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http://dx.doi.org/10.12925/jkocs.2017.34.1.41

Antioxidant Activity and Cytotoxicity on cell of Arctium lappa L. root extract  

Moon, Ji-sun (Department of Beauty Health, Jungwon University)
Lee, Jin-Hee (Department of Culture Service, Dongguk University)
You, Seon-hee (Department of Bioengineering, Konkuk University)
Publication Information
Journal of the Korean Applied Science and Technology / v.34, no.1, 2017 , pp. 41-49 More about this Journal
Abstract
In this study, to evaluate antioxidant activity and safety on skin of Arctium lappa L. root extract, antioxidant activity was understood through total content of polyphenol, total content of flavonoid and DPPH radical scavenging activity, and cytotoxicity for B16F10 melanoma and skin cell protection effect for ultraviolet rays A were confirmed. To verify the application as cosmetic material, the first skin patch test was performed. The result of this experiment showed that as the content of Arctium lappa L. root extract increased, the content of polyphenol and flavonoid increased, and DPPH radical scavenging activity was confirmed. The result of checking cytotoxicity for B16F10 melanoma cells showed that it had low toxicity, and over 80% cell protection effect for ultraviolet rays A was confirmed. In addition, through the first skin patch test, Arctium lappa L. root extract was confirmed to have almost no skin irritation. Through this result, Arctium lappa L. root extract is excellent in skin protection from ultraviolet rays, has low toxicity for skin cells and is safe on skin, so its possibility of being a cosmetic ingredient was verified.
Keywords
Arctium lappa L.; Antioxidant; Cosmetics; Cytotoxicity; Skin safety;
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Times Cited By KSCI : 8  (Citation Analysis)
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1 Helmersson J, Arnlov J, Larsson A, Basu S. Low dietary intake of beta-carotene, alpha-tocopherol and ascorbic acid is associated with increased inflammatory and oxidative stress status in a Swedish cohort. Br. J. Nutr. 15, 1-8 (2008).
2 Koskas JP, Cillard J, Cillard P. Autoxidation of linoleic acid and behavior of its hydroperoxides with and without tocopherols. JAOCS. 61, 1467-1472 (1984).
3 Terao, J. Autoxidation activity of ${\beta}$-carotene-related carotenoids in solution. Lipids. 24, 657-661 (1989).
4 Chen YT, Zheng RL, Jia ZJ, Ju J. Flavonoids as superoxide scavengers and antioxidants. Free Radical Biol. Med. 9, 19-21 (1990).
5 Cha, JY, Park SH, Heo JS, Cho YS. Suppressive effect of administrated glutathione-enriched Saccharomyces cerevisiae FF-8 on the oxidative stress in alcoholic fatty liver. J. Life Sci. 18, 1053-1058 (2008).   DOI
6 Lee RJ. edical herbal of Josun. Han Kook Munhwasa, Seoul (1999).
7 Lee MS, Jeong JS, Park DY. The Effects of Arctium lappa L. Root Extracts on the Scalp and hair. Asian Journal of Beauty & Cosmetology. 13, 43-48, (2015).
8 Jeong TS. Study on Microbiological Risk Assessment and Application of HACCP System to Burdock(Arctium lappa L.) Tea Production Process, Catholic university of DAEGU, Doctorate thesis (2016).
9 Lin CC, Lu JM, Yang JJ, Chuang SC, Ujiie T. Anti-inflammatory and radical scavenge effects of Arctium lappa. American J Chin Med, 24, 127-137 (1996).   DOI
10 Kou XS, Dai QW, Luo L, Yin Z. Arctigenin inhibits lipopolysaccharideinduced iNOS expression in RAW264.7 cells through suppressing JAK-STAT signal pathway. Int Immunopharmacol, 11, 1095-1102 (2011).   DOI
11 Weidong. 牛蒡抗氧化. 降血脂的保健功能. 食品科学 34, 274 (2008).
12 Lin, SC, Lin CH, Lin CC, Lin YH, Chen CF, Chen JC, Wang LY. Hepatoprotective effects of Arctium lappa L. on liver injuries induced by chronic ethanol consumption and potentiated by carbon tetrachloride tetrachloride. J. Biomed. Sci. 9, 401-409 (2002).
13 Ryu BH, Lee BH, Ha MS, Kim DS, Sin DB, Nam KD. Desmutagenic effect of legumes and plants crude saponins in Salmonella typhimurium TA 98. Korean J Food Sci Technol 18, 345-350 (1986).
14 Ohara A, Matsuhisa T. Anti-tumor promoting activities of edible plants against okadaic acid. Food Sci Technol Res. 8, 158-161 (2002).   DOI
15 Morita KY, Y. Nishijima and T. Kada. Chemical nature of a desmutagenic factor from burdock (Arctium lappa L.). Agric. Biol. Chem. 49, 925-932 (1985).
16 Sohn EH, Jang SA, Joo H, Park S, Kang SC, Lee CH, Kim SY. Anti-allergic and anti-inflammatory effects of butanol extract from Arctium Lappa L. Clin Mol Allergy, 9, 4-15 (2011).   DOI
17 Chow LW, Wang SJ, Duh PD. Antibacterial activity of burdock. Food Science 24, 195-202 (1997).
18 Duh PD. Antioxidant activity of burdock (Arctium lappa Linne): Its scavenging effect on free-radical and active oxygen. J. Am. Oil. Chem. Soc., 75, 455-461 (1998).   DOI
19 Maruta Y, Kawabata J, Nik R. Antioxidative caffeoylquinic acid derivatives in the roots of burdock (Arctium lappa L.). J. Agric. Food Chem. 43, 2592-2595 (1995).   DOI
20 Lee MY, Shin SL, Park SH, Kim NR, Chang YD, Lee CH. Development of optimal cultivation conditions and analysis of antioxidant activities of Arctium lappa sprout vegetables. Korean. J. Plant. Res., 22, 304-311 (2009).
21 Chen FA, Wu AB, Chen CY. The influence of different treatment on the free radical scavenging activity of burdock and variations of its active components. Food. Chem, 86, 479-484 (2004).   DOI
22 Folin O, Denis W. On phosphotungsticphosphomolybdic compounds as color reagents. The Journal of Biological Chemistry, 12, 239-243 (1912).
23 Moreno MI, Isla MI, Sampietro AR, Vattuone MA. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of Ethnopharmacology, 71, 109-114 (2000).   DOI
24 Blois MS. Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199-1200 (1958).   DOI
25 Guidelines on in vivo tests and in vitro tests of cosmetic products, Korea. food. and drug. adminstr ation. (2011).
26 Im DY, Lee KI. Antioxidative Activity and Tyrosinase Inhibitory Activity of the Extract and Fractions from Arctium lappa Roots and Analysis of Phenolic Compounds. Kor. J. Pharmacogn. 45, 141-146 (2014).
27 Kim EJ, Choi JY, Yu MR, Kim MY, Lee BH. Total Polyphenols, Total Flavonoid Contents, and Antioxidant Activity of Korean Natural and Medicinal Plants. KOREAN J. FOOD SCI. TECHNOL. 44, 337-342 (2012).   DOI
28 Jia Z, Tang M, Wu J. The determination of flavonoid contents in mulberry and they scavenging effects on superoxide radicals. Food Chem. 64, 555-559 (1999).   DOI
29 Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: Chemistry, metabolism, and structure activity reationships. J. Nutr. Biochem. 13, 572- 584 (2002).   DOI
30 Kim MG, Oh MS, Jeon JS, Kim HT, Yoon MH. A Study on Antioxidant Activity and Antioxidant Compound Content by the Types of Tea, Journal of Food Hygiene and Safety. 31, 132-139 (2016).   DOI
31 Yamaguchi T, Mizobuchi T, Kajikawa R, Kawashima H, Miyabe F, Terao J, Takamura H, Matoba T. Radicalscavenging sctivity of vegetables and the effect of cooking on their activity. Food. Sc.i Technol. Res., 7, 250-257 (2001).   DOI
32 Chen FA, Wu AB, Chen CY. The influence of different treatments on the free radical scavenging activity of burdock and variations of its active components. Food Chem. 86, 479-484 (2004).   DOI
33 Lee MS. Antioxidative and Antimutagenic Effects of Arctium lappa Ethanol Extract. Korean J. Food & Nutr. 24, 713-719 (2011).   DOI
34 Jeon SE, Hwang WJ, Hong YH, Kim MJ, Ahn ES, Park SY. Inhibitory Effects of Hericium erinaceus Extracts on Melanin Synthesis and Oxidative Stress. Asian J Beauty Cosmetol. 14, 427-435 (2016).   DOI
35 Lee AR, Roh SS, Lee ES, Min YH. Anti-oxidant and Anti-melanogenic Activity of the Methanol Extract of Pine Cone. Asian J Beauty Cosmetol. 14, 301-308 (2016).   DOI
36 Gu MA, Kim MJ, Kim HS, Ha JH, Yu ER, Park SN. Characteristics and Cellular Protective Effects against UVA of Cationic Liposome Loaded with Quercetin and Rutin. Appl. Chem. Eng., 26, 165-172 (2015).   DOI
37 Kim EJ, Ahn MS. Antioxidative effect of ginger extracts. Korean J. Soc. Food Sci. 9, 37-42 (1993).
38 Aruoma OI, Free radicals. oxidative stress and antioxidants in human health and disease. J. A.O.C.S. 75, 199-212 (1998).
39 Chang SS, Ostric-Matijasevic B, Oliver AL, Huang CL. Natural antioxidants from rosemary and sage. J. Food Sci. 42, 1102-1106 (1977).   DOI
40 Jeong SJ, Lee H, Song NH, Lee SE, Baeg I. Natural products chemistry; screening for antioxidant activity of plant medicinal extracts. J. Korean Soc. Food Sci. Nutr. 33, 28-33 (2004).   DOI
41 Cha JY, Cho YS. Effect of potato polyphenolics on lipid peroxidation in rats. J. Korean Soc. Food Sci. Nutr. 28, 1131-1136 (1999).
42 Farag RS, Badei AZMA, Hewedi FM, EI-Baroty GSA. Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media. JAOCS. 66, 792-799 (1989).   DOI
43 Frei B. Natural antioxidants in human health and disease. Academic Press. San Diego. 40-55 (1994).