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http://dx.doi.org/10.12925/jkocs.2018.35.1.254

Total Phenolic Compound, Total Flavonoid Compound And Anti-Inflammatory Inhibitory Effects of Psidium Guajava Leaf Extract  

Lee, Jeong-Seon (Department of Cosmetology Science, Nambu University)
Kim, Chun-Dug (Department of Cosmetology Science, Nambu University)
Publication Information
Journal of the Korean Applied Science and Technology / v.35, no.1, 2018 , pp. 254-262 More about this Journal
Abstract
It was intended to check a possibility of activity of Psidium guajava leaf extract as anti-oxidant and anti-inflammatory material. Total polyphenol and total flavonoid content of Psidium guajava leaf extract was checked. Cream having inhibitory effect on inflammation through toxicity and NO production inhibition in RAW 264.7 cell was manufactured, and skin safety was evaluated. It was confirmen that total polyphenol and flavonoid content of Psidium guajava leaf extract was 126.4 mg/g and 223.17 mg/g respectively, which was high content. According to the results of checking toxicity through cell viability in RAW 264.7 cell, cytotoxicity was not shown. And NO production indicating inflammatory disease was inhibited concentration-dependently. According to the results of carrying out single patch test after manufacturing the cream containing the Psidium guajava leaf extract, skin irritation did not occur for 24 h to put patch on skin or for 24 h after removing the patch. Putting these results together, it was verified that there was possibility of application as raw materials for cosmetics, which would have anti-oxidant activity owing to the high polyphenol and flavonoid content of Psidium guajava leaf extract and anti-inflammatory material through NO production inhibition.
Keywords
cytotoxicity; flavonoids; nitric oxide; polyphenol; Psidium guajava leaf;
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1 Moreno MI, Isla MI, Sampietro AR, Vattuone MA, "Comparison of the free radical-scavenging activity of propolis from several regions of Argentina," J Ethnopharmacol., Vol. 71, pp. 109-114, (2000).   DOI
2 Agudelo Maria, Rodriguez Carlos A, Zuluaga Andres F, Vesga Omar, "Guidelines on in vivo tests and in vitro tests of cosmetic products," Korea. food. and drug. adminstration, (2011).
3 Chen HY, Yen GC, "Antioxidant activity and free radical-scavenging capacity of extracts from guava leaves," Food Chemistry., Vol. 101, pp. 686-694, (2007).   DOI
4 Choi SY, Lim SH, Kim JS, Ha TY, Kim SR, Kang KS, Hwang IK, "Evaluation of the estrogenic and antioxidant activity of some edible and medicinal plants," Korean J. Food Sci. Technol., Vol. 37, pp. 549-556, (2005).
5 Park BJ, Onjo M, “Anti-oxidant activities and tyrosinase inhibitory effects of guava (Psidium guajava L.) leaf,” Korean J. Plant Res., Vol. 21, No. 5, pp. 408-412, (2008).
6 Chen HY. Yen GC, "Antioxidant activity and free radical-scavenging capactiy of extracts from guava (Psidium guajava L.) leaves," Food Chem., Vol. 101, pp. 686-694, (2007).   DOI
7 Tachakittirungrod S, Okonogi S, "Chowwanapoonpahn S. Study on antioxidant activity of oertain plants in Thailand: Mechanism of antioxidant action of guava leaf extract," Food Chem. Vol. 103, No. 2, pp. 381-388, (2007).   DOI
8 Fransworth NR, Bunyapraphatsara N. "Thia Medicinal Plants Recommended for Primary Health Care in Thailand," Mahidol University. Bangkok, pp. 202-207, (1990).
9 Kim MH, "Anti-inflammatory effects of psidium guajava leaf extracts and its compounds in RAW 264.7 macrophages," Catholic University Msater's thesis, (2014).
10 Folin O, Denis W, "On phosphotungsticphosphomolybdic compounds as color reagents," J. Biolchem., Vol. 12, pp. 239-243, (1912).
11 Maines MD, "The oxygenase system: a regular of second messenger gases," Annu. Rev. Pharmacol Toxicol., Vol. 37, pp. 517-554, (1997).   DOI
12 Zedler S, Faist E, "The impact of endogenous triggers on trauma associated inflammation," Curr Opin Crit Care. Vol. 12., pp. 595-601, (2006).   DOI
13 Guha M, Mackman N, “The phosphatidylinositol 3-kinase-Akt pathway limits lipopolysaccharide activation of signaling pathways and expression of inflammatory mediators in human monocytic cells,” J. Biol chem., Vol. 277, No. 35, pp. 32124-32132, (2002).   DOI
14 Nathan PA, “Review of ergonomic studies of carpal tunnel syndrome,” AM. J. Ind. Med., Vol. 2, No. 6, pp. 895-897, (1992).
15 Shew RL, Papka RE, McNeill DL, Yee JA. "NADPH-diaphorase-positive nerves and the role of nitric oxide in CGRP relaxation of uterine contraction," Peptides. Vol. 14, pp. 637, (1993).   DOI
16 Kwqagkmata H, Ochiai H, Mantani N, Terasawa K, "Enhanced expression of inducible nitric oxide synthase by Juzen-taiho-to in LPS activated RAW 264.7 cells," a murinemacrophage cell line. Am. J. Chin. Med., Vol. 28, pp. 217, (2000).   DOI
17 Brown GC, "NO says yes to mitochondria," Cell Biol., Vol. 299, 838-839, (2003).
18 Iyengar R, Sutehr DJ, Marletta MA. "Macrophage synthesis of nitrite, nitrate and N-nitrosamines : Precursors and cytotoxic activities of pomegranate (Punica granatum) leaves," Food and Chem. Toxico., Vol. 55, pp. 470-475, (1987).
19 Snyder SH, Bredt DS, "Biological roles of nitric oxide," Sci. Am., Vol. 266, 68-77, (1992).
20 Weller R, "Nitric oxide-a newly discovered chemical transmitter in human skin," Br. J. Dermatol., Vol. 137., pp. 665, (1997).   DOI
21 Kim MJ, Lee SP, Choi JH, Kwon SH, Kim HD, Bang MH, Yang SA. "Characteristics of fermented dropwort extract and vinegar using fermented dropwort extract and its protective effects on oxidative damage in rat glioma C6 cells," Kor. J. Food Sci. Technol., Vol. 45, pp. 350-355, (2013).   DOI
22 You HJ, Hong SU, “A Case of Urticarial Drug Eruption Assumed to be Caused by non-steroidal anti-inflammatory drugs (NSAIDs),” J Korean Med. Ophthalmol Otolaryngol Dermatol., Vol. 20, No. 1, pp. 256-264, (2007).
23 You SH, Moon JS, “A Study on Anti-oxidative, Anti-inflammatory and Melanin Inhibitory Effects of Chrysanthemum Sibiricum Extract,” J. of Korean Oil Chemists's Soc., Vol. 33, No. 4, pp. 762-770, (2016).   DOI
24 You SH, Pyo YH, "Antioxidant and Anti-inflammatory Activities of Ethanol Extracts from Wheat Sprout," J Invest Cosmetol., Vol. 11, pp. 231-238, (2015).   DOI
25 Choi MH, "Whitening effects screening of Chenopodium album var. centrorubrum extracts as a functional cosmetics material," WonKwang University Doctorate thesis, (2014).
26 Cho YJ, "Study on the tyrosinase activity inhibition and free radical scavenging activity of natural materials," SookMyung University Msater's thesis, (2004).
27 Kim JH, "The Study on the Physiological Activities of Liriope platyphylla Flower Extract," Daegu Haany University Msater's thesis, (2013).
28 Han HS, Kim SY, Lim DJ, Hwang WK. Development of Whitening Cosmetic Ingredients from Cudrania tricuspidata Stem Extract," Asian J Beauty Cosmetol. Vol. 14, No. 3, pp. 317-328, (2016).   DOI
29 Kim EH, “A Study of Whitening Cosmetics from natural products,” Asian J Beauty Cosmetol., Vol. 4, No. 2, pp. 195-203, (2006).
30 Lee EJ. Hwang WK, "Research Paper : The Development of Anti-wrinkle Cosmeceutical Ingredients from Glehniae Radix cum Rhizoma," J. Kor. Soc. Cosm. Vol. 17, No. 1, pp. 127-133, (2011).
31 Miean KH, Mohamed S, "Flavonoid (myricetin, quercetin kaempferol, luteolin, and apigenin) content of edible tropical plants," J Agric Food Chem., Vol. 49, pp. 3106-3112, (2001).   DOI
32 Lee MY, Kim BA, Yang JC, “Effects of Extracts Derived from Red Ginseng Residue on Antioxidant Activity and Elastase Inhibition,” J. of Korean Oil Chemists's Soc., Vol. 33, No. 4, pp. 658-666, (2016).   DOI
33 Kim MJ, You MJ, “Inhibition of Melanogenesis and Anti-UV properties Reynoutria elliptica,” Asian J Beauty Cosmetol., Vol. 10, No. 4, pp. 961-968, (2012).
34 Kim CH, Kwon MC, Han JG, Na CS, Kwak HG, Choi KP, Park WY, Lee HY. "Skin-Whitening and UV-Protective Effects of Angelica gigas Nakai Extracts on Ultra High Pressure Extraction Process," KJMCS. Vol. 16, No. 4, pp. 255-260, (2008).
35 Begum S, Hassa SI, Siddiqui BS, Shaheen F, Ghayur MN, Gilani AH, "Triterpenoids from the leaves of Psidium guajava," Phytochemistry., Vol. 61, pp. 399-403, (2002).   DOI
36 Mercadante AZ, Steck A, Pfander H. "Carotenoids from guava (Psidium guajava L.) : isolation and structure elucidation," J Agric Food Chem., Vol. 47, pp. 145-151, (1999).   DOI
37 Meckes M, Calzada F, Tortoriello J, Gonzalez JL, Martinez M, "Terpenoids isolated from Psidium guajava hexane extract with depressant activity on central nervous system," Phytother Res., Vol. 10, pp. 600-603, (1996).   DOI
38 Lozoya X, Meckes M, Abou-Zaid M, Tortoriello J, Nozzolillo J, Arnason JT. "Quercetin glycosides in Psidium guajava L. leaves and determination of spasmolytic principle," Arch. Med. Res., Vol. 25, pp. 11-15, (1994).
39 Jaiarj P, Wongkrajang Y, Thongpraditchote S, Peungvicha P, Bunyapraphatsara N, Opartkiattikul N, "Guava leaf extract and topical haemostasis," Phytother Res. Vol. 14, pp. 388-391, (2000).   DOI
40 Kim MJ, Choi HY, Kim SI, "Quality Characteristics and Antioxidative Activities of Guavapyun Added Korean Guava Fruit Extract," Korean J. Food Cookery sci. Vol. 26, pp. 246-251, (2010).
41 Jeong SA, "Antioxidant and Antimicrobial Activities of Guajava Leaf (Psidium guajava) depending on Extract Solvent," International University, (2012).
42 You SH, "Antioxidant Activity and Whitening activity of Psidium guajava leaf extract. J. of Korean Oil Chemists's Soc. Vol. 34, No. 2, pp. 296-304, (2017).
43 Tanaka T, Ishida N, Ishimatsu M, Nonaka G, Nishioka I, "Tannins and related compounds. CXVI. Six new complex tannins, guajavins, psidinins and psiguavin from the bark of Psidium guajava L," Chem. Pharm. Bull., Vol. 40, pp. 2092-2098, (1992).   DOI