson ] / Aquaculture
20 Application of a biochemical method for the estimation of amino acid needs in fish: quantitative arginine requirements of rainbow trout in different salinities. /
[ Kaushik, S. J.;J. E. Hlaver(ed.);I. Tiews(ed.) ] / Fin-fish Nutrition and Fish Feed Technology
21 Plasma amino acid changes in rainbow trout force-fed casein and corresponding amino acid mixture. /
[ Yamada, S.;K. Simpson;Y. Tanaka;T. Katayama ] / Bull. Jap. Soc. Sci. Fish
22 Portal absorption and hepatic uptake of amino acids in rainbow trout force-fed complete diets containing casein or crystalline amino acids. /
[ Murai, T.;H. Ogata;Y. Hirasawa;T. Akiyama;T. Nose ] / Nippon Suisan Gakkaishi
23 Postprandial changes in plasma and liver free amino acids of rainbow trout fed complete diets containing casein /
[ Walton, M.;R. Wilson ] / Aquaculture   ScienceOn
24 Stress and acclimation /
[ Wedemeyer, G. A.;B. A. Barton;D. J. McLeay;Schreck, C. B.(ed.);Moyle, P. B.(ed.) ] / Methods for Fish biology
25 Stress response of olive flounder (Paralichthys olivaceus) and japanese croaker (Nibea japonicaa) to live transportation. /
[ Young-Jin Chang;Jun-Wook Hur;Seung-Hyen Moon;Jung-Uie Lee ] / J. of Aquaculture   과학기술학회마을