Journal of Industrial Convergence (산업융합연구)

DAEHAN Society of Industrial Management (대한산업경영학회)
권호 표지
DOI QR코드

DOI QR Code

Clinical research on the skin saftey of the human derived lactic acid culture

인체 유산균 배양액의 피부 안전성 임상 연구

  • Min, Kyung-Nam (Department of Cosmetics inventive Design, Anyang University)
  • 민경남 (안양대학교 화장품발명디자인학과)
  • Received : 2022.06.03
  • Accepted : 2022.07.20
  • Published : 2022.07.28
Download PDF
⟨ Previous Next ⟩

Abstract

It was to establish the usage possibility as cosmetic material by testing stimulation of Leuconostoc mesenteroides which were cultured from human scalp's dead skin cell by patching it to the skin in the form of solubilized essence. Cultured L. mesenteroides diluted to 1% concentration and essence which contains it were patched to 31 and 32 participants' back each for 24 hours and established the stimulus figures by the specialists in 30 minutes, 24 hours, and 48 hours after they were taken off. They are both revealed as non-stimuli due to the result of 0.011 and 0 points each and therefore they are safe when used as skin products. Considering the results of this test and the other previous studies which reported the L. mesenteroides as anti-allergic, antioxidant, and anti-inflammatory, L. mesenteroides is usable for cosmetics and worth further research.

인체의 두피 각질에서 유산균 Leuconostoc mesenteroides을 분리하여 배양하고 이를 함유한 가용화 에센스를 피부에 첩포한 후 자극 유무 검증을 통해 화장품의 소재로 활용 가능성을 판단하고자 하였다. 1% 농도로 희석한 L. mesenteroides 배양액과 이를 1% 함유한 에센스를 제조하여 L. mesenteroides 배양액은 31명, 에센스는 32명의 피시험자들의 등 부위에 24시간 첩포하고 이를 제거한 후 30분, 24시간, 48시간 후의 상태를 피부과 전문의가 육안으로 판독하여 자극 지수를 산출하였다. 이 결과 L. mesenteroides 배양액은 0.011점, 에센스는 0점이 도출되어 모두 무자극으로 피부에 안전한 것으로 나타났다. 본 연구에서의 일차자극시험 결과와 선행 연구에서 보고된 항알레르기, 항산화, 항염 활성능 등을 미루어 보아 저 농도의 L. mesenteroides 배양액은 피부에 안전하여 향후 기초화장품에 적용 시 피부자극이 없는 소재로 활발한 연구가 가능할 것으로 기대된다.

Keywords

Acknowledgement

This paper was supported by the project research funds of the Ministry of SMEs and Startups (MSS) in 2020-2021

References

  1. Foligne, B. et al. (2007). Correlation between in vitro and in vivo immunomodulatory properties of lactic acid bacteria. World J. Gastroenterol, 13, 236-243. DOI : 10.3748/wjg.v13.i2.236
  2. E. Y. Lee. (2008). Problems and verification system of probiotics as livestock-environment improving agent produced and circulated. Microbiology and Biotechnology Letters, 36, 87-95.
  3. M. A. O'Sullivan & C. A. O'Morain. (2000). Bacterial supplementation in the irritable bowel syndrome. A randomised double-blind placebo-controlled crossover study. Dig. Liver Dis, 32, 294-301. DOI : 10.1016/s1590-8658(00)80021-3.
  4. M. S. Ammor & B. Mayo. (2007). Selection criteria for lactic acid bacteria to be used as functional starter cultures in dry sausage production, An update. Meat Sci, 76, 138-146. DOI : 10.12691/jaem-7-1-3
  5. K. H. Lee, Y. J. Bong, H. A. Lee, H. Y. Kim & K. Y. Park. (2016). Probiotic Effects of Lactobacillus plantarum and Leuconostoc mesenteroides Isolated from Kimchi. Journal of the Korean Society of Food Science and Nutrition, 45, 12-19. DOI : 10.3746/jkfn.2016.45.1.012
  6. M. J. Son et al. (2008). Characterization of dextran produced from Leuconostoc citreum S5 strain isolated from Korean fermented vegetable. Eur. Food Res. Techno, 22, 697-706. DOI : 10.1007/s00217-007-0579-y
  7. S. U. Jeong. (2019). Skin Microbiome for Cosmetic Application. Journal of Skin Barrier Research, 21(1), 30-34.
  8. University of Ulsan. (2019). Discovery of pharmabiotics from gut microbiome by immune mechanism study on atopic dermatitis. Ulsan. Ulsan University Industry-University Cooperation Foundation.
  9. K. H. Jeong. (2019). Skin and Intestinal Microbiome in Psoriasis. The Korean Society for Psoriasis, 16(1), 8-13.
  10. A Savadogo, ATC. Ouattar, HN. I. Bassole & S. A. Traore. (2006). Bacteriocins and lactic acid bacteria - a minireview. Afr J Biotechnol. 5, 678-683. DOI : 10.5897/AJB05.388
  11. L. G. Stoianova, E. A. Ustiugova & A. I. Netrusov. (2012). Antibacterial metabolites of lactic acid bacteria: their diversity and properties. Prikl Biokhim Mikrobiol, 48, 259-275.
  12. A. Ljungh & T. Wadstrom. (2009). Lactobacillus molecular biology: from genomics to probiotics. Caister academic Press, 11, 294-295.
  13. LG. Stoianova, EA. Ustiugova & AI. Netrusov. (2012). Antibacterial metabolites of lactic acid bacteria: their diversity and properties. Prikl Biokhim Mikrobiol, 48, 259-275.
  14. G. Tannock. (2005). Probiotics and prebiotics: Scientific Aspects (1st ed.). Caister Academic Press. 217-223. DOI : 10.1155/2008/175285
  15. J. Rafter. (2004). The effects of probiotics on colon cancer development. Nutr Res Rev. 17, 277-284. DOI : 10.1079/NRR200484
  16. Y. Masuda, T. Takahashi, K. Yoshida, Y. Nishitani, M. Mizuno & H. Mizoguchi. (2012). Antiallergic effect of lactic acid bacteria isolated from seed mash used for brewing sake is not dependent on the total IgE levels. Journal of Bioscience and Bioengineering 114(3), 292-296. DOI : 10.1016/j.jbiosc.2012.04.017.
  17. H. S. Lee et al. (2012). Fermenting red ginseng enhances its safety and efficacy as a novel skin care anti-aging ingredient: in vitro and animal study. J Med Food. 15, 1015-1023. DOI : 10.1089/jmf.2012.2187.
  18. B. C. Lee & H. S. Kim. (2021). Antioxidant and Anti-inflammatory Effects of Fermented Eggplant Fruit Extracts with Leuconostoc mesenteroides KD20. Jounal of The Korean Society of cosmetology, 27(5), 1278-1285. https://doi.org/10.52660/JKSC.2021.27.5.1278
  19. J. H. Kang. (2018). Fermentation of Momordica charantia Extract using Leuconostoc mesenteroidesies and Physiological Activity of Product. Journal of the Korean Applied Science and Technology, 35(4), 1250-1259. DOI : 10.12925/jkocs.2018.35.4.1250
  20. C. K. Zhoh, M. H. Lee, I. Y. Kim & J. Y. Shin. (2016). Skin Improvement Effect of Fermented Snail Extract. Journal of the Korean Applied Science and Technology, 33, 30-40. DOI : 10.12925/jkocs.2016.33.1.30j
  21. M. H Han & G. S Moon. (2020). Development of Skin Health Promoting Materials Using Leuconostoc mesenteroides. Microbiology and Biotechnology Letters, 48, 455-462. DOI : 10.48022/mbl.2005.05005
  22. MFDS. (2015). Regulations on Examination of Functional Cosmetics, 2015-14. https://www.mfds.go.kr/.
  23. PCPC. (2014). Safety evaluation guidelines: The Personal Care Products Council, Washington, D.C. 2014.