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Analysis of Transgenic Mouse, for the Production of Immunodeficiency Animals  

나루세겐지 (충남대학교 동물자원학부, 형질전환복제돼지연구센터)
양정희 (충남대학교 동물자원학부, 형질전환복제돼지연구센터)
이승현 (서울대학교 유전자 이식연구소)
최화식 (김천대학교 임상병리학과)
이성호 (공주대학교 영상보건대학)
박창식 (충남대학교 동물자원학부, 형질전환복제돼지연구센터)
진동일 (충남대학교 동물자원학부, 형질전환복제돼지연구센터)
Publication Information
Korean Journal of Animal Reproduction / v.27, no.2, 2003 , pp. 179-185 More about this Journal
Abstract
To determine whether the diphtheria toxin-A (DT) gene disrupts development of thymocytes in transgenic animal, the DT-A gene was used for the production of transgenic mice directed by proximal Ick promoter sequences. Two transgenic founder mice that contained several copies of transgene were produced by DNA microinjection and integration of transgene in transgenic mice was confirmed by PCR and Southern blotting analysis. Transgenic $F_1$ and $F_2$ mice were produced by outbreeding of founder and $F_1$ mice to investigate expression of transgene and phenotypes in transgneic mice. Expression of the diphtheria toxin gene was confirmed in thymus, spleen and liver of transgenic mice by RT-PCR. In circulating blood of transgenic mice, lower number of circulating white blood cells and platelets were observed compared with that of normal mice. In addition, transgneic mice had reduced number of circulating peripheral T-cells analyzed by FACS with anti-CD3 antibody. The data in these transgenic mice indicate that DT gene can play a disruptive role in developing thymocytes of transgenic mice resulted in lower number of T-cells that can be applicable to a wide range of tissues in other animals.
Keywords
Diphtheria toxin-A; lck promoter; Transgenic mice; T-cells;
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