• Journal of Industrial Convergence
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• v.20 no.7
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• pp.123-129
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• 2022

It was to establish the usage possibility as cosmetic material by testing stimulation of Leuconostoc mesenteroides which were cultured from human scalp's dead skin cell by patching it to the skin in the form of solubilized essence. Cultured L. mesenteroides diluted to 1% concentration and essence which contains it were patched to 31 and 32 participants' back each for 24 hours and established the stimulus figures by the specialists in 30 minutes, 24 hours, and 48 hours after they were taken off. They are both revealed as non-stimuli due to the result of 0.011 and 0 points each and therefore they are safe when used as skin products. Considering the results of this test and the other previous studies which reported the L. mesenteroides as anti-allergic, antioxidant, and anti-inflammatory, L. mesenteroides is usable for cosmetics and worth further research.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.1
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• pp.421-424
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• 2016

This study evaluated the efficacy and the skin safety and stability of a cosmetic that is an applied water extract and fermented hydrolysate of abalone (Haliotis discus hannai Ino), which was produced at the south coast of Korea. In the patch test, purified water, 1,3-butylene glycol and liquid crystal lotion were used as the reference group, and water extract and fermented hydrolysate of abalone and lotion formulation of water extract and fermented hydrolysate of abalone were used as the sample group. The results of patch test showed no irritation on skin among in any of the test groups. Therefore, the water extract and fermented hydrolysate of abalone (Haliotis discus hannai Ino) are safe for human skin.

• 대한임상약리학회:학술대회논문집
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• 1994.12a
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• pp.36-36
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• 1994

• Journal of the Korean Applied Science and Technology
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• v.40 no.3
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• pp.453-461
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• 2023

This study was to verify the skin safety of Coptis Japonica extract(CJE) -containing cosmetics by patching them on sensitive skin and checking its stimulating level. 32 subjects diagnosed with sensitive skin by the result of the lactic acid injury test, were subjected to 20 ㎕ of solubilized essence mixed with 2% CJE on the test subjects' back and closed patched for 24 hours. No stimulus reactions were found by bare eyes and scored 0 stimulus figure points after they were taken off in 30 minutes, 24 hours, and 48 hours. Coptis japonica's medical efficacies such as antioxidant, and anti-inflammatory are expectable to be used as functional cosmetics, and this research would be the foundation of further research into its cosmetical usage.

• Journal of the Korea Convergence Society
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• v.13 no.3
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• pp.105-111
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• 2022

It was to test the stimulability of cosmetic which contains scalp extracted Leuconostoc mesenteroides when it's patched to skin and to investigate safety when it's used as skincare products. Scalp toner that contains L. mesenteroides extracts 1%, and PEG-60 Hydrogenated Castor Oil 0.6% was made and also scalp lotion containing L. mesenteroides extract 3%, Glyceryl Stearate 0.7%. Through the stimulant test of each toner and lotion by patch-testing 32 and 33 participants' backs, safety was verified by the result. Considering the result of antioxidant, anti-allergic, and anti-inflammatory properties of L. mesenteroids reported in previous studies, cosmetics containing these are safe for skin at low concentrations and worth for furthur research as non-stimmulative when used at other cosmetics.

• Journal of the Korean Applied Science and Technology
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• v.35 no.1
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• pp.254-262
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• 2018

It was intended to check a possibility of activity of Psidium guajava leaf extract as anti-oxidant and anti-inflammatory material. Total polyphenol and total flavonoid content of Psidium guajava leaf extract was checked. Cream having inhibitory effect on inflammation through toxicity and NO production inhibition in RAW 264.7 cell was manufactured, and skin safety was evaluated. It was confirmen that total polyphenol and flavonoid content of Psidium guajava leaf extract was 126.4 mg/g and 223.17 mg/g respectively, which was high content. According to the results of checking toxicity through cell viability in RAW 264.7 cell, cytotoxicity was not shown. And NO production indicating inflammatory disease was inhibited concentration-dependently. According to the results of carrying out single patch test after manufacturing the cream containing the Psidium guajava leaf extract, skin irritation did not occur for 24 h to put patch on skin or for 24 h after removing the patch. Putting these results together, it was verified that there was possibility of application as raw materials for cosmetics, which would have anti-oxidant activity owing to the high polyphenol and flavonoid content of Psidium guajava leaf extract and anti-inflammatory material through NO production inhibition.

• Journal of the Korean Applied Science and Technology
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• v.34 no.1
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• pp.41-49
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• 2017

In this study, to evaluate antioxidant activity and safety on skin of Arctium lappa L. root extract, antioxidant activity was understood through total content of polyphenol, total content of flavonoid and DPPH radical scavenging activity, and cytotoxicity for B16F10 melanoma and skin cell protection effect for ultraviolet rays A were confirmed. To verify the application as cosmetic material, the first skin patch test was performed. The result of this experiment showed that as the content of Arctium lappa L. root extract increased, the content of polyphenol and flavonoid increased, and DPPH radical scavenging activity was confirmed. The result of checking cytotoxicity for B16F10 melanoma cells showed that it had low toxicity, and over 80% cell protection effect for ultraviolet rays A was confirmed. In addition, through the first skin patch test, Arctium lappa L. root extract was confirmed to have almost no skin irritation. Through this result, Arctium lappa L. root extract is excellent in skin protection from ultraviolet rays, has low toxicity for skin cells and is safe on skin, so its possibility of being a cosmetic ingredient was verified.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.38 no.1
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• pp.43-50
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• 2012

The safety of cosmetics or cosmetic ingredients on human skin is generally evaluated by visual assessment but some early subtle skin changes may not be noticed by the naked eyes. Thus, the present study was conducted to detect skin reactions induced by mildly irritating cosmetic ingredients by using a laser Doppler perfusion imager (LDPI) method that measures blood flow, a $Vapometer^{(R)}$ that measure strans epidermal water loss (TEWL), and a spectrophotometer that measures the skin color as the erythema values ($a^*$). Visual assessment showed that all tested oils and humectants except propylene glycol belong to the low skin irritation ranges (grades 0+ to 2.9+) while all tested surfactants and propylene glycol belong to the moderate-to strong-skin irritation ranges (grades 3+ to 5+). Among three instrumental methods, TEWL assessment appeared to be more sensitive than spectrophotometric or LDPI method and suitable for the detection of subtle skin response invisible to the naked eye (grades 0+ to 2.9+). Skin reactions of grade 3+ to 5+ could be detected by all three instrumental methods. In conclusion, the current study suggested that the sub-clinical skin reactions due to mild irritants contained in cosmetics can be best assessed by TEWL measurements.

• Clinical and Experimental Pediatrics
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• v.52 no.4
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• pp.499-503
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• 2009

Iodinated contrast media (CM) can cause immediate and late reactions. We treated a patient with a recurrent generalized maculopapular rash and a fever that occurred within two days of exposure to iodinated CM, iopromide ($Ultravist^{(R)}$), for chest computed tomography. We performed skin testing including prick tests, intradermal tests, and patch tests. Our findings indicated a late skin reaction to $Ultravist^{(R)}$in addition to cross-reactions to other iodinated CM such as ioversol ($Optiray^{(R)}$), iohexol ($Iobrix^{(R)}$), and iobitridol ($Xenetix^{(R)}$). In this study, we report the case of a patient diagnosed with a late adverse reaction to $Ultravist^{(R)}$in addition to cross-reactions to other iodinated CM.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.34 no.4
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• pp.303-309
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• 2008

Deficiency of collagen regeneration, denaturalization of elastic fibers, and promotion of reactive oxygen species formation are important factors for deterioration of skin function. They induce wrinckle formation and decrease skin elasticity. Vitamin C and its derivatives have been used as cosmetic ingredients for anti-aging effects but their chemical instability has been a major problem. In an attempt to develop a novel anti-aging agent with a improved stability and bioactivity, the anti-wrinkle effect of 3-O-cetyl-L-ascorbic acid (VCCE), a new vitamin C derivative, was investigated in this study. VCCE increased procollagen type-1 synthesis in a dose-dependent manner (149% at 0.002%) in cultured fiboroblasts. Its potent anti-wrinkle effect was confirmed in vivo by analyzing human skin replica by a visiometer and the PRIMOS system. It did not cause any irritation in human patch test. Taken together, our findings suggest that the VCCE has potential benefits applicable to cosmetics for anti-wrinkle effects.